Effects of fatty acids on carbohydrates and lipids of canola seeds during germination

Ferrarese, M. L. L.; Baleroni, C. R. S.; Ferrarese-Filho, Osvaldo

doi:10.1590/s1516-89131998000300007

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…An increase in the FA pool of EA and cotyledon was seen in 28 dai in this study. Similar findings were observed in canola, alfalfa, and soybean seeds, where total FA content increased once the second germination phase concluded ( Beevers, 1961 ; Ferrarese et al, 1998 ; Zhou et al, 2019 ).…”

Section: Discussionsupporting

confidence: 83%

The Enigmatic Aliphatic Acetogenins and Their Correlations With Lipids During Seed Germination and Leaf Development of Avocado (Persea americana Mill.)

et al. 2022

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Lipids in avocados have been widely studied due to their nutritional value and several reported bioactivities. Aliphatic acetogenins are a relevant component of the avocado lipidome and have been tested for several potential food and pharma industries applications. This work followed the evolution of avocado fatty acids (FAs) and aliphatic acetogenins during seed germination and leaf growth. Oil extracts of embryonic axes, cotyledons, and leaves from seedlings and trees were divided to analyze free acetylated acetogenins (AcO-acetogenins), and free FAs. Embryonic axes from germinating seeds contained the highest amount of AcO-acetogenins and FAs; this tissue also accumulated the most diverse FA profile with up to 22 detected moieties. Leaves presented the highest variations in AcO-acetogenin profiles during development, although leaves from seedlings accumulated the simplest FA profile with only 10 different FAs. Remarkably, AcO-acetogenins represented half of the carbons allocated to lipids in grown leaves, while embryonic axes and cotyledons always contained more carbons within FAs during germination. Thus, we hypothesized the use of the AcO-acetogenin acyl chain for energy production toward β-oxidation. Also, α-linolenic and docosahexaenoic acids (DHAs) were proposed as close AcO-acetogenin intermediaries based on a correlation network generated using all these data. Another part of the oil extract was fractionated into different lipid classes before transesterification to profile FAs and acetogenins bound to lipids. Acetogenin backbones were identified for the first time in triglycerides from cotyledons and mainly in polar lipids (which include phospholipids) in all developing avocado tissues analyzed. Seed tissues accumulated preferentially polar lipids during germination, while triglycerides were consumed in cotyledons. Seedling leaves contained minute amounts of triglycerides, and polar lipids increased as they developed. Results from this work suggest acetogenins might be part of the energy and signaling metabolisms, and possibly of membrane structures, underlining the yet to establish role(s) of these unusual lipids in the avocado plant physiology.

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Section: Discussionsupporting

confidence: 83%

The Enigmatic Aliphatic Acetogenins and Their Correlations With Lipids During Seed Germination and Leaf Development of Avocado (Persea americana Mill.)

et al. 2022

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“…Toxic effects of fatty acids on plant germination have been documented in various studies [55,56]. The effects of medium-chain fatty acids on seed germination have been linked to changes in the physical properties of membranes, which alters the kinetics of their associated enzymes, affecting water potential and ionic exchange of the embryonic axes [57,58]. We suggest that prior to the use of any fatty acid as a plant protection agent, phytotoxicity tests must be carried out on the plant species in question.…”

Section: Discussionmentioning

confidence: 99%

Medium-Chain Fatty Acids from Eugenia winzerlingii Leaves Causing Insect Settling Deterrent, Nematicidal, and Phytotoxic Effects

et al. 2019

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Eugenia winzerlingii (Myrtaceae) is an endemic plant from the Yucatan peninsula. Its organic extracts and fractions from leaves have been tested on two phloem-feeding insects, Bemisia tabaci and Myzus persicae, on two plant parasitic nematodes, Meloidogyne incognita and Meloidogyne javanica, and phytotoxicity on Lolium perenne and Solanum lycopersicum. Results showed that both the hexane extract and the ethyl acetate extract, as well as the fractions, have strong antifeedant and nematicidal effects. Gas chromatography-mass spectrometry analyses of methylated active fractions revealed the presence of a mixture of fatty acids. Authentic standards of detected fatty acids and methyl and ethyl derivatives were tested on target organisms. The most active compounds were decanoic, undecanoic, and dodecanoic acids. Methyl and ethyl ester derivatives had lower effects in comparison with free fatty acids. Dose-response experiments showed that undecanoic acid was the most potent compound with EC50 values of 21 and 6 nmol/cm2 for M. persicae and B. tabaci, respectively, and 192 and 64 nmol for M. incognita and M. javanica, respectively. In a phytotoxicity assay, medium-chain fatty acids caused a decrease of 38–52% in root length and 50–60% in leaf length of L. perenne, but no effects were observed on S. lycopersicum. This study highlights the importance of the genus Eugenia as a source of bioactive metabolites for plant pest management.

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“…So, there might be difficult for the water to reach germplasm of the float seeds. Thereby, the germination of float seeds could not be accomplished [44]. As a result of germination, it was understood that mature sink seeds break down their stored lipids for complete development.…”

Section: Discussionmentioning

confidence: 99%

“…The lipids are highly non-polar hydrocarbons which are insoluble in water as compared to polar substances like glucose, which solubilise with a saturation point of 1.1 mg/g of water. Due to the presence of higher lipid, it makes them float; further, it blocks the entry of water into the germplasm through the testa [44]. A significantly higher percentage of germination can be related to the presence of a higher total protein content in the sink seeds ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Germinating potential of Phyllanthus amarus seeds: evaluation of biochemical parameters

et al. 2019

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Collection, authentication, optimisation of storage conditions and selection of suitable propagules for propagation and conservation are crucial in the case of wild-growing medicinal plants which have adaptive variations under different climate regimes. The present study aims to understand the factors (here, seed types and storage conditions) that influence the germination potential of wild-collected Phyllanthus amarus L. (Euphorbiaceae) using biochemical characteristics of seeds, besides taxonomical authentication of the species. The amplicons of 1150 bp in SCAR marker analysis and 6-7 ribs on seed surface shown by scanning electron micrograph confirmed that the wild-collected specimen is P. amarus. Interestingly, the mature green seeds harvested and shade-dried within 0-3 days exhibited maximum sink percentage (55.8%) and showed a significantly (p < 0.001) higher germination percentage with carbohydrate and proteins than the float seeds. However, the float seeds contained 33% less fat content than the sink seeds. Upon storage, the germinability of P. amarus seeds was found to be 12 months at 4 °C without moisture. This shows that viable seeds can be harvested and stored appropriately to ensure longevity. This study makes a case for the preservation of seasonal, short lifespan seeds such as P. amarus.

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Effects of fatty acids on carbohydrates and lipids of canola seeds during germination

Cited by 8 publications

References 16 publications

The Enigmatic Aliphatic Acetogenins and Their Correlations With Lipids During Seed Germination and Leaf Development of Avocado (Persea americana Mill.)

The Enigmatic Aliphatic Acetogenins and Their Correlations With Lipids During Seed Germination and Leaf Development of Avocado (Persea americana Mill.)

Medium-Chain Fatty Acids from Eugenia winzerlingii Leaves Causing Insect Settling Deterrent, Nematicidal, and Phytotoxic Effects

Germinating potential of Phyllanthus amarus seeds: evaluation of biochemical parameters

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