Studies on probiotics properties of two lactobacillus strains

Brizuela, María A.; Serrano, Paulina; Pérez, Yovanka

doi:10.1590/s1516-89132001000100013

Cited by 24 publications

(19 citation statements)

References 6 publications

(6 reference statements)

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“…When these values were compared to control (MRS), no statistical difference was observed p˂0.05. These results are in accordance with some studies that reported a doubling time of about 1 h for lactobacilli (Brizuela et al, 2001;Ayeni et al, 2011;Rezvani et al, 2016).…”

Section: Growth Of the Lactobacillus Speciessupporting

confidence: 93%

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Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

Mesquita¹,

Costa²,

Frutuoso³

et al. 2017

Afr. J. Microbiol. Res.

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The emergence of nearly untreatable infections caused by multidrug-resistant bacteria has led to a new public health concern in which a need for development of alternative non-antibiotic strategies has become urgent. The activity of metabolites produced by new strains of Lactobacillus against multidrugresistant bacteria was investigated. The objective of this work was to isolate and identify lactobacilli from artisanal kefir by 16S rRNA gene sequencing as well as to evaluate the effect of the growth of Lactobacillus species in the Man Rogosa and Sharpe broth, supplemented with Tween 80 at concentrations of 0.6, 0.4, and 0.2 %. Cell Free Supernatants (CFSs) were obtained from these lactobacilli cultures and with them, organic acids (lactic acid and acetic acid) and ethanol were quantified by HPLC followed by the determination of their antimicrobial activities against eight strains of Staphylococcus aureus-MRSA and Klebsiella pneumonia KPC strains. The GenBank BLAST analysis revealed that, the isolated lactobacilli belonged to Lactobacillus paracasei (n=4) and Lactobacillus plantarum (n=5) strains. Increasing concentrations of Tween 80 did not affect the growth of Lactobacillus species significantly when compared to their controls (MRS broth). HPLC analysis of CFSs showed concentrations greater than 18.0, 4.0 and 1.0 g/L for lactic acid, acetic acid and ethanol, respectively. All CFSs were able to inhibit all pathogenic microorganisms evaluated. The percentage of inhibition was on average greater than 88% for MRSA and KPC strains. The antimicrobial activity was dependent on the CFSs tested. Based on these experimental conditions, organic acids and ethanol are likely to be responsible for this antimicrobial activity.

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Section: Growth Of the Lactobacillus Speciessupporting

confidence: 93%

“…The MRS broth was used as a control. Parameters related to growth as doubling time (g) and specific growth rates (μ) were calculated (Brizuela et al, 2001;Georgieva et al, 2014).…”

Section: Preparation Of Modified Man Rogosa and Sharpe (Mrs) Media Smentioning

confidence: 99%

Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

Mesquita¹,

Costa²,

Frutuoso³

et al. 2017

Afr. J. Microbiol. Res.

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“…Usually, an incubation time of 12 h is applied. However, in this work we have used lower incubation time (1 h) to avoid exceeding the generation time of LAB and yeasts, which according to the literature, in optimal conditions, is approximately 1.1 h for many species of LAB, and 2 h for the growth-faster yeast species (Brizuela et al 2001;Nagpal and Kaur 2011;Willey et al 2011). In this way, duplication of the microorganisms that are released is prevented and time is reduced.…”

Section: Enzymatic Methodsmentioning

confidence: 99%

Evaluation and identification of poly-microbial biofilms on natural green Gordal table olives

Benítez-Cabello

Romero‐Gil

Rodríguez‐Gómez

et al. 2015

Antonie van Leeuwenhoek

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This work examines the formation of poly-microbial communities adhered to the epidermis of natural green Gordal olives and the application of different methodologies for recovery and counting of the microorganisms embedded in olive biofilms. The fermentation process was physicochemical and microbiologically monitored for 90 days, at which, formation of true biofilms on the skin of fermented fruits was confirmed by scanning electron microscopy. Then, samples of olives were taken and treated with sonication, enzymes, mechanical homogenization with stomacher and ultrasonic bath for biofilm disaggregation. The use of the stomacher for 1 min was the most effective treatment to release the lactic acid bacteria (6.6 log10 cfu g(-1)), whereas sonication for 5 min was the most efficient method for quantification of yeasts (up to 3.5 log10 cfu g(-1)). Molecular identification of isolates obtained from natural Gordal olive biofilms revealed that Lactobacillus pentosus was the only species found among lactic acid bacteria, while Pichia membranifaciens was the dominant yeast species, with higher counts obtained for the bacteria.

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“…Contrarily, a very poor prebiotic effect was reported for inulin by Shin et al (2000), likely due to the use of a particular Bifidobacterium strain. Also in this respect, reference to lactobacilli is very poor, and only a few attempts were made in pure cultures of L. acidophilus (Brizuela, Serrano, & Pé rez, 2001;Chou & Weimer, 1999;Du Plessis, Dicks, Vescovo, Torriani, & Dellaglio, 1996;Ordonez, Fung, & Jeon, 2000), Lactobacillus rhamnosus (Goldin, 1998;Jyoti, Suresh, & Venkatesh, 2004), Lactobacillus bulgaricus (Kimmel & Roberts, 1998;RadkeMitchell & Sandine, 1986), Lactobacillus plantarum and L. casei (Gonzá lez, Domínguez-Espinosa, & Alcocero, 2008).…”

Section: Introductionmentioning

confidence: 99%

Growth and acidification performance of probiotics in pure culture and co-culture with Streptococcus thermophilus: The effect of inulin

Oliveira

Perego

Converti

et al. 2009

LWT - Food Science and Technology

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Studies on probiotics properties of two lactobacillus strains

Abstract: ABSTRACT

Cited by 24 publications

References 6 publications

Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

Evaluation and identification of poly-microbial biofilms on natural green Gordal table olives

Growth and acidification performance of probiotics in pure culture and co-culture with Streptococcus thermophilus: The effect of inulin

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