Zinc detoxification by a cyanobacterium from a metal contaminated bay in Brazil

Andrade, Luciana Segura de; Keim, Carolina N.; Farina, Marcos; Pfeiffer, Wolfgang C.

doi:10.1590/s1516-89132004000100020

Cited by 21 publications

(12 citation statements)

References 22 publications

(19 reference statements)

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“…Taxa that were identified as sensitive to multiple metals included most Crenarchaeota and Methanomicrobia (Archaea); and Cyanobacteria, Chloroflexi, Pseudoalteromonadaceae, and Verrucomicrobiales DEV007 (Bacteria). However, some of these sensitive taxa (particularly Cyanobacteria) have been previously well described as metal tolerant , and the metal susceptibility profiles described in the present study may have been influenced by confounding factors, as discussed below in the section Confounding factors . Because of the complexity of these sediments, and the potential for confounding factors that may bias analysis, culture and resistance testing of organisms identified in this screening process are required to confirm metal resistance profiles.…”

Section: Discussionmentioning

confidence: 78%

Molecular screening of microbial communities for candidate indicators of multiple metal impacts in marine sediments from northern Australia

Cornall

Rose

Streten

et al. 2016

Enviro Toxic and Chemistry

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Coastal sediments accumulate metals from anthropogenic sources and as a consequence industry is required to monitor sediment health. The total concentration of a metal does not necessarily reflect its potential toxicity or biological impact, so biological assessment tools are useful for monitoring. Rapid biological assessment tools sensitive enough to detect relatively small increases in metal concentrations would provide early warning of future ecosystem impact. The authors investigated in situ populations of Archaea and Bacteria as potential tools for rapid biological assessment in sediment at 4 northern Australian coastal locations over 2 yr, in both wet and dry seasons. The 1 M HCl-extractable concentrations of metals in sediment were measured, and Archaeal and Bacterial community profiles were obtained by next-generation sequencing of sediment deoxyribonucleic acid (DNA). Species response curves were used to identify several taxonomic groups with potential as biological indicators of metal impact. Spatial variation, sediment grain size, water depth, and dissolved oxygen also correlated with microbial population shifts. Seasonal variation was less important than geographic location. Metal-challenge culture trials supported the identification of metal-resistant and -sensitive taxa. In situ Archaea and Bacteria are potentially sensitive indicators for changes in bioavailable concentrations of metals; however, the complexity of the system suggests it is important to identify metal-specific functional genes that may be informed by these sequencing surveys, and thus provide a useful addition to identity-based assays.

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Section: Discussionmentioning

confidence: 78%

Molecular screening of microbial communities for candidate indicators of multiple metal impacts in marine sediments from northern Australia

Cornall

Rose

Streten

et al. 2016

Enviro Toxic and Chemistry

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“…Polyphosphate bodies are storage granules for phosphate, forming when polyphosphate deposited in the surrounding cytoplasm penetrates into their porous structure (Jensen, 1969). Polyphosphate bodies have also been reported to trap metals such as zinc (Andrade et al, 2004), putatively as a mechanism of detoxifying the metal. In vitro, microcystins form complexes with divalent metal cations including zinc (Humble et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Immunogold localisation of microcystins in cryosectioned cells of Microcystis

Young

Thomson

Metcalf

et al. 2005

Journal of Structural Biology

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“…Acidocalcisomal poly P stores are important for resistance to heavy metals in some eukaryotes and prokaryotes (Hashemi et al 1994;Alvarez & Jerez 2004;Andrade et al 2004;Remonsellez et al 2006;Nagasaka & Yoshimura 2008). Thus, phosphate and heavy metal storage may represent an ancestral characteristic that was important for their evolutionary development.…”

Section: Introductionmentioning

confidence: 99%

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes

Docampo

Ulrich

Moreno

2010

Phil. Trans. R. Soc. B

102

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Acidocalcisomes are acidic electron-dense organelles, rich in polyphosphate (poly P) complexed with calcium and other cations. While its matrix contains enzymes related to poly P metabolism, the membrane of the acidocalcisomes has a number of pumps (Ca 2+ -ATPase, V-H + -ATPase, H + -PPase), exchangers (Na + /H + , Ca 2+ /H + ), and at least one channel (aquaporin). Acidocalcisomes are present in both prokaryotes and eukaryotes and are an important storage of cations and phosphorus. They also play an important role in osmoregulation and interact with the contractile vacuole complex in a number of eukaryotic microbes. Acidocalcisomes resemble lysosome-related organelles (LRO) from mammalian cells in many of their properties. They share similar morphological characteristics, acidic properties, phosphorus contents and a system for targeting of their membrane proteins through adaptor complex-3 (AP-3). Storage of phosphate and cations may represent the ancestral physiological function of acidocalcisomes, with cation and pH homeostasis and osmoregulatory functions derived following the divergence of prokaryotes and eukaryotes.

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Zinc detoxification by a cyanobacterium from a metal contaminated bay in Brazil

Abstract: ABSTRACT

Cited by 21 publications

References 22 publications

Molecular screening of microbial communities for candidate indicators of multiple metal impacts in marine sediments from northern Australia

Molecular screening of microbial communities for candidate indicators of multiple metal impacts in marine sediments from northern Australia

Immunogold localisation of microcystins in cryosectioned cells of Microcystis

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes

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