Influence of intraruminal infusion of propionic acid and forage to concentrate levels on intake, digestibility and rumen characteristics in young bulls

Ribeiro, Marinaldo Divino; Pereira, José Carlos; Queiróz, Augusto César de; Bettero, Vítor Pereira; Mantovani, Hilário Cuquetto; Silva, Cássio José da

doi:10.1590/s1516-35982009000500023

Cited by 4 publications

(3 citation statements)

References 18 publications

(15 reference statements)

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“…The lower tendency of the pH with supplementation of E. faecium SROD could be related to the production of organic acids by the bacterium. Indeed, a previous study on E. faecium demonstrated that it increased the levels of organic acids such as acetate, propionate, and succinate (Ribeiro et al 2009).…”

Section: Discussionmentioning

confidence: 95%

Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

et al. 2019

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Supplementation of appropriate probiotics can improve the health and productivity of ruminants while mitigating environmental methane production. Hence, this study was conducted to determine the effects of Enterococcus faecium SROD on in vitro rumen fermentation, methane concentration, and microbial population structure. Ruminal samples were collected from ruminally cannulated Holstein–Friesian cattle, and 40:60 rice straw to concentrate ratio was used as substrate. Fresh culture of E. faecium SROD at different inclusion rates (0, 0.1%, 0.5%, and 1.0%) were investigated using in vitro rumen fermentation system. Addition of E. faecium SROD had a significant effect on total gas production with the greatest effect observed with 0.1% supplementation; however, there was no significant influence on pH. Supplementation of 0.1% E. faecium SROD resulted in the highest propionate ( P = 0.005) but the lowest methane concentration ( P = 0.001). In addition, acetate, butyrate, and total VFA concentrations in treatments were comparatively higher than control. Bioinformatics analysis revealed the predominance of the bacterial phyla Bacteroidetes and Firmicutes and the archaeal phylum Euryarchaeota . At the genus level, Prevotella (15–17%) and Methanobrevibacter (96%) dominated the bacterial and archaeal communities of the in vitro rumen fermenta, respectively. Supplementation of 0.1% E. faecium SROD resulted in the highest quantities of total bacteria and Ruminococcus flavefaciens , whereas 1.0% E. faecium SROD resulted in the highest contents of total fungi and Fibrobacter succinogenes. Overall, supplementation of 0.1% E. faecium SROD significantly increased the propionate and total volatile fatty acids concentrations but decreased the methane concentration while changing the microbial community abundance and composition.

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Section: Discussionmentioning

confidence: 95%

Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

et al. 2019

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“…During the dry-off period, cows are typically transitioned to a diet primarily composed of forage with a lower percentage of grain ( Dancy et al, 2019 ), which could impact the metabolic response. For example, propionic acid is a volatile fatty acid produced during rumen fermentation of carbohydrates, and its higher levels may reflect increased fermentation of forage-based diets, particularly grasslage ( Ribeiro et al, 2009 ). Compounds like citrulline, guanidinacetic acid, oxobutanoic acid, riboflavin, myristoyl ethanolamide, and coenzyme Q10 are involved in energy metabolism, amino acid metabolism, and cellular processes, and their levels may be influenced by metabolic shifts.…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolomics and metagenomics reveal signatures for intramammary ceftiofur treatment and lactation stage in the cattle hindgut

Vasco,

Hansen,

Schilmiller

et al. 2024

Front. Mol. Biosci.

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The gut microbiota in cattle is essential for protein, energy, and vitamin production and hence, microbiota perturbations can affect cattle performance. This study evaluated the effect of intramammary (IMM) ceftiofur treatment and lactation stage on the functional gut microbiome and metabolome. Forty dairy cows were enrolled at dry-off. Half received IMM ceftiofur and a non-antibiotic teat sealant containing bismuth subnitrate (cases), while the other half received the teat sealant (controls). Fecal samples were collected before treatment at dry off, during the dry period (weeks 1 and 5) and the first week after calving (week 9). Shotgun metagenomic sequencing was applied to predict microbial metabolic pathways whereas untargeted metabolomics was used identify polar and nonpolar metabolites. Compared to controls, long-term changes were observed in the cows given ceftiofur, including a lower abundance of microbial pathways linked to energy production, amino acid biosynthesis, and other vital molecules. The metabolome of treated cows had elevated levels of stachyose, phosphatidylethanolamine diacylglycerol (PE-DAG), and inosine a week after the IMM ceftiofur application, indicating alterations in microbial fermentation, lipid metabolism, energy, and cellular signaling. Differences were also observed by sampling, with cows in late lactation having more diverse metabolic pathways and a unique metabolome containing higher levels of histamine and histamine-producing bacteria. These data illustrate how IMM ceftiofur treatment can alter the functionality of the hindgut metabolome and microbiome. Understanding how antibiotics and lactation stages, which are each characterized by unique diets and physiology, impact the function of resident microbes is critical to define normal gut function in dairy cattle.

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“…Lactose is a substrate from the genus Enterococcus sp that produces lactate. This is used as an energy source by bacteria of the genus Veilonella sp, which has propionic acid among its end products of the fermentation (Ribeiro et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Effect of whey fermented by Enterococcus faeciumin consortium with Veilonella parvulaon ruminal bacteria in vitro

et al. 2014

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-The objective of this research was to evaluate the effect of whey fermented by Enterococus faecium in consortium with Veilonella parvula in vitro on ruminal microorganisms in different substrates, with or without monensin. The first experiment was carried out in a completely randomized design, in a 6 × 3 factorial arrangement (six substrates × three whey levels) with two replicates. In experiment two, a 2 × 3 × 4 factorial arrangement (with and without monensin, three foods and four levels of fermented whey) was used, in a randomized design with four replicates, totaling 24 treatments. There was no interaction among the wheys and the substrates in the variable for pectin, starch, and carboxymethyl cellulose. There was a greater growth of amylolytic and pectinolytic microorganisms and a lower growth of proteolytic and cellulolytic microorganisms. A significant effect of optical density was found in the media without substrate and that containing trypticase and glucose due to the addition of fermented whey. There was interaction for the pH at 24 hours among whey, food and monensin. For ammonia at 24 hours there was effect for food, whey and monensin, and interaction among factors. For microbial protein at 24 hours, there was effect for food, whey, monensin and no interaction among sources of variation. The use of whey fermented by bacteria Enterococcus faecium and Veilonella parvula improves microbial protein synthesis by ruminal bacteria in media containing different energy sources. The combination of fermented whey and monensin shows variable results in relation to microbial growth.

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Influence of intraruminal infusion of propionic acid and forage to concentrate levels on intake, digestibility and rumen characteristics in young bulls

Cited by 4 publications

References 18 publications

Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

Untargeted metabolomics and metagenomics reveal signatures for intramammary ceftiofur treatment and lactation stage in the cattle hindgut

Effect of whey fermented by Enterococcus faeciumin consortium with Veilonella parvulaon ruminal bacteria in vitro

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