Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro

Abstract

-The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase α1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase β2 transcripts (0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase β2 genes, however, it affects expression of Na/K-ATPase α1. encontrou-se sobrerregulado, enquanto o gene ATPase-Na/K β2 (0,40 ± 0,30) não diferiu entre os blastocistos produzidos in vitro e aqueles produzidos in vivo. Transcritos para o gene AQP1 não estão presentes em blastocistos bovinos. O sistema de cultivo in vitro não influencia a expressão dos genes AQP3, AQP11 e ATPase-Na/K β2, porém altera a expressão do gene ATPase-Na/K α1.Palavras-chave: blastocele, canal de água, expressão gênica, gradiente osmótico Revista Brasileira de Zootecnia

Effects of Follicular Fluid on Developmental Competence and Gene Expression of in vitro Fertilized Sheep Embryos

“…It has been observed that the embryos produced in vivo are of high quality and less apoptosis than those produced in vitro (Pomar et al, 2005;Melka et al, 2010), and the major reason that might contribute to depress oocyte and embryo competence is the in vitro conditions (Wohlres-Viana et al, 2011). In this study, we have used different concentrations of SFF in an attempt to optimize the in vitro conditions of oocyte maturation to be closer to the in vivo status.…”