Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates

Abstract

Although Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine descarxboxylase tests are performed. Therefore, additional tests could be helpful. Many studies have chose the part I of the gene that encodes flagellin (fliC) to differentiate serotypes. Most Salmonella strains have two structural genes (fliC and fliB) that encode flagellins. Non-motile strains generally present these structural genes, but are not able to build a functional flagellum. It was demonstrated that enzymatic restriction of the amplified fliC gene using Hinp1I enzyme can differentiate SG from SP. In the present study, this method was adopted to analyze 14 SP and 22 SG strains, including some strains with atypical results in biochemical tests assessing the utilization of dulcitol and ornithine. The results showed that all SG strains were broken by the enzyme, whereas the 14 SP strains were not.

Isolation and Identification of Salmonella Spp. From Poultry Farms by Using Different Techniques and Evaluation of Their Antimicrobial Susceptibilities

“…Although Salmonella pullorum and Salmonella gallinarum cause different diseases in poultry, they are very similar. Salmonella gallinarum is responsible for fowl typhoid, and Salmonella pullorum causes pullorum disease, both are non-motile and present the same somatic antigenic structure (7). The differentiation between Salmonella pullorum and Salmonella gallinarum is very important from epidemiological and preventive perspectives.…”

“…The differentiation between Salmonella pullorum and Salmonella gallinarum is very important from epidemiological and preventive perspectives. They are very similar, and cannot be distinguished by conventional serological methods (7). Moreover, (22) reported that, the results of serotyping able to reaches only to identification of Salmonella serovar gallinarum without of differentiation of it into their biovars pullorum and gallinarum.…”

“…Bacterial DNA was extracted according to manufacture of bacterial extraction kit (Genaid,korea). Conditions of PCR for rfbsg and rfbsp genes amplification of Salmonella gallinarum and Salmonella pullorum was done according to (8) and for flic according to (7) .…”

“…Most Salmonella strains have structural gene flic that encode flagellins. Non-motile strains generally exhibit these structural genes, but are unable to build up a functional flagellum (7). The primers for allele-specific PCR amplification of rfbsg and rfbsp genes were based on rfbS, these primers used to differentiation of Salmonella gallinarum from Salmonella pullorum (8).…”

Sequence-Level and Dual-Phase Identification of Salmonella Flagellum Antigens by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

“…Different molecular typing methods have been used in attempts to improve the speed, throughput, and quality of Salmonella typing, especially on the basis of flagellar genes. Among the most popular approaches are restriction fragment length polymorphism analysis (9)(10)(11)(12), multiplex PCR (13,14), and DNA microarray (15,16). These approaches are promising in terms of speed and throughput but do not mirror the phenotypic properties and data quality of serotyping.…”