Analisador automático hematológico e a importância de validar novos equipamentos em laboratórios clínicos

Bacall, Nydia S.

doi:10.1590/s1516-84842009000400006

Cited by 6 publications

(5 citation statements)

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“…The software was programmed to recognize leukocytes but can also be programmed to recognize any object. The utility of this design includes the capability to recognize various characteristics of the cells (i.e., blasts, atypical lymphocytes, and immature granulocytes), which certain devices do not detect (BACALL, 2009). The software would also be able to identify diseases such as acute leukemia, which displays pathognomonic changes in morphology of the cells (CRAIG and FOON, 2008).…”

Section: Discussionmentioning

confidence: 99%

A reproducible differential blood cell count without a cytometer or specific markers

et al. 2015

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Introduction: A differential blood cell count represents a substantial component of the daily clinical routine. The aim of this study was to conduct automated high-throughput analyses of light microscopy images of leukocytes with unspecific staining (hematoxylin-eosin, HE) and to classify the resulting subgroups (lymphocytes, monocytes and neutrophils). Materials and Method: The software package CellProfiler was used for the image analysis and cell classification. The results were compared to those from hematological laboratories that analyzed the same samples. Results: There was a high similarity between the results obtained by image analysis and those from the hematological laboratories, with an r2=0.95 for the differential count and an r2=0.89 for the total count. Conclusion: Differential blood cell count using light microscopy, nonspecific dyes and advanced image analysis is accurate, reproducible, and less expensive compared to other techniques. The results also showed a strong correlation when compared to traditional methods.

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Section: Discussionmentioning

confidence: 99%

A reproducible differential blood cell count without a cytometer or specific markers

et al. 2015

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“…Os equipamentos utilizados para a análise foi um contador de células automatizado, da marca HUMAN, modelo HumaCont 30 TS. O equipamento é um contador de células hematológicas de bancada totalmente automatizado, que implementa o chamado método Coulter primeiramente descrito em 1956 por Wallace Coulter para contagem de células que passam por uma pequena abertura e mede o conteúdo de hemoglobina dos glóbulos vermelhos (BACALL, 2009). O analisador possui um módulo de display LCD gráfico colorido com tela de toque, e tem um botão INICIAR separado conforme a figura 1.…”

Section: Methodsunclassified

Engenharia Clinica: A importância da manutenção preventiva em equipamentos laboratoriais

Jesus¹,

Pinheiro²,

Alves³

et al. 2021

Engenharia Elétrica: As Contribuições Da Vida Acadêmica No Cotidiano Do Profissional

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“…The determination of the red blood cell distribution width (RDW-CV) and its fractions as well as erythrocyte, haematocrit (Ht), haemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total leukocyte and blood platelet count values were obtained using the haematological analyser model POCH-100iV DIFF (Sysmex from Brazil Industry and Commerce Ltd., Curitiba/PR-Brazil) (BACALL, 2009;RIOND et al, 2011). To obtain the total and differential count of leukocytes, a blood smear stained with fast staining (Diff-Quick) was performed according to Jain (1993).…”

Section: Laboratorial Exams Haemogrammentioning

confidence: 99%

Cardiorespiratory and haematologicalparameters of healthy french bulldogs

et al. 2021

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Brachycephalic dogs have a normal lower jaw and a receded upper jaw, and these dogs are predisposed to nostril stenosis, elongated palate, tracheal hypoplasia and hyperthermia due to heat stress. Giventhese characteristics, these dogs are more predisposed to respiratory and cardiovascular disorders. The popularity of brachycephalic dogsthathave a greatertendency for cardiorespiratory disorders, such as French bulldogs,has increased recently; however, studies on the cardiorespiratoryand haematological parameters in this breedare lacking. Thus, the present study sought to evaluatethe cardiorespiratory and haematological parameters of healthy French bulldogs to standardize normal values forthisbreed. Thirty-six French bulldogs wereused without predilection for age or sex, and the following parameters were evaluated: temperature (T), heart rate (HR), respiratory rate (RR), erythrogram, leukogram, total plasma protein, plasmatic fibrinogen, blood platelet count, urea, creatinine, alkaline phosphatase (AP) and alanine aminotransferase (ALT). The mean and standard deviation wereas follows: T°C (38.3±0.4), HR (111.1±27.0) beats per minute, RR (54.8±32.5) breaths per minute, erythrocyte count (7.3±1.2x106), haemoglobin (16.9±2.8 g/dL), haematocrit (48.9±7.7%), MCV (67.6±2.4fL), MCH (23.3±0.7 pg), MCHC (34.6±0.8%), RDW-CV (9.3±0.9; 9.2-12.0%), total leukocytes (11009.7±2907.3/mm3), band neutrophils (250.0±260.4/mm3), segmented neutrophils (7094.4±2307.3/mm3), eosinophils (589.7±561.3/mm3), lymphocytes (2020.8±1457.5/mm3), monocytes (987.6±528.4/mm3), total plasma protein (6.9±0.5 g/dL), plasmatic fibrinogen (274.1±96.5 mg/dL), blood platelet count (366.4±88.5/mm3), urea (35.2±12.6 mg/dL), creatinine (0.8±0.2/mg/dL), AP (49.1±25.8U/L),ALT (36.2±12.3U/L). The haematological parameters and heart rate were within the normal values for the species, but the respiratory rate values (54.8±32.5 breaths per minute)were greater than the normal averageof10 to 30 breaths per minute. We conclude thatthehaematological parameters and heart rateof healthy French bulldogs arewithin the normal limit for dogs; however, the respiratory rate is above referencevalues, reinforcing the notion that dogs of this breed, although healthy,exhibitan increasedrisk of respiratory disorders

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Analisador automático hematológico e a importância de validar novos equipamentos em laboratórios clínicos

Cited by 6 publications

References 0 publications

A reproducible differential blood cell count without a cytometer or specific markers

A reproducible differential blood cell count without a cytometer or specific markers

Engenharia Clinica: A importância da manutenção preventiva em equipamentos laboratoriais

Cardiorespiratory and haematologicalparameters of healthy french bulldogs

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