Diminuição do teor de óxido de crômio (III) usado como marcador externo

Bremer‐Neto, Hermann; Graner, Celso Augusto Fessel; Pezzato, Luiz Edivaldo; Padovani, Carlos Roberto; Cantelmo, Osmar Angelo

doi:10.1590/s1516-35982003000200001

Cited by 28 publications

(11 citation statements)

References 24 publications

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“…The following components of the feces were analyzed: crude protein, gross energy, fat, and chromium oxide. The analyses of the diets and feces followed the methodology described by Horwitz (2005), except for the chromium analysis that followed the protocol described by Bremer Neto et al (2003).…”

Section: Methodsmentioning

confidence: 99%

Fish protein hydrolysate in diets for Nile tilapia post-larvae

Silva

Rocha

Moreira

et al. 2017

Pesq. agropec. bras.

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-The objective of this work was to determine the apparent digestibility coefficient (ADC) of crude protein, crude energy, fat, and dry matter of fish protein hydrolysate (FPH), made of by-products of Nile tilapia (Oreochromis niloticus) and whole sardines (Cetengraulis edentulus), and to evaluate the productive performance and muscle fiber growth of Nile tilapia post-larvae. Two trials were conducted, the first one to determine the digestibility in 120 fingerlings (70.0±2.0 g), and the second one to evaluate the productive performance of 375 post-larvae, with three days of age, which were distributed in 25 aquaria with 30 L of useful volume. Five diets were prepared based on vegetable ingredients, to which fish were included at 0, 2, 4, 6, and 8% FPH. For the evaluation of muscle growth, eight fish of each experimental unit were used. The ADC values found were: 98.29% for dry matter; 99.28% for crude protein; and 99.13% for gross energy. The best zootechnical response for the productive performance resulted from the treatment with the inclusion of fish hydrolysate at 4.75%. The diets affected the frequency of the muscle fiber diameters, mainly the growth by hyperplasia. FPH can be efficiently used, and its inclusion at 4.75% is indicated in the diets for Nile tilapia in the post-larvae stage.Index terms: Cetengraulis edentulus, Oreochromis niloticus, digestibility, fish meal, nutrition, peptides, pisciculture. Hidrolisado proteico de peixe em dietas para pós-larvas de tilápia-do-niloResumo -O objetivo deste trabalho foi determinar o coeficiente de digestibilidade aparente (CDA) da proteína bruta, da energia bruta, da gordura e da matéria seca de um hidrolisado proteico de peixe (HPP), feito de resíduos de tilápia-do-nilo (Oreochromis niloticus) e sardinha inteira (Cetengraulis edentulus), e avaliar o desempenho produtivo e o crescimento das fibras musculares de pós-larvas de tilápia-do-nilo. Realizaram-se dois ensaios, o primeiro para a determinação da digestibilidade em 120 alevinos (70,0±2,0 g) e, o segundo, para avaliação do desempenho produtivo de 375 pós-larvas, com três dias de idade, distribuídas em 25 aquários (unidade experimental) com volume útil de 30 L. Cinco rações à base de ingredientes vegetais foram elaboradas, às quais se incluíram os peixes a 0, 2, 4, 6 e 8% de HPP. Para a avaliação do crescimento muscular, oito peixes de cada unidade experimental foram utilizados. Os valores de CDA encontrados foram: 98,29% para matéria seca; 99,28% para proteína bruta; e 99,13% para energia bruta. As melhores respostas zootécnicas quanto ao desempenho produtivo resultaram do tratamento com a inclusão do hidrolisado proteico a 4,75%. As dietas influenciaram a frequência do diâmetro das fibras musculares, principalmente o crescimento por hiperplasia. O HPP pode ser eficientemente utilizado, e sua inclusão a 4,75% é indicada em dietas de tilápia-do-nilo na fase de pós-larva.Termos para indexação: Cetengraulis edentulus, Oreochromis niloticus, digestibilidade, farinha de peixe, nutrição, peptídeos, pisci...

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Section: Methodsmentioning

confidence: 99%

Fish protein hydrolysate in diets for Nile tilapia post-larvae

Silva

Rocha

Moreira

et al. 2017

Pesq. agropec. bras.

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“…Gross energy, crude protein, and dry matter of feces were analyzed by the same methods used to analyze the test ingredients and diets. The concentration of chromic oxide in the diets and feces was analyzed following the method proposed by Bremer Neto et al (2003). Total amino acids in the diets and feces were analyzed by the hydrolysis with hydrochloric acid 6 N for 24 h. After the acid hydrolysis, the free amino acids reacted with phenyl isothyocianate and separated by high performance liquid chromatography in reverse phase and detected by UV light at 254 nm.…”

Section: Methodsmentioning

confidence: 99%

Digestibility of Alternative Protein‐Rich Feedstuffs for Channel Catfish, Ictalurus punctatus

Kitagima¹,

Fracalossi²

2011

J World Aquaculture Soc

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Apparent digestibility coefficients (ADCs) of amino acids, crude protein, gross energy, and dry matter of canola meal, corn gluten feed, fish offal meal, shrimp and fish offal meal, poultry by‐product meal, and hydrolyzed feather meal were determined for channel catfish, Ictalurus punctatus. Experimental diets contained 30% test ingredient, 69.5% casein‐based reference diet, and 0.5% chromic oxide. Groups of 20 fish (102.45 g) were fed the experimental diets twice a day at 3.5% body weight. Fecal samples were collected in triplicate daily at 0000 h and 0600 h after settlement into collection devices. Shrimp and fish offal meal and corn gluten feed presented significantly lower (P < 0.05) ADC for dry matter (59.5 and 39.3%) suggesting their low value as feedstuffs for catfish feeds. Although ADC values for crude protein were above 80% for all the test ingredients, amino acid digestibility varied significantly, except for histidine whose ADC remained constant regardless of the protein source. Lysine was the first limiting amino acid in most of the test ingredients, except in fish offal meal and poultry by‐product meal. Among the protein sources tested, only fish offal meal and poultry by‐product meal met channel catfish amino acid requirements for a 28% digestible protein grow‐out diet.

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“…After the collection period, the feces were dried in forced ventilation oven at 55°C for 72h and it was carried out physical and chemical analysis (crude protein, ether extract, dry matter, mineral matter and energy) as well as the feed and the test ingredient, according to Association of Official Analytical Chemists (AOAC, 2000). It was also held chromium oxide analysis of feces and diets, according to the methodology described by Bremer Neto, Graner, Pezzato, Padovani, and Cantelmo (2003).…”

Section: Digestibility Evaluationmentioning

confidence: 99%

<b>Tilapia by-product hydrolysate powder in diets for Nile tilapia larvae

Sary

Paris

Bernardi

et al. 2017

Acta Sci. Anim. Sci.

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ABSTRACT. This study aimed to evaluate the digestibility of tilapia by-product protein hydrolysate powder (TBHP) from tilapia filleting by-product, and its inclusion in diets for Nile tilapia larvae. In order to determine the apparent digestibility coefficients, two diets were formulated, the reference diet and the test diet (20% of TBHP) and, to evaluate the inclusion, six diets were formulated, which were with 0.0, 2.0; 4.0; 6.0, and 8.0% of TBHP. The apparent digestibility coefficients of crude protein and gross energy were 89.5 and 98.3%, respectively. At levels above 4.0% of inclusion of TBHP, the weight, final length and weight gain were impaired, however, survival rate was higher (p < 0.05). The TBHP can be used in diets for tilapia due to the high digestibility coefficients for crude protein and gross energy. The inclusion of up to 4.0% of TBHP does not affect the reproductive performance and survival rate of Nile tilapia larvae.Keywords: digestibility, fish nutrition, performance.Hidrolisado proteico seco do resíduo da filetagem de tilápias em dietas para larvas de tilápia do Nilo RESUMO. Objetivou-se avaliar a digestibilidade do hidrolisado proteico seco do resíduo da filetagem de tilápias (HPST) e sua inclusão em dietas para larvas de tilápia do Nilo. Para determinar os coeficientes de digestibilidade aparente, duas dietas foram elaboradas, referência e teste (com 20% do HPST) e para avaliação da inclusão, seis dietas foram formuladas com 0,0, 2,0; 4,0; 6,0, e 8,0% de HPST. Os coeficientes de digestibilidade aparente da proteína e energia bruta foram de 89,5 e 98,3%, respectivamente. Nos níveis acima de 4,0% de inclusão de HPST o peso e comprimento final e ganho em peso foram prejudicados, no entanto a sobrevivência foi maior (p < 0,05). O HPST pode ser utilizado em dietas para tilápias em função dos elevados coeficientes de digestibilidade para proteína bruta e energia bruta. A inclusão de até 4,0% de HPST não afeta o desempenho produtivo e a sobrevivência de larvas de tilápias do Nilo.Palavras-chave: digestibilidade, nutrição de peixes, desempenho.

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Diminuição do teor de óxido de crômio (III) usado como marcador externo

Cited by 28 publications

References 24 publications

Fish protein hydrolysate in diets for Nile tilapia post-larvae

Fish protein hydrolysate in diets for Nile tilapia post-larvae

Digestibility of Alternative Protein‐Rich Feedstuffs for Channel Catfish, Ictalurus punctatus

<b>Tilapia by-product hydrolysate powder in diets for Nile tilapia larvae

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