Biochemical taxonomy of Crenicichla (Pisces: Perciformes: Cichlidae) of the Iguaçu River, Brazil

Renesto, Erasmo; Zawadzki, Cláudio Henrique; Revaldaves, Eloísa

doi:10.1590/s1516-89132001000100003

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…The A. altiparanae phenotypes we obtained for the 13 enzymatic systems studied were similar to those previously demonstrated for other Characiformes from the PR basin (Revaldaves et al, 1997;Renesto et al, 1997Renesto et al, , 2001Chiari and Sodré, 1999;Peres et al, 2002) and for Siluriformes (Almeida and Sodré, 1998;Zawadzki et al, 1999Zawadzki et al, , 2000aZawadzki et al, , 2000bRenesto et al, 2000). Our data revealed that A. altiparanae carries high genetic variability, 52.38% of polymorphic loci in PR and 38.10% in the RF.…”

Section: Discussionsupporting

confidence: 89%

“…Low estimated expected heterozygosity has also been reported for two other Iguaçu River fish, 0. 063 for Crenicichla iguassuensis (Renesto et al, 2001) and 0.024 for Pimelodus ortmanni (Renesto et al, 2000). These low expected heterozygosity values for Iguaçu River species may be due to regulation of invariable structural genes which guarantee phenotypic plasticity (Chippari-Gomes et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil

Peres

Vasconcelos²,

Renesto

2005

Genet. Mol. Biol.

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Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase -NADP + , 1.1.1.42 (IDH), L-Lactate dehydrogenase, 1.1.1.27 (LDH), Malate dehydrogenase, 1.1.1.37 (MDH), Malate dehydrogenase -NADP + , 1.1.1.40 (MDHP), Phosphoglucomutase, 5.4.2.2 (PGM), and Superoxide dismutase, 1.15.1.1 (SOD). The proportion of polymorphic loci were estimated as 52.38% in the PR population and 38.10% in the RF population. Expected estimated heterozygosities were 0.1518 ± 0.0493 for the PR population and 0.0905 ± 0.0464 for the RF population. The A. altiparanae heterozygosity data were similar to previous estimates for other PR basin characid species. Allele frequencies were significantly different between the PR and RF populations in respect to some loci (Acp-1, G3pdh-1, Gpi-A, Iddh-1, Mdhp-1 and Mdhp-2). Wright's statistics for all loci were estimated as F is = 0.3919, F it = 0.4804 and F st = 0.1455. Our results show that the A. altiparanae populations studied are genetically different and have a high degree of genetic variability.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil

Peres

Vasconcelos²,

Renesto

2005

Genet. Mol. Biol.

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“…It has evolved independently in different environments and species complexes of various New World (for example [ 50 ]) and Old World cichlids ([ 51 ] and references therein; [ 17 , 52 , 53 ]). It is considered to be a diagnostic trait: among the scores of described cichlid species (1611 Cichlidae in FishBase.org, 5 November 2009), we know of none for which the character of hypertrophied lips is a described intraspecific polymorphism (though there is debate about Crenicichla iguassuensis , see [ 54 , 55 ]).…”

Section: Discussionmentioning

confidence: 99%

Rapid sympatric ecological differentiation of crater lake cichlid fishes within historic times

et al. 2010

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BackgroundAfter a volcano erupts, a lake may form in the cooled crater and become an isolated aquatic ecosystem. This makes fishes in crater lakes informative for understanding sympatric evolution and ecological diversification in barren environments. From a geological and limnological perspective, such research offers insight about the process of crater lake ecosystem establishment and speciation. In the present study we use genetic and coalescence approaches to infer the colonization history of Midas cichlid fishes (Amphilophus cf. citrinellus) that inhabit a very young crater lake in Nicaragua-the ca. 1800 year-old Lake Apoyeque. This lake holds two sympatric, endemic morphs of Midas cichlid: one with large, hypertrophied lips (~20% of the total population) and another with thin lips. Here we test the associated ecological, morphological and genetic diversification of these two morphs and their potential to represent incipient speciation.ResultsGene coalescence analyses [11 microsatellite loci and mitochondrial DNA (mtDNA) sequences] suggest that crater lake Apoyeque was colonized in a single event from the large neighbouring great lake Managua only about 100 years ago. This founding in historic times is also reflected in the extremely low nuclear and mitochondrial genetic diversity in Apoyeque. We found that sympatric adult thin- and thick-lipped fishes occupy distinct ecological trophic niches. Diet, body shape, head width, pharyngeal jaw size and shape and stable isotope values all differ significantly between the two lip-morphs. The eco-morphological features pharyngeal jaw shape, body shape, stomach contents and stable isotopes (δ15N) all show a bimodal distribution of traits, which is compatible with the expectations of an initial stage of ecological speciation under disruptive selection. Genetic differentiation between the thin- and thick-lipped population is weak at mtDNA sequence (FST = 0.018) and absent at nuclear microsatellite loci (FST < 0.001).ConclusionsThis study provides empirical evidence of eco-morphological differentiation occurring very quickly after the colonization of a new and vacant habitat. Exceptionally low levels of neutral genetic diversity and inference from coalescence indicates that the Midas cichlid population in Apoyeque is much younger (ca. 100 years or generations old) than the crater itself (ca. 1 800 years old). This suggests either that the crater remained empty for many hundreds of years after its formation or that remnant volcanic activity prevented the establishment of a stable fish population during the early life of the crater lake. Based on our findings of eco-morphological variation in the Apoyeque Midas cichlids, and known patterns of adaptation in Midas cichlids in general, we suggest that this population may be in a very early stage of speciation (incipient species), promoted by disruptive selection and ecological diversification.

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“…These are different molecular forms of an enzyme catalyzing the same reaction in the cell ( Alfenas, 2006 ). Isozyme electrophoresis has been used with success to settle doubts regarding the taxonomic status of undescribed species of the Brazilian ichthyofauna ( Renesto et al , 2000 , 2001 , 2007 ; Zawadzki et al , 2000 , 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Biochemical comparison of two Hypostomus populations (Siluriformes, Loricariidae) from the Atlântico Stream of the upper Paraná River basin, Brazil

2009

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Two syntopic morphotypes of the genus Hypostomus - H. nigromaculatus and H. cf. nigromaculatus (Atlântico Stream, Paraná State) - were compared through the allozyme electrophoresis technique. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, attributing the score of 20 loci, with a total of 30 alleles. Six loci were diagnostic (Aat-2, Gcdh-1, Gpi-A, Idh-1, Ldh-A and Mdh-A), indicating the presence of interjacent reproductive isolation. The occurrence of few polymorphic loci acknowledge two morphotypes, with heterozygosity values He = 0.0291 for H. nigromaculatus and He = 0.0346 for H. cf. nigromaculatus. FIS statistics demonstrated fixation of the alleles in the two morphotypes. Genetic identity (I) and distance (D) of Nei (1978) values were I = 0.6515 and D = 0.4285. The data indicate that these two morphotypes from the Atlântico Stream belong to different species.

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Biochemical taxonomy of Crenicichla (Pisces: Perciformes: Cichlidae) of the Iguaçu River, Brazil

Cited by 8 publications

References 17 publications

Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil

Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil

Rapid sympatric ecological differentiation of crater lake cichlid fishes within historic times

Biochemical comparison of two Hypostomus populations (Siluriformes, Loricariidae) from the Atlântico Stream of the upper Paraná River basin, Brazil

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