Traceability of animal meals in Japanese quail eggs using the technique of 13C e 15N* stable isotopes

Abstract

The objective of this study was to trace the inclusion of bovine meat and bone meal (BMBM) in the diet of Japanese quails by analyzing eggs and egg fractions (yolk and albumen) by the technique of carbon-13 (13 C) and nitrogen-15 (15 N) stable isotopes. In the trial, 120 Japanese quails were distributed in six treatments with four replicates of five birds each. The following treatments were applied: feed based on corn and soybean meal, containing graded BMBM inclusions (0, 1, 2, 3, 4 or 5%). After 42 days, 20 eggs per treatment were randomly collected for three consecutive days. Ten eggs were used for yolk and albumen sample collection, and ten for total egg sample collection. It was possible to detect the dietary inclusion of 1% BMBM in the egg and its fractions. Therefore, the technique of isotopes 13 C and 15 N is able of tracing since 1% inclusion level of BMBM in the diet of Japanese quails in eggs and their fractions.

Traceability of animal meals in quail eggs using carbon and nitrogen stable isotopes

“…In the albumen samples, the first component caused a greater treatment separation (78.10%) and was more influenced by carbon (0.782) than the second component (21.90%). This 13 C and 15 N isotope enrichment is consistent with the findings of Carrijo et al (2006), Mori et al (2007Mori et al ( , 2008Mori et al ( , 2013, Denadai et al (2008, Oliveira et al (2010), andCruz et al (2012), who used animal meal in the diets of broilers and Japanese quail.…”

“…Previous studies have shown that stable isotopes can detect, via eggs, the inclusion of bovine meat and bone meal in the diets of Japanese quail and in broiler feed (Mori et al, 2007(Mori et al, , 2008(Mori et al, , 2013Oliveira et al, 2010). However, there are no known traceability studies on other animal meals, such as poultry offal meal and feather meal, added to quail diets and assessed through egg analyses.…”

Rastreability of bovine meat and bone meal using the stable isotope technique in egg production

“…The difference between the assessment days was observed in the eggs and its fractions as evidence that the animal is composed of the isotopes it consumes, and exhibits a variation of approximately 2 ‰ for carbon-13 and 3 ‰ for nitrogen-15 (Hobson et al, 2010), which is an outcome that is corroborated by numerous experiments in poultry (Carrijo et al, 2006;Gottmann et al, 2008;Denadai et al, 2009;Oliveira et al, 2010;Cruz et al, 2012;Kanayama et al, 2012 andMóri et al, 2013).…”

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

“…The range of isotopic values found in serum associated with each diet needs to be defined to allow for effective traceability and the detection of ABPs (Mori et al, 2013;Sernagiotto et al, 2013;Madeira et al, 2014). The δ 13 C and δ 15 N values did not distinguish the experimental groups during the initial period of this study, showing the isotopic similarity of the groups when subjected to the same diet.…”