Detection of human papillomavirus in epithelial lesions of the conjunctiva

Palazzi, Maristela Amaral; Erwenne, Clélia Maria; Villa, Luisa L.

doi:10.1590/s1516-31802000000500003

Cited by 42 publications

(26 citation statements)

References 24 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HPV16 was detected in 11% of our OSSN cases with a positivity of 9% in SCC patients and 15% in dysplastic cases. Although earlier studies have also demonstrated HPV16 in OSSN,6 23 others have demonstrated HPV18 as well as multiple genotypes in their cases 8 9. However, multiple high-risk-HPV types are reported more commonly in cervical neoplasia 24…”

Section: Discussionmentioning

confidence: 94%

Human papillomavirus: a predictor of better survival in ocular surface squamous neoplasia patients

et al. 2012

View full text Add to dashboard Cite

Background Although human papillomavirus (HPV) has been implicated in the pathogenesis of ocular surface squamous neoplasia (OSSN), no study has so far dealt with the prognostic role of HPV. In this study the presence and significance of HPV in OSSN and its correlation with p16INK4a immunoexpression was determined. Methods HPV was detected by HPV-L1 capsid genespecific multiplex PCR using PGMY09/11 primers, and genotyping was done by linear array on 64 OSSN patients and 15 conjunctival controls. p16INK4a immunoexpression as a marker for HPV presence was also evaluated. Results The HPV genome was detected in 11% of cases by multiplex PCR, and all positives belonged to a high-risk HPV16 genotype. p16INK4a Overexpression was seen in 28% (18/64) of cases. Control conjunctival tissues were negative for HPV and p16INK4a expression. The presence of HPV was associated with significantly improved disease-free survival ( p=0.02) as well as p16INK4a overexpression ( p=0.001). The sensitivity and specificity of p16INK4a as a marker for HPV presence was 86% and 79%, respectively, with a positive predictive value of 33% and a negative predictive value of 98%. Conclusions The results of this study point towards HPV as a predictor of better survival in a subset of HPV-positive OSSN patients. Although p16 INK4a immunoexpression is a useful indicator of HPV presence in OSSN, confirmation by multiplex PCR is necessary.

show abstract

Section: Discussionmentioning

confidence: 94%

Human papillomavirus: a predictor of better survival in ocular surface squamous neoplasia patients

et al. 2012

View full text Add to dashboard Cite

show abstract

“…A frequência de detecção do HPV nas displasias intraepiteliais da superfície ocular varia consideravelmente, dependendo da metodologia utilizada (7,(10)(11)(12)(14)(15)(16)(17)(18) . A captura híbrida parece ser o método mais empregado, detectando os 18 principais tipos do vírus, incluindo os que causam lesão de alto grau e carcinoma (8,19,20) .…”

Section: Discussionunclassified

“…Alguns trabalhos na literatura demonstraram a presença de HPV na conjuntiva através de outros métodos de detecção, como reação em cadeia da polimerase (PCR) e a hibridização por dot blot (7) . Porém, tais métodos possuem custo elevado e não são rotineiramente empregados no país, restringindo-se a uso para pesquisa.…”

Section: Discussionunclassified

See 1 more Smart Citation

Captura híbrida negativa em displasia intraepitelial isolada de córnea

Martin

Filho

Odashiro³

et al. 2010

Rev. bras.oftalmol.

View full text Add to dashboard Cite

RESUMOAs displasias intraepiteliais de córnea correspondem a lesões de baixo risco de malignidade, dentro do espectro das neoplasias intraepiteliais da superfície ocular. Essas displasias se apresentam como áreas leucoplásicas e têm como um dos principais fatores de risco o papilomavírus humano (HPV). No presente trabalho, os autores descrevem um caso de displasia intraepitelial isolada de córnea, tratada através de excisão cirúrgica, com confirmação histológica e resultado negativo de hibridização de DNA para HPV.

show abstract

“…Possui etiologia multifatorial (2,3) ida de avançada (4) , exposição a raios ultravioleta (4,5) , e a derivados do petróleo, fumaça de cigarro, infecção pelo papilomavírus humano (HPV) (5) , e pelo vírus da imunodeficiência humana (HIV). O CEC conjuntival possui baixo grau de malignidade, facilmente identificável, raramente leva à metástase e responde muito bem à ex cisão cirúrgica; entretanto, pode haver recorrência, o que o torna mais agressivo (6) .…”

Section: Introductionunclassified

Carcinoma espinocelular de conjuntiva com evolução para exenteração: relato de caso

Satto

Marques²,

Schellini³

2012

Arq. Bras. Oftalmol.

View full text Add to dashboard Cite

show abstract

Detection of human papillomavirus in epithelial lesions of the conjunctiva

Abstract: The low frequency of HPV DNA found in the lesions of this sample and the detection of the viral genome in the normal mucosa indicate that there is a weak possibility of association between HPV infection and the carcinoma of the conjunctiva.

Cited by 42 publications

References 24 publications

Human papillomavirus: a predictor of better survival in ocular surface squamous neoplasia patients

Human papillomavirus: a predictor of better survival in ocular surface squamous neoplasia patients

Captura híbrida negativa em displasia intraepitelial isolada de córnea

Carcinoma espinocelular de conjuntiva com evolução para exenteração: relato de caso

Contact Info

Product

Resources

About