Fowlpox: identification and adoption of prophylactic measures in backyard chickens in Bahia, Brazil

Silva, P. S. da; Batinga, Thaís de Brito; Sales, Tatiane Santana; Herval, Elen Fabiane Guimarães; Ramos, Irma N.; Maia, Paulo César Costa; Fernandes, Lia Muniz Barretto

doi:10.1590/s1516-635x2009000200007

Cited by 12 publications

(24 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Usually the fowlpox is a self-limiting disease and has milder manifestations, as reported by Silva et al (2009) in Brazilian free-range chickens. In the present case, a challenger viral strain might have disseminated to turkey flocks of the Triângulo Mineiro region.…”

Section: Discussionmentioning

confidence: 90%

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

et al. 2018

View full text Add to dashboard Cite

This study describes an outbreak of avian poxvirus disease in previously pox-vaccinated turkeys in Brazil. The turkeys had suggestive gross lesions of cutaneous avian poxvirus in the skin of the head and cervical area without changes in the flock mortality rates. In the slaughterhouse, 30 carcasses were removed from the slaughter line to collect tissue from cutaneous lesions for histological analyses and characterization of the virus. The virus was identified by conventional polymerase chain reaction (PCR) and subsequent gene sequencing. Acanthosis, hyperkeratosis, and hydropic degeneration were seen on skin histopathology. Eosinophilic intracytoplasmic inclusion bodies (Bollinger) on keratinocytes were observed in 46.6% of the samples. Avian poxvirus DNA was detected on PCR in 83.3% of the total samples. PCR associated with histopathology had 93.3% of positivity for avian poxvirus. In the phylogenetic study, samples show 100% matching suggesting that the outbreak occurred by a single viral strain and was different from those strains affecting other wild birds such as canaries and sparrows. A single mutation (Adenine for Guanine) was detected in our study’s strain and in the strains of turkey, chickens, and vaccine strains published in GenBank. Also, when the sequence strain of the present study and sequences from GenBank of canarypox and sparrowpox strains were aligned, a Thymine was found replacing the Adenine or Guanine. The in ovo vaccination method as single-use in turkeys of this study apparently did not provide adequate protection against avianpox disease, but additional vaccination administered by wing-web when turkeys were 45-60 days old in the new flocks controlled the disease. In the subsequent year, new cases of this disease were not found. It was not possible to confirm the source of the virus strain, but infection with a field strain derived from chickens is one possibility, considering the poultry farm population in the area and biosecurity aspects. For wide characterization of avipoxvirus and differentiation among strains, the complete sequence of the viral genome is required.

show abstract

Section: Discussionmentioning

confidence: 90%

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Fallavena et al (2002) reports the presence of FWPV in Dermal Squamous Cell Carcinoma (DSCC) also in broiler chickens using a nested PCR. Silva et al (2009) reports the presence of Fowlpox in backyard chickens by clinical signs and confirming the diagnosis by histopathology. Vargas et al (2011) reported an infection by Avian poxvirus in barn owl, and Pereira et al (2014) reported a cutaneous tumor-like lesions associated with infection by APV in a Dendrocygna autumnalis.…”

Section: Discussionmentioning

confidence: 76%

First phylogenetic analysis of Avipoxvirus (APV) in Brazil

et al. 2016

View full text Add to dashboard Cite

RESUMO.-[Primeira análise filogenética de Avipoxvirus (APV) no Brasil.] Este trabalho representa a primeira aná-lise filogenética de Poxvirus aviário detectado em perus no Brasil. Os distúrbios clínicos relacionados com bouba aviá-ria aqui descritos ocorreram em um sistema de alojamento de perus. As aves apresentaram lesões características de varíola observadas no pescoço, pálpebras e bico das aves. Quatro perus com sinais característicos foram escolhidos aleatoriamente, sacrificados e submetidos à autópsia. This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank ® . The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species. . Estudos adicionais, como isolamento viral, PCR e sequenciamento, incluindo um grande número de perus da produção brasileira devem ser conduzidos devido ao grave risco que a infecção por poxvírus pode causar ao cenário de produção avícola brasileira, tendo em vista que a produção brasileira de perus atrai atenção devido a sua importância mundial. Nossos resultados apontam para a necessidade de identificar a prevalência da APV na produção de peru no Brasil, para realizar estudos de avaliação de risco e continuada monitoração de infecções por APV nas espécies de aves comerciais e silvestres.

show abstract

“…It's used to differentiate FPV from other APVs because it's highly conserved. FPV140 is highly antigenic and immunodominent [1][2][3][4]6,[8][9][10][11][12][13][14][15]17,18].…”

Section: Fpv Related To Genus Avipox (Apvs) Apvs Belong To Subfamilymentioning

confidence: 99%

In silico Prediction of Peptide based Vaccine against Fowlpox Virus (FPV)

St¹,

Salih²,

Basheir³

et al. 2018

Immunome Res

View full text Add to dashboard Cite

Fowlpox: identification and adoption of prophylactic measures in backyard chickens in Bahia, Brazil

Cited by 12 publications

References 7 publications

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

First phylogenetic analysis of Avipoxvirus (APV) in Brazil

In silico Prediction of Peptide based Vaccine against Fowlpox Virus (FPV)

Contact Info

Product

Resources

About