Genomic variants of bacteriophages against Salmonella enterica serovar Enteritidis with potential application in the poultry industry

Robeson, James; Retamales, J.; Borie, César

doi:10.1590/s1516-635x2008000300007

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…For the isolation of lytic bacteriophages against MDR S. enteritidis, 10 sewage water samples were collected, out of which 7 (70%) were found positive for lytic bacteriophages as shown in Table 3. Similar results were reported by Robeson et al (2008) and Khalid et al (2017). All the samples collected from hospitals were found positive for bacteriophages, mainly because of presence of high bacterial contamination as reported (Rasool et al, 2016).…”

Section: Discussionsupporting

confidence: 91%

Efficacy of Lytic Bacteriophages against Multidrug Resistant Salmonella enteritidis from Milk and Meat

Siddique¹

2018

PVJ

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Salmonellosis is major food borne illness caused by Salmonella enteritidis. The emerging multidrug resistance has forced the scientists to investigate alternative control strategies including phage therapy to combat salmonellosis. Therefore, the current study was carried out to isolate multidrug resistance S. enteritidis from raw milk (n=40) and meat (n=30) samples. A total of 10 S. enteritidis isolates were recovered from raw milk whereas 8 isolates were from meat samples. These isolates were confirmed by Polymerase Chain Reaction using spvC gene specific primers yielding 571bp product. The anti-biogram studies were carried out on all isolates using different antimicrobials. The results showed 11 (61%) isolates were susceptible to most of the antibiotics while 7 (39%) isolates were found resistant and hence declared as multidrug resistant S. enteritidis. In the second step, sewage water was processed for the isolation of bacteriophages and was used for bacteriophages enrichment against a susceptible strain of S. enteritidis. Both clear and turbid plaques were isolated and enumerated. The results of bacteriophages isolation showed a total of 7 different bacteriophages isolated out of 10 processed samples. Lytic potential was measured by co-cultivation of S. enteritidis with isolated bacteriophages. The results showed the significant lytic potential of isolated bacteriophages against multidrug resistant S. enteritidis isolates from raw milk and meat samples. Altogether, the current study highlighted the significance of S. enteritidis as an important multi drug resistant food borne pathogen, further the bacteriophages therapy could be an alternative control remedy.

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Section: Discussionsupporting

confidence: 91%

Efficacy of Lytic Bacteriophages against Multidrug Resistant Salmonella enteritidis from Milk and Meat

Siddique¹

2018

PVJ

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“…As expected, these serovars were found in the present study and were submitted to molecular analyses. However, the most prevalent Salmonella serotypes causing human toxic infections are S. Enteritidis and S. Typhimurium (Robeson et al, 2008;Oliveira et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain

Baratto¹,

Gelinski²,

Bernardi³

et al. 2012

Rev. Bras. Cienc. Avic.

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Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates of each serotype were analyzed for the identification of similar and different profiles. Dendrograms were constructed from molecular profiles using the UPGMA method (unweighted pair-group method for the arithmetic averages) and the software program WinBoot. The present study indicates the usefulness of RISA and ARDRA of the 16S-23S rRNA intergenic spacer region (ISR) for systematic, epidemiological, and diagnostic purposes. Since these techniques can be used for the differentiation of serotypes, they are highly promising for the characterization of Salmonella serotypes and intra-serotypes. Data indicate that these techniques may be used to produce more consistent, reliable, and reproducible results in the identification and epidemiological study (traceability) of Salmonella in the poultry industry

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“…All determinations were performed in triplicate. Bacteriophages were titered performing suitable dilutions in phosphate buffer supplemented with 1 mM MgS0 4 and 0.01% gelatin (Robeson et al 2008); 0.1 mL of each phage dilution was mixed with 0.1 mL of an exponentially growing culture of the indicator bacterial strain in 3 mL of molten (45ºC) soft LB agar (0.7%) which was overlayed on LB agar plates supplemented with Rif (100 ug/mL). After incubation for 18-24 hrs at 37ºC bacteriophage plaques that developed were counted.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, egg-white lysozyme has been shown to inhibit the replication of phage M13 by 97-99% in a plaque assay (Sitohy et al 2006). Considering these latter data we sought to test the hypothesis of phage inactivation by experimentally infecting both SPF and commercial eggs with the SE phage f18 (Robeson et al 2008) and testing its stability in the egg milieu.…”

Section: Introductionmentioning

confidence: 99%

Stability inside hen eggs of a Salmonella enterica serovar Enteritidis bacteriophage

Robeson¹,

Valencia²,

Retamales³

et al. 2011

Electro Journal of Biotech

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Background: Bacteriophages are viruses that infect bacteria and therefore are widespread in nature. Those that lyse the pathogen Salmonella enterica serovar Enteritidis (SE) should be expected in niches in which this bacterium thrives, among others the avian egg. Furthermore, bacteriophages could remain viable in this milieu. Results: Upon artificially infecting hen eggs with the SE bacteriophage f18 we found that the bacteriophage titer remains stable at least for up to 144 hrs post-infection , both in yolk and albumen at 25ºC. Conclusion: Bacteriophage f18 withstands the physico-chemical conditions of the egg inner milieu and could be considered for SE-controlling measures in the poultry industry.

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Genomic variants of bacteriophages against Salmonella enterica serovar Enteritidis with potential application in the poultry industry

Cited by 8 publications

References 22 publications

Efficacy of Lytic Bacteriophages against Multidrug Resistant Salmonella enteritidis from Milk and Meat

Efficacy of Lytic Bacteriophages against Multidrug Resistant Salmonella enteritidis from Milk and Meat

Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain

Stability inside hen eggs of a Salmonella enterica serovar Enteritidis bacteriophage

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