Análise da diversidade genética de cornichão com o uso de marcadores microssatélites

Santos, Armando Martins dos; Agnol, Miguel Dall'; Janke, Aline; Bortolini, Fernanda; Huber, Kátia Graziela Costa

doi:10.1590/s1516-35982011000600005

Cited by 1 publication

(1 citation statement)

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“…Molecular markers make viable to characterization of large number of genotypes with relatively fast and simple procedures (BERED et al, 1997), and have been used to various genetic analysis, as hybrid and clone identification, gene flow studies (FERREIRA; GRATTAPAGLIA, 1998), determination of strategies for the conservation of endangered populations (BUCCI et al, 1997), population genetics studies, paternity tests, forensic science, demographic process, germplasm characterization (KURT et al, 2012;OLIVEIRA et al, 2006;SANTOS et al, 2011), among others.…”

Section: Introductionmentioning

confidence: 99%

In silico analysis of Pinus L. chloroplast DNA to microsatellites regions

Moro¹,

Pereira²,

Filho³

et al. 2019

Sci. For.

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The Pinus genus covers a wide variety of widely cultivated species due to adaptability, high growth and wood quality. Molecular markers have been used for many genetic analyses, and among them, the microsatellite markers (SSR) have several applications and can be found in chloroplast genome (cpDNA) as well as in nuclear genomes (ntDNA). The chloroplast microsatellites markers (cpSSR) can be used for gene flow analysis, identification of hybrids,clones, paternity tests, genetic diversity studies, phylogenetic analysis, among others. The work aimed to characterize the cpSSRs of Pinus species with cpDNA sequenced and deposited in NCBI (National Center for Biotechnology Information). In the twenty species of Pinus spp. studied, 1.542 cpSSRs were identified, with 86,45% of mononucleotide type, and the less frequent the penta-(1.10%) and hexanucleotide (1.04%) types. Predominated cpSSRs in non-coding regions (intergenic). The results indicate presence of a wide range of cpSSR for Pinus spp., which can subsidize breeding programs of interesting species.

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