Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation

Ruiz, Azulamara da Silva; Chauffaille, Maria de Lourdes Ferrari; Alves, Solivanda Trindade; Oliveira, José Salvador Rodrigues de

doi:10.1590/s1516-31802009000500002

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Large number of alleles along with its high allelic heterozygosity among individuals in population has made D1S80 a useful genetic marker in population genetics study (Herrera et al, 2004;Verbenko et al, 2003). D1S80 locus provides more information about a population compared to other VNTRs and STRs (Lauritzen and Mazumder, 2008;Ruiz et al, 2009). The number of effective D1S80 alleles (Ne) was the highest (15.313) in the Monipuri population and the least (8.817) in Khasias (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Genetic polymorphism study at D1S80 VNTR in four major tribal populations (Garo, Santal, Khasia and Monipuri) of Bangladesh

Sajib

Hasan

et al. 2016

Asian Australas. J. Biosci. Biotechnol.

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D1S80 is a highly informative variable number of tandem repeat (VNTR) marker located in the telomeric region of chromosome 1 at 1p36-p35. Since its discovery, the D1S80 locus has been used widely in determining the origins and genetic relations among and between various populations worldwide. Here, we studied the allele frequencies and other population genetic parameters at the D1S80 locus among individuals from four major tribal populations in Bangladesh (Santal, Garo, Monipuri and Khasia). The data was then compared with the other populations including the mainstream Bengali population. A total of 31 different alleles were detected with repeat unit numbers ranging between 14 and 50. D1S80 allele with 18 repeats was the most frequent in three populations except the Santals, in which allele with 19 repeats was the most common. Observed heterozygosity was less than the expected in all four populations. Pair-wise observed genetic distances were, in general, more between the tribal populations and the mixed mainstream Bangladeshis compared to the distances between the tribal populations. Comparison of D1S80 allelic frequency distribution among seventy-six different populations placed the tribal populations along with the mainstream mixed Bangladeshis, Tamils and mixed Panjabi Indians in a clade separated from the rest. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (2), 386-393

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Section: Resultsmentioning

confidence: 99%

Genetic polymorphism study at D1S80 VNTR in four major tribal populations (Garo, Santal, Khasia and Monipuri) of Bangladesh

Sajib

Hasan

et al. 2016

Asian Australas. J. Biosci. Biotechnol.

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Chimerism Testing

Baxter‐Lowe¹

2016

Manual of Molecular and Clinical Laboratory Immunology

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Study of whole blood and T cell chimerism in monitoring HLA identical allogeneic hematopoietic transplantation

Deghady¹,

Elsorady²,

Lewis³

et al. 2018

HTIJ

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Chimerism analysis is an important method for monitoring outcome of allogenic hematopoietic stem cell transplantation. Variable number tandem (VNTR) analysis is considered an informative technique to follow up chimerism state. The aim of the work is to study certain number of VNTRs to identify their potential value in the detection of chimerism in transplanted patients in conventional ablative transplants. Also to demonstrate the value of T cells subset analysis in detecting transplantation outcomes. This study included 17 pairs undergoing HSCT. Informative loci pre-transplantation using five VNTR loci and two gene loci were identified. After transplantation the informative loci were used to detect chimerism status. After DNA extraction from blood samples, amplification of VNTR loci was performed using a conventional PCR protocol. Extra sample post transplantation was collected and pre-treated with resetting technique in order to separate T cells, its product was subjected to DNA extraction then amplification of informative loci was done. Amplified product of DNA samples was run on 2% agarose gel stained using ethidium bromide. Fourteen recipients showed full done chimerism in both whole blood and T cells separated cells, one recipient died after HSCT, one recipient showed split chimerism and one pair failed to detect informative locus. VNTR analysis using a panel of five loci is suitable to detect state of chimerism after HSCT.

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Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation

Abstract: These methods were sensitive and informative. In VNTR, the association of APO-B with D1S80 showed 88% informativeness. The quantitative FISH method was more sensitive, but had the disadvantage of only being used for sex-unmatched pairs.

Cited by 3 publications

References 25 publications

Genetic polymorphism study at D1S80 VNTR in four major tribal populations (Garo, Santal, Khasia and Monipuri) of Bangladesh

Genetic polymorphism study at D1S80 VNTR in four major tribal populations (Garo, Santal, Khasia and Monipuri) of Bangladesh

Chimerism Testing

Study of whole blood and T cell chimerism in monitoring HLA identical allogeneic hematopoietic transplantation

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