Comparação da eficiência de métodos de inoculação na avaliação da patogenicidade de isolados de colletotrichum gloeosporioides em frutos de maracujá (Passiflora Edulis)

Abstract

Eight inoculation methods were studied to evaluate the pathogenicity among six isolates of Colletotrichum gloeosporioides, agent of anthracnose on passion fruits (Passiflora edulis). The isolates were selected by micelial growth and sporulation. The inoculations were made through suspension (1x10 6 conidia/ml) and micelial-agar discs (4mm in diameter). After 6 days of inoculation, the diameter of necrotic area was measure on the fruit epiderm. The most efficient inoculation method was the micelialagar disc on the fruit wounded epiderm. All isolates caused necrosis by this method, but it showed low efficiency using intact epiderm, where only one isolate was active. One isolate from stem showed greater pathogenicity than the others, and the necrosis in the area was ten times larger than the less pathogenic isolates. The results showed that more than two inoculation methods must be used for the isolate evaluation.