Detecção de Aspergillus sp pela técnica de PCR-nested em pacientes submetidos a transplante de medula óssea

Abstract

Invasive aspergillosis is an important cause of mortality in longterm neutropenic patients, particularly after bone marrow (B.M.T.) and solid organ transplantation. More than 90% of invasive fungal infections in immunocompromised patients can be attributed to Candida and Aspergillus. To date, there is no fast, reliable and feasible test for Aspergillus infection detection in routine procedures. In the present report, a nested PCR technique, developed to detect Aspergillus infection, is described. In our study, the sensitivity, specificity, positive and negative values using this approach were

Diagnosis of invasive aspergillosis: Application of Polymerase Chain Reaction Techniques (PCR) and Enzyme-Linked Immunosorbent Assay for Detection of Galactomannan (EIA-GM®)

“…Despite the relatively small sample, this study presented relevant and consistent results with other published studies, showing that this technique may contribute to the decision-making process of clinical staff to the current situation of AI. 30,32 Novaretti et al 31 also showed that the PCR-Nested test has considerable clinical potential for the early diagnosis of AI. This research aimed to standardize the Nested PCR for the detection of Aspergillus spp in 23 leukemia patients.…”

“…Furthermore, different studies demonstrate that false-positive results can also occur in patients affected by other systemic mycoses because the epitope detected by the monoclonal antibody used in the Platelia GM® EIA-test is also present in other similar antigens and some fungal species, being them: ■ genera: Paecilomyces, Alternaria, Trychophyton, Botrytis, Wallemia, Cladosporium, Geotrichum, Trichothecium, Myceliophthora, Blastomyces, and Fusariume infections caused by Neosartoria; 4,16,17,23,[29][30][31] ■ species: Paracoccidioides brasiliensis, Histoplasmacapsulatum, Cryptococcusneoformans e Cryptococcusgattii, Geotrichumcapitatum, Penicilliummarneffei. 15,29 The high mortality rate of the disease in high-risk populations reflects, in part, the difficulty in establishing a reliable early diagnosis and the rapid and accurate identification necessary for the successful clinical management of infection.…”