Is automated platelet counting still a problem in thrombocytopenic blood?

Oliveira, Rita de Cássia da Silva; Takadachi, Maria Mariko; Nonoyama, Kimiyo; Barretto, Orlando César de Oliveira

doi:10.1590/s1516-31802003000100005

Cited by 12 publications

(9 citation statements)

References 15 publications

(17 reference statements)

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“…All these lead to inaccurate platelet counts in an automated hematology analyzer [8][9][10][11] . Hence platelet count values obtained from automated analyzers should be crosschecked by some other method particularly in cases of thrombocytopenia [12] .…”

Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

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Background: Automated hematology analyzers have a low degree of accuracy in predicting platelet counts in patients with thrombocytopenia. Hence automated platelet counts should be crosschecked by reviewing the peripheral blood smear in cases of thrombocytopenia. However existing methods for platelet count estimation from peripheral blood smears have certain drawbacks. Hence we suggest a new method to estimate platelet count from peripheral smear and compared it with the existing methods. Conclusion:Platelet count estimation based on platelet/RBC ratio in ten oil immersion fields and total RBC count is a simple and reliable method to estimate platelet counts from peripheral smears.

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Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

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“…Mean platelet volumes were measured separately on 150 L of whole blood obtained by retro-orbital puncture and diluted with 150 L 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) on the Advia 2120 (Bayer Diagnostics, Tarrytown, NY), which optimized measurement of platelet volume. [23][24][25] Platelet half-life studies Platelets were isolated from animals that were either wild-type (WT) for PF4 or overexpressed hPF4. Briefly, animals were anesthetized, blood was isolated from the portal vein, and 600 L of blood was placed in acid-citrate-dextrose (ACD) buffer and centrifuged at 200g for 10 minutes at room temperature to prepare platelet-rich plasma (PRP).…”

Section: Platelet Counting and Sizementioning

confidence: 99%

Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications

Lambert

Rauova

Bailey

et al. 2007

Blood

109

115

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Platelet factor 4 (PF4) is a negative regulator of megakaryopoiesis in vitro. We have now examined whether PF4 regulates megakaryopoiesis in vivo by studying PF4 knockout mice and transgenic mice that overexpress human (h) PF4. Steady-state platelet count and thrombocrit in these animals was inversely related to platelet PF4 content. Growth of megakaryocyte colonies was also inversely related to platelet PF4 content. Function-blocking anti-PF4 antibody reversed this inhibition of megakaryocyte colony growth, indicating the importance of local PF4 released from developing megakaryocytes. The effect of megakaryocyte damage and release of PF4 on 5-fluorouracil-induced marrow failure was then examined. Severity of thrombocytopenia and time to recovery of platelet counts were inversely related to initial PF4 content. Recovery was faster and more extensive, especially in PF4-overexpressing mice, after treatment with anti-PF4 blocking antibodies, suggesting a means to limit the duration of such a chemotherapy-induced thrombocytopenia, especially in individuals with high endogenous levels of PF4. We found that approximately 8% of 250 healthy adults have elevated (> 2 times average) platelet PF4 content. These individuals with high levels of platelet PF4 may be especially sensitive to developing thrombocytopenia after bone marrow injury and may benefit from approaches that block the effects of released PF4. IntroductionMegakaryopoiesis is a complex process that is still not fully understood. Early studies identified thrombopoietin (TPO) as the predominant cytokine responsible for regulating platelet counts. However, many other cytokines have been postulated to participate in regulating megakaryopoiesis by increasing TPO expression in the liver (eg,), enhancing megakaryocyte chemotaxis (eg, stromal-derived factor-1 [SDF-1]), 1 or directly stimulating megakaryocyte development (eg, IL-11). 2 A pathway by which megakaryopoiesis is auto-down-regulated has been suggested based on in vitro studies of platelet factor 4 (PF4) and later by studies of other chemokines that are also stored in ␣-granules, including the related CXC chemokines, neutrophil activating peptide-2 (NAP-2), and 3,4 and the more distantly related CC chemokines, RANTES (regulated upon activation, normal T-cell-expressed and secreted), 5 and MIP-1␣ (macrophage inflammatory peptide-1␣). 4,5 PF4 is a 7.8-kDa protein that is produced primarily in megakaryocytes and expressed in platelets as a tetramer, where it comprises a significant portion of the content of ␣-granules (2.5% on a molar basis). 6 The biological role of PF4 is not fully understood. Unlike other chemokines that have clearly defined chemokine receptors, PF4 appears to function by binding with high affinity to glycosaminoglycans on cell surfaces. 7-9 PF4 has been proposed to participate in many important biological process based primarily on in vitro studies, including roles in angiogenesis, 10 inflammation, 11 atherosclerosis, 12,13 thrombosis, [14][15][16][17] and megakaryopoiesis. 4,5,18 Studies...

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“…The hematology analyzer platelet count ranged from 5 to 55x10 4 platelets per µl. The PBS platelet count estimation method showed mean (20.25x10 4 platelets per µl) and median (18x10 4 platelets per µl) when compared to hematology analyzer platelet count method (mean 19.13x10 4 platelets per µl and median 16.5x10 4 platelets per µl respectively). The standard deviations of platelet count in the whole blood by hematology analyzer method and PBS method were 9.4062x10 4 and 9.2084x10 4 platelets per µl respectively.…”

Section: Resultsmentioning

confidence: 89%

“…Neubauer's counting chamber), PBS examination, immunoplatelet counting and radioisotope labelling technique. 3,4 of PBS (where RBC's do not overlap each other) with 20x10 9 /L. The platelet count results obtained by this method of PBS examination had no statistically significant difference when compared with hematology analyzer platelet count results for same blood samples.…”

Section: Introductionmentioning

confidence: 74%

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Utility of peripheral blood smear in platelet count estimation

Anchinmane¹,

Sankhe²

2019

Int J Res Med Sci

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Background: There are several methods of platelet count used in hematology laboratory. These methods are manual counting, automated hematology analyzer counting, platelet count estimation by peripheral blood smear (PBS) method etc. Many diseases such as dengue, malaria, pregnancy induced hypertension etc. may leads to severe thrombocytopenia. Timely and precise diagnosis of platelet count plays very crucial role in critical care management of thrombocytopenia cases. The present study was undertaken to estimate platelet counts by PBS method and correlate them with results from automated hematology analyzer method.Methods: Study included one hundred randomly collected blood samples in EDTA anticoagulant vacutainer tubes. Each blood sample was processed for platelet count estimation with automated hematology analyzer and Leishman’s stained PBS examination. The statistical analysis was done by using Pearson's correlation test to access the agreement between both the methods.Results: The Pearson's correlation test showed significant positive correlation for platelet count estimation between both the methods. (r =0.9789).Conclusions: Platelet count estimation by PBS method is reliable and statistically significant when compared to hematology analyzer method. PBS platelet estimation method can be taken as early and rapid procedure for platelet assessment in critical severe thrombocytopenia cases. This method is simple, cheaper and can be done in rural hospital setup where automation is not available.

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Is automated platelet counting still a problem in thrombocytopenic blood?

Cited by 12 publications

References 15 publications

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications

Utility of peripheral blood smear in platelet count estimation

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