The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

Sernagiotto, Erica Regina; Ducatti, Carlos; Sartori, José Roberto; Stradiotti, Ana Cristina; Maruno, Mariana Kiyomi; Araújo, Priscila Cavalca de; Carvalho, Fabyola Barros de; Pezzato, Antônio Celso

doi:10.1590/s1516-635x2013000100011

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…The range of isotopic values found in serum associated with each diet needs to be defined to allow for effective traceability and the detection of ABPs (Mori et al, 2013;Sernagiotto et al, 2013;Madeira et al, 2014). The δ 13 C and δ 15 N values did not distinguish the experimental groups during the initial period of this study, showing the isotopic similarity of the groups when subjected to the same diet.…”

Section: Discussionmentioning

confidence: 81%

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Silva

Biscola

Santos

et al. 2016

The Veterinary Journal

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Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie - a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for C andN to trace animal proteins in the serum of three groups of sheep: 1 - received only vegetable protein (VP) for 89 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16-89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed N equilibrium 5 days after MBM removal (54th day). Conversely,N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on naturalN signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δC and δN values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.

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Section: Discussionmentioning

confidence: 81%

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Silva

Biscola

Santos

et al. 2016

The Veterinary Journal

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“…Poultry nutrition studies using stable isotopes in diet as markers produce estimates the rate with which tissue stable isotopes are replaced by those present in the diet (Denadai et al, 2007;Gottmann et al, 2008;Pelícia et al, 2011;Araujo et al, 2011;Sernagiotto et al, 2013). Labeled compounds can also be used as biological markers .…”

Section: Incorporation Of Labeled Methionine As a Tissue Tracer In Brmentioning

confidence: 99%

Incorporation of Labeled Methionine as a Tissue Tracer in Broiler Chickens

Stradiotti

Bendassolli²,

Ducatti

et al. 2016

Rev. Bras. Cienc. Avic.

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“…The difference of δ 13 C values between these plants is large enough so that the relative amounts of C 3 and C 4 plants eaten by an animal can be determined from the δ 13 C value of its carbon (DeNiro and Epstein, 1978), enabling analysis of turnover of stable isotopes in animal tissues (Tieszen et al, 1983;Hobson andClarck, 1992, 1993;Hobson, 1995;Hobson and Bairlein, 2003;Ayliffe et al, 2004;Buchheister and Latour, 2010;Oppel and Powell, 2010). More recently, this technique has been used in traceability studies of AOI in poultry feed via tissue analysis (Carrijo et al, 2006;Oliveira et al, 2010;Denadai et al, 2008;Sernagiotto et al, 2013) or identify the origin of a food (Rock, 2012). Furthermore, this technique has been used to evaluate the effect of natural additives, alternative to APE, on the structure of the intestinal mucosa.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope technique

et al. 2018

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A B S T R A C TThe aim of this study was to estimate the metabolic rate of broiler tissues by assessing carbon turnover in different growth phases by using the stable isotope technique and by evaluating the natural changes in Carbon-13 ( 13 C) between C3 and C4 cycle plants. Six-hundred 1-day-old broiler chicks were divided into six groups and subjected to dietary changes containing different isotopic values at 7, 14, 21, 28, and 35 days of age. Birds of Group 1 received diets composed predominantly of C 3 cycle plants shortly after accommodation, while birds of other groups remained predominantly fed with C 4 diet. The diet of Group 2 was mainly replaced by C 3 diet on the 7th day of age. Group 3 started to receive C 3 diet on the 14th day of age, whereas the diet of Group 4 was replaced on the 21st, Group 5 on the 28th. In Group 6, the substitution occurred on the 35th day of age. Samples of the pectoral muscle, keel, tibia, legs muscle, intestinal mucosa, blood, plasma, and feathers were collected. Carbon isotope ratios were determined by mass-spectrometry of isotope ratios. To measure the speed of carbon substitution after a set interval (turnover), a time exponential function was employed and obtained by exponential equations of the first order calculated with Minitab ® 16 software. The carbon half-life varied from 1.78 to 8.20 days for the pectoral muscle, from 1.91 to 12.24 days for the keel, from 2.32 to 10.71 days for the tibia, from 1.87 to 9.43 days for the leg muscle, from 0.8 to 1.58 days for the intestinal mucosa, from 0.64 to 1.71 days for the plasma, from 2.61 to 11.07 days for blood, and from 1.84 to 28.41 days for the feathers during the total growth phase. With the exception of tibia and blood, all other tissues presented a high metabolic rate in the first week of the broilers' life. The metabolic rate of tissues is faster in younger animals, and the metabolism slows down as the broilers get older. The metabolic rates in the pectoral muscle, keel, tibia, and feathers decreased with age, whereas those of the plasma and intestinal mucosa were high in all evaluated periods; thus, these tissues can be used to study traceability of feed at all stages of the broilers' life.

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The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

Cited by 5 publications

References 21 publications

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Incorporation of Labeled Methionine as a Tissue Tracer in Broiler Chickens

Estimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope technique

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