Time required to protect the intestinal tract of chicks against Salmonella enterica serovar enteritidis using competitive exclusion

Abstract

Competitive exclusion (CE) has been designed to accelerate the colonization of the alimentary tract of young commercial birds and it has been also used to repopulate the digestive tract after antibiotic therapy. The method has been successfully adopted as a means to prevent enteric salmonellosis. The present study was carried out to evaluate if CE is able to prevent this kind of infection. Newly hatched chicks were given a CE culture and at different intervals of time birds infected with Salmonella Enteritidis were placed together with the group of treated birds. CE culture was prepared from feces of adult laying hens incubated overnight at 37C under aerobic conditions. Birds were killed 4 and 8 days after challenge and viable counts of Salmonella Enteritidis were assessed in the cecal contents. The results showed that Salmonella infection was reduced even if CE culture administration was concomitant with the inclusion of the infected bird in the group

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

“…Sterzo et al, (2005) reported that bacteria present in the hatchery determine the colonization of beneficial or pathogenic bacteria. Cukrowska et al (2002) showed that pathogens can be lodged in the chick´s intestine and colonize in the first contact with microbes in the hatchery, so it is necessary to inoculate probiotic bacteria before the chick and hen can be in contact.…”

Kuluçkadan Çıkış Öncesi ve Sonrası Civcivlerde Probiyotik Suşlarının in ovo Inokulasyonunun Jejunal Goblet Hücre Sayısı ve Morfometrisi Üzerine Etkisi

“…An interesting study demonstrated that the time of initial intestinal colonization by desirable bacteria play an important role on the colonization of pathogens [1] . In the previous studies [4][5][6]8,12] , the protection effects of in ovo inoculation or other route administration of probiotics against Salmonella infection were investigated, but the effects of inoculation of different probiotic strains on intestinal morphometry, and goblet cells count have not been attended.…”

“…In the modern poultry production, the contact between newborn chicks and hens is excluded, and colonization of bacteria in the gut depends on the type of bacteria present in the hatchery environment [1] . This condition exposes chicks to pathogenic bacteria colonization in the gut and causes a delay in desirable bacteria colonization [2] .…”

Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic

“…The use of these bacteria to protect the intestinal tract of chickens against pathogenic microorganisms by competitive exclusion has been studied for years since its first report (Rantala and Nurmi, 1973). Several studies demonstrate nutritional and health benefits, such as increased weight gain and feed intake, better feed conversion and the reduction of Salmonella Enteritidis (SE) burden using Lactobacillusbased probiotics (LBP) (Faber et al, 2012;Fajardo et al, 2012;Haghighi et al, 2008;Higgins et al, 2010;Lima et al, 2007;Molnar et al, 2011;Okamoto et al, 2007;Sterzo et al, 2005). The use of antibiotics in poultry flocks to prevent gastrointestinal infections in commercial chickens is prohibited in many countries and probiotics are suggested as an alternative, especially during the first week of life, when immune responses are limited and chicks are highly susceptible to Salmonella spp.…”