Effects of plant lectins on in vitro fibroblast proliferation

Abstract

Lectins are carbohydrate-binding proteins that have been isolated from various sources and presented a wide spectrum of biological activities. The effects of four lectins, namely, Phaseolus vulgaris phytohemagglutinin, PHA, wheat germ agglutinin, WGA, Artocarpus integrifolia seed lectins, jacalin and artocarpin, on in vitro fibroblasts proliferation were investigated. The lectins did not influence the initial cell adhesion to the plate. PHA and WGA at 10-20 µg/mL concentrations significantly decreased fibroblasts proliferation. At these concentrations, they caused morphological alterations on cells and over 80 µg/mL, promoted cell death. Neither jacalin nor artocarpin significantly affected cell proliferation.<br>O objetivo deste trabalho foi avaliar a influência das lectinas PHA, WGA, jacalina e artocarpina sobre a proliferação de fibroblastos in vitro. Para tanto, fibroblastos gengivais de voluntários saudáveis foram cultivados, por cinco dias, em DMEM suplementado com soro bovino fetal (10% v/v) e na presença das lectinas nas concentrações finais de 0.1 a 300 µg/mL. A adesão, o crescimento e a morfologia celular foram acompanhados por microscopia de inversão e contraste de fase. O índice de proliferação foi avaliado pelo método calorimétrico usando MTT. As lectinas não alteraram a adesão inicial dos fibroblastos à placa de poliestireno. PHA e WGA, nas concentrações de 10 a 20 µg/mL, diminuíram significativamente a proliferação celular. Nestas concentrações a morfologia celular é alterada e acima de 80 µg/mL, houve100% de morte celular. As lectinas jacalina e artocarpina não influenciaram a proliferação celular

Topical Application Effect of the Isolectin Hydrogel (Cramoll 1,4) on Second-Degree Burns: Experimental Model

“…Several studies have confirmed the use of lectins in the immune system activation, enlisting neutrophils through indirect mechanisms [ 41 ], promoting proinflammatory effects in polymorphonuclear cells and inducing the cytokines release [ 42 ], as well as triggering fibroblasts proliferation [ 43 ]. Previous assays accomplished by our group have shown a potential proinflammatory and immunomodulatory activity induced by Cramoll 1,4 lectin.…”

Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles

“…The ultrastructural analysis confirmed the integrity of follicles cultured in medium supplement with 10 µg/mL PHA. Previous dose response studies have shown that high concentrations of PHA (>80 µg/mL) have toxic effects on cultured fibroblasts, while low concentrations (0.1-10 µg/mL) have no positive effects 30 . In agreement with these data, the present study has demonstrated that the optimal concentration of PHA to stimulate gene expression and antrum formation in cultured preantral follicles is 10 µg/mL.…”

A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model

“…According to Nagase et al (2006) this MMP-9 participates in the phenomenon of wound healing, being responsible together with MMP-2 for metabolizing elastin, collagen type IV and other molecules of the ECM. It is known that lectins are chemoattracted by the inflammatory cells and, consequently, stimulate synthesis and secretion of several cytokines involved in the wound healing process (Sell and Costa 2003).…”