Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain

Abstract

Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates of each serotype were analyzed for the identification of similar and different profiles. Dendrograms were constructed from molecular profiles using the UPGMA method (unweighted pair-group method for the arithmetic averages) and the software program WinBoot. The present study indicates the usefulness of RISA and ARDRA of the 16S-23S rRNA intergenic spacer region (ISR) for systematic, epidemiological, and diagnostic purposes. Since these techniques can be used for the differentiation of serotypes, they are highly promising for the characterization of Salmonella serotypes and intra-serotypes. Data indicate that these techniques may be used to produce more consistent, reliable, and reproducible results in the identification and epidemiological study (traceability) of Salmonella in the poultry industry

Genetic Characterization and Diversity of Rhizobium Isolated From Root Nodules of Mid-Altitude Climbing Bean (Phaseolus vulgaris L.) Varieties

“…The polymerase chain reaction (PCR), electrophoresis and gene sequencing tools have revolutionized the characterization of microorganisms by distinguishing even close members of a species up to the strain level based on distinct genetic trait patterns ( Baginsky et al, 2015 ). Moreover, PCR, metagenomics and third generation gene sequencing methods have higher reliability, faster and more sensitive as compared to other conventional methods ( Baratto et al, 2012 ). In other studies, housekeeping genes such as rec A and atp D, nodulation and symbiotic genes such as nod A and nif H have been recently used to assess rhizobia diversity and phylogeny in different geographic locations due to their high resolution ability ( Ampomah and Huss-Danell, 2016 ; Wang et al, 2016 ).…”

Predominance of Multidrug Resistant Zoonotic <i>Salmonella </i>Enteritidis Genotypes in Poultry of Bangladesh

“…A combined approach of RAPD and ARDRA was done to differentiate genotypes of poultry Salmonella isolates. RAPD-PCR is easy to use and has discriminatory power, and an important tool to fingerprint bacteria involved in disease outbreaks and to determine the sources, vectors and vehicles of transmission (Betancor et al, 2004, Baratto et al, 2012. RAPD typing with 787 primer showed two different profiles (Fig.…”

“…Among the various groups of antibiotics, fluoroquinolones and third-generation cephalosporins are used most frequently for the treatment of Salmonellosis. The earlier drugs Chloramphenicol, Ampicillin, Amoxicillin and Trimethoprim-Sulfamethoxazole are occasionally used as alternatives (Baratto et al, 2012). An increasing rate of antimicrobial resistance in Salmonella has been reported in many developing and developed countries (Uzzau et al, 2000;Mahmud et al, 2011).…”

Comparison of two affordable DNA extraction methods for molecular detection of Salmonella isolates from broiler farm’s boot swabs

“…Molecular typing can be an alternative to identify types of Salmonella spp. (Haddad et al, 2001) due to its rapidness, consistency, reliability and reproducibility (Baratto et al, 2012). PCR methods have been used for the identification of many Salmonella enterica serovars, as Typhimurium, Enteritidis, Gallinarum and Pullorum (Rubio et al, 2019;Lee et al, 2009;Burgarel et al, 2011;Xiong et al, 2017).…”