Correlation between endometrial dating of luteal phase days 6 and 10 of the same menstrual cycle

Abstract

CONTEXT: Endometrial maturation, important in the diagnosis of infertile couples, has been evaluated since 1950 using the Noyes criteria. Nevertheless, there is no consensus regarding the most suitable period of the luteal phase for performing the biopsy. OBJETIVE: This study evaluated the correlation between the histological dating of two endometrial biopsies performed in the same menstrual cycle, on luteal phase days six and ten. DESIGN: Prospective study. SETTING: Human Reproduction Division of the Federal University of São Paulo, referral center. PATIENTS:Twenty-five women complaining of infertility had their menstrual cycles monitored by ultrasound and LH plasma levels, to obtain evidence of ovulation. PROCEDURES: Endometrial biopsies were performed on luteal phase days LH+6 and LH+10 (luteal phase day 1 = LH+1 = the day that follows LH peak). Dating was done according to morphometric criteria, in which an endometrium sample is considered out of phase if the minimum maturation delay is one day. On day LH+6, blood was drawn for plasma progesterone level determination. RESULTS: All patients had an ovulatory cycle (mean LH peak: 47.4 U/L; mean follicular diameter on LH peak day: 18.9 mm; mean endometrial thickness on LH peak day: 10.3 mm; mean plasma progesterone level on day LH+6: 14.4 ng/ml). 14 patients had both biopsies in phase; 5 patients had out of phase biopsies only on day LH+6; 3 had out of phase biopsies only on day LH+10 and 3 patients had out of phase biopsies on both days. McNemar's test showed no statistical difference between these data (p>33.36%). CONCLUSIONS: The correlation found between the endometrial datings suggests that biopsies performed on either of these two days are suitable for evaluation of endometrial maturation.

Non-invasive prediction of implantation window in controlled hyperstimulation cycles: Can the time from the menstrual day at embryo transfer to expected menstrual cycle give a clue?

“…Study assessed the histological features of endometrium both at the 6 th day after luteinising hormone (LH) surge and the 10 days after LH surge. Study revealed similar histological features with regard to endometrial maturation ( 28 ) , in another study, pinopodes were observed at 20th day of menstruation and indicated period of implantation window started to open at days of 22-23 in women with 28 day regular cycles ( 29 ) , as we mentioned above there is two to four days maturation advancement in stimulated cycles. Our data also showed some advancement in endometrial maturation but more sooner embryo transfers failed to implant.…”

Molecular Basis of Endometriosis and Endometrial Cancer: Current Knowledge and Future Perspectives

“…However, understanding of the regenerative mechanism of the endometrium during the menstrual cycle and the location of endometrial progenitor/stem cells have not been fully elucidated [10][11][12]. The traditional morphological theory of the endometrium describes it as two-dimensional (2D) histological structure [13][14][15]. However, due to the complexity of the morphology of the endometrial glands, the technical characteristics of 2D histopathological imaging have been found to be insufficient [4].…”