Salmonella spp. in meat-type quails (Coturnix coturnix coturnix) in the State of São Paulo, Brazil

Neto, Oliveiro Caetano de Freitas; Angela, HL da; Soares, NM; Guastalli, Elisabete Aparecida Lopes; Almeida, AM; Berchieri, Ângelo

doi:10.1590/s1516-635x2013000300016

Cited by 3 publications

(1 citation statement)

References 31 publications

(29 reference statements)

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“…The observed Salmonella prevalence among turkeys (4.17%) was lower than that previously reported in German fattening flocks (10.3%) (Käsbohrer et al, 2013). In contrast to the reported prevalence of Salmonella among quails (3.85%), a very high rate (75%) has been declared in Brazil (de Freitas Neto et al, 2013). These variations may be attributed to the geographical areas, climatic conditions, poultry species or breed, sample types, and disparity in sampling procedure and Salmonella isolation protocol.…”

Section: Discussioncontrasting

confidence: 66%

Extensive Drug-Resistant Salmonella enterica Isolated From Poultry and Humans: Prevalence and Molecular Determinants Behind the Co-resistance to Ciprofloxacin and Tigecycline

et al. 2021

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The emergence of extensive drug-resistant (XDR) Salmonella in livestock animals especially in poultry represents a serious public health and therapeutic challenge. Despite the wealth of information available on Salmonella resistance to various antimicrobials, there have been limited data on the genetic determinants of XDR Salmonella exhibiting co-resistance to ciprofloxacin (CIP) and tigecycline (TIG). This study aimed to determine the prevalence and serotype diversity of XDR Salmonella in poultry flocks and contact workers and to elucidate the genetic determinants involved in the co-resistance to CIP and TIG. Herein, 115 Salmonella enterica isolates of 35 serotypes were identified from sampled poultry (100/1210, 8.26%) and humans (15/375, 4.00%), with the most frequent serotype being Salmonella Typhimurium (26.96%). Twenty-nine (25.22%) Salmonella enterica isolates exhibited XDR patterns; 25 out of them (86.21%) showed CIP/TIG co-resistance. Exposure of CIP- and TIG-resistant isolates to the carbonyl cyanide 3-chlorophenylhydrazone (CCCP) efflux pump inhibitor resulted in an obvious reduction in their minimum inhibitory concentrations (MICs) values and restored the susceptibility to CIP and TIG in 17.24% (5/29) and 92% (23/25) of the isolates, respectively. Molecular analysis revealed that 89.66% of the isolates contained two to six plasmid-mediated quinolone resistance genes with the predominance of qepA gene (89.66%). Mutations in the gyrA gene were detected at codon S83 (34.62%) or D87 (30.77%) or both (34.62%) in 89.66% of XDR Salmonella. The tet(A) and tet(X4) genes were detected in 100% and 3.45% of the XDR isolates, respectively. Twelve TIG-resistant XDR Salmonella had point mutations at codons 120, 121, and 181 in the tet(A) interdomain loop region. All CIP and TIG co-resistant XDR Salmonella overexpressed ramA gene; 17 (68%) out of them harbored 4-bp deletion in the ramR binding region (T-288/A-285). However, four CIP/TIG co-resistant isolates overexpressed the oqxB gene. In conclusion, the emergence of XDR S. enterica exhibiting CIP/TIG co-resistance in poultry and humans with no previous exposure to TIG warrants an urgent need to reduce the unnecessary antimicrobial use in poultry farms in Egypt.

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Section: Discussioncontrasting

confidence: 66%

Extensive Drug-Resistant Salmonella enterica Isolated From Poultry and Humans: Prevalence and Molecular Determinants Behind the Co-resistance to Ciprofloxacin and Tigecycline

et al. 2021

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Incidence, species and antimicrobial resistance of naturally occurring Campylobacter isolates from quail carcasses sampled in a commercial processing facility

Cox

Cosby

Thippareddi

et al. 2018

Journal of Food Safety

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There is limited information about Campylobacter in commercially processed quail (Coturnix coturnix). The objective of this study was to determine the occurrence of Campylobacter on carcasses in a commercial quail processing plant. Carcasses were collected prior to chilling and/or the application of any antimicrobials, transported to the laboratory, and individually bagged. Following the standard protocol for recovering Campylobacter, three typical colonies were selected, confirmed as Campylobacter and tested for antimicrobial susceptibility. Nine replicates were conducted. Of the 85 carcasses obtained (n = 5 for first visit, n = 10 for 8 subsequent visits), 28 (32.9%) carcasses were found to be positive for Campylobacter. On four visits, no Campylobacter was found; on two visits, all samples were positive; and 1/5, 1/10, and 6/10 samples were positive for the remaining visits. Of the 28 isolates recovered, 18 (64%) were C. jejuni and 10 (36%) were C. coli. Tetracycline resistance was detected in all 28 isolates. Future work in the breeder flocks, hatchery, and grow‐out houses is planned to further understand Campylobacter ecology in quail production and processing which could also be useful to the broiler industry.Practical applicationsVery little published information exists for the presence of Campylobacter spp. on processed quail. Most of the studies involve cloacal swabs and ceca from samples obtained from hunters. This study involves sampling processed quail carcasses from a large commercial integrated company and also determined the species and antibiotic resistance profile of the Campylobacter isolates. There is considerable debate concerning the ecology of Campylobacter on poultry and perhaps our data can be used by the broiler industry to develop a better understanding of the ecology and to assist in the application of effective intervention strategies. Presently, there is slightly under 100,000 million commercially processed and domestically grown quails in the United States each year, most of which is marketed in Europe as a frozen product. So the presence of an important foodborne pathogen on this food is significant information.

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PHENOTYPIC CHARACTERIZATION OF VIRUlENT AND ANTIMICROBiAL RESISTANT SALMONELLA SPECIES ISOLATED FROM POULTRY

Ibrahim

Ammar

El-dosoky

et al. 2015

Kafrelsheikh Veterinary Medical Journal

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Pathogenesis of Salmonellosis depends upon a large number of factors controlled by an array of genes that synergise into the actual virulence of Salmonella. In the present study, out of 730 samples collected from different poultry farms located in Dakahlia province, Egypt, Salmonella spp were identified in 12%, 8.6% and 3.8% of ducks, chickens and quails respectively. Serological identification showed that the high percentage of isolation was for S. Typhimurium (32.5%) and S. Newport (14%) in chickens, S. Infantis (25%) in ducks and S. Shangani (60%) in quails. Salmonella serovars showed 100% pathogenicity using Embryo lethality assay. The isolated Salmonella serovars were 100% susceptible to only Amikacin and highly resistant to Flumequine, Nalidixic acid, Oxytetracycline, Ampicillin/Sulbctam, Amoxicillin and Trimethoprim-Sulfamethoxazole with the percentages of 90%, 86.7%, 73.3%, 73.3%, 68.3% and 58.3% respectively. The obtained results confirm the need for a country adherence to strict public health and food safety regimes.

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Salmonella spp. in meat-type quails (Coturnix coturnix coturnix) in the State of São Paulo, Brazil

Cited by 3 publications

References 31 publications

Extensive Drug-Resistant Salmonella enterica Isolated From Poultry and Humans: Prevalence and Molecular Determinants Behind the Co-resistance to Ciprofloxacin and Tigecycline

Extensive Drug-Resistant Salmonella enterica Isolated From Poultry and Humans: Prevalence and Molecular Determinants Behind the Co-resistance to Ciprofloxacin and Tigecycline

Incidence, species and antimicrobial resistance of naturally occurring Campylobacter isolates from quail carcasses sampled in a commercial processing facility

PHENOTYPIC CHARACTERIZATION OF VIRUlENT AND ANTIMICROBiAL RESISTANT SALMONELLA SPECIES ISOLATED FROM POULTRY

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