Cloning of a peroxidase gene from cassava with potential as a molecular marker for resistance to bacterial blight

Pereira, Luiz Filipe Protásio; Erickson, L.

doi:10.1590/s1516-89132003000200002

Cited by 11 publications

(9 citation statements)

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“…This finding agrees with what is known about cowpea's defense response mechanism to Xav, represented by a brown-red discoloration without complete collapse of the tissue (Gitaitis 1983). Plants employ a variety of defense mechanisms in response to pathogens, including the use of mechanical barriers, defense proteins and defensive enzymes (Pereira et al 2003). A role for proteins in cell wall structure in bacterial blight disease resistance has been reported by different researchers in several plant species.…”

Section: Discussionsupporting

confidence: 89%

“…Flood et al (1995) also suggested that peroxidases might play an important role in resistance to bacterial blight of cassava. A cationic peroxidase gene, MEPX1, was isolated from cassava and the DNA sequence of MEPX1 showed high homology with other plant peroxidase genes and contained a large intron typical of peroxidase genes (Pereira et al 2003). The amino acid sequence had 75% homology with two Arabidopsis thaliana peroxidases.…”

Section: Discussionmentioning

confidence: 99%

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Identification of markers associated with bacterial blight resistance loci in cowpea [Vigna unguiculata (L.) Walp.]

et al. 2010

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Cowpea bacterial blight (CoBB), caused by Xanthomonas axonopodis pv. vignicola (Xav), is a worldwide major disease of cowpea [Vigna unguiculata (L.) Walp.]. Among different strategies to control the disease including cultural practices, intercropping, application of chemicals, and sowing pathogen-free seeds, planting of cowpea genotypes with resistance to the pathogen would be the most attractive option to the resource poor cowpea farmers in sub-Saharan Africa. Breeding resistance cultivars would be facilitated by marker-assisted selection (MAS). In order to identify loci with effects on resistance to this pathogen and map QTLs controlling resistance to CoBB, eleven cowpea genotypes were screened for resistance to bacterial blight using 2 virulent Xav18 and Xav19 strains isolated from Kano (Nigeria). Two cowpea genotypes Danila and Tvu7778 were identified to contrast in their responses to foliar disease expression following leaf infection with pathogen. A set of recombinant inbred lines (RILs) comprising 113 individuals derived from Danila (resistant parent) and Tvu7778 (susceptible parent) were infected with CoBB using leaf inoculation method. The experiments were conducted under greenhouse conditions (2007 and 2008) and disease severity was visually assessed using a scale where 0 = no disease and 4 = maximum susceptibility with leaf drop. A single nucleotide polymorphism (SNP) genetic map with 282 SNP markers constructed from the same RIL population was used to perform QTL analysis. Using Kruskall-Wallis and Multiple-QTL model of MapQTL 5, three QTLs, CoBB-1, CoBB-2 and CoBB-3 were identified on linkage group LG3, LG5 and LG9 respectively showing that potential resistance candidate genes cosegregated with CoBB resistance phenotypes. Two of the QTLs CoBB-1, CoBB-2 were consistently confirmed in the two experiments accounting for up to 22.1 and to 17.4% respectively for the first and second experiments. Whereas CoBB-3 was only discovered for the first experiment (2007) with less phenotypic variation explained of about 10%. Our results represent a resource for molecular marker

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Identification of markers associated with bacterial blight resistance loci in cowpea [Vigna unguiculata (L.) Walp.]

et al. 2010

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“…Some of these genes show similarity to genes involved in defense mechanisms, homologs to R proteins, chitinase and receptor like protein kinases. Data generated through our studies on transcript profiling and by others (Pereira et al, 2001(Pereira et al, , 2003 will permit unraveling the complex pathway signal in the cassava-Xam interaction.…”

Section: Gene Expression Analysis By Transcript Profiling and Ests Anmentioning

confidence: 84%

“…In particular, a-and b-esterases and a peroxidase have been shown to be useful markers of pathogenesis with Xam (Pereira et al, 2001(Pereira et al, , 2003. In our laboratory, high throughput methods of transcript profiling have been developed to study cassava-Xam interactions and to annotate and determine gene functions.…”

Section: Gene Expression Analysis By Transcript Profiling and Ests Anmentioning

confidence: 99%

Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis–cassava interaction

et al. 2004

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Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is a widespread disease that affects cassava (Manihot esculenta Crantz). Studies on the pathogen population structure, pathogen diagnosis, identification and expression of plant genes involved in resistance have been carried out. Different molecular techniques were developed to assess the genetic diversity among the Xampopulations. Characterization of Xam population dynamics over time had enable us to determine the different factors that are associated with resistance breakdown and those that influence the genetic structure or virulence phenotypes of the pathogen's population. Methods for detecting the pathogen in vegetative planting materials and true seeds were developed and contributed to reduce the impact of the disease. To better understand the genetics of resistance a quantitative trait loci (QTLs) approach was developed. Using a PCR-based strategy with degenerate primers we isolated two resistance gene candidates in cassava. We also characterized a region of a chromosome rich in R-gene like sequence. In this review we also report the main results obtained by transcript profiling methodologies, cDNA-AFLP and ESTs developed by the authors to characterize the genes involved in disease resistance. All together these techniques allowed the identification of molecular markers either associated to CBB resistance or that may represent putative genes involved in disease resistance. This article reviews current knowledge on the molecular cassava-Xam interactions.

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“…Although the cassava– Xam interaction has been described as pathotype–cultivar specific (Restrepo et al ., 2000a, 2004), cassava bacterial blight resistance mapped to several QTLs located on different linkage groups, highlighting a complex interaction between this plant and the pathogen (Fregene et al ., 1997; Jorge et al ., 2000). Recently, differential expression of esterases and peroxidase have been shown in cassava infected with Xam (Pereira et al ., 2001, 2003). Still, little is known about the molecular mechanisms that lead to resistance to cassava bacterial blight, and therefore we looked for a broad, unbiased tool that would allow the detection of multiple genes putatively associated with resistance.…”

Section: Discussionmentioning

confidence: 99%

Identification of genes in cassava that are differentially expressed during infection with Xanthomonas axonopodis pv. manihotis

Santaella

Suárez

López

et al. 2004

Molecular Plant Pathology

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SUMMARY The cDNA-amplified fragment length polymorphism approach was used to identify differentially expressed transcripts from cassava infected by Xanthomonas axonopodis pv. manihotis (Xam). Approximately 3600 transcript-derived fragments (TDFs) were screened of which 340 were isolated. The nucleotide sequences of 250 TDFs were analysed and assembled into contigs and singletons. The amino acid sequences of their predicted products were compared with entries in databases and 63 of these clones showed homology to known plant genes. Of these, 32 showed similarity to plant defence proteins. Fifty-one TDFs corresponded to proteins of unknown function and 106 did not match any sequence in the public databases. Quantitative reverse transcription PCR was carried out with a selected set of gene transcripts that demonstrated an increase of expression during the infection. These results point out candidate genes that are associated with cassava resistance to Xam and reinforce the idea of a complex process occurring during this plant-pathogen interaction.

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Cloning of a peroxidase gene from cassava with potential as a molecular marker for resistance to bacterial blight

Abstract: Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis, is considered one of the most important bacterial diseases of cassava (Manihot esculenta Crantz

Cited by 11 publications

References 14 publications

Identification of markers associated with bacterial blight resistance loci in cowpea [Vigna unguiculata (L.) Walp.]

Identification of markers associated with bacterial blight resistance loci in cowpea [Vigna unguiculata (L.) Walp.]

Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis–cassava interaction

Identification of genes in cassava that are differentially expressed during infection with Xanthomonas axonopodis pv. manihotis

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