Results of local excision in rectal cancer: is there good evidence?

Matos, Délcio

doi:10.1590/s1516-31801997000500003

Cited by 2 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A sample of each phase was re-dissolved in ethanol and qualitative tests for tannins (gelatin and ferric chloride) and flavonoids (Shinoda) were performed. [ 12 13 ] Phases that tested positive were further evaluated for their antimicrobial activity and cytotoxicity. Where antimicrobial activity was detected, it followed a further separation by column chromatography with the purpose of structure elucidation and identification of compounds present.…”

Section: Methodsmentioning

confidence: 99%

Bioassay-guided In vitro study of the antimicrobial and cytotoxic properties of the leaves from Excoecaria lucida Sw

Ochoa-Pacheco¹,

Arranz²,

Beaven³

et al. 2017

Phcog Res

View full text Add to dashboard Cite

Background:Excoecaria lucida Sw. (Euphorbiaceae) is a plant conventionally used throughout the Caribbean in the treatment of infectious diseases.Objective:To evaluate, using bioassay-guided fractionation, the in vitro cytotoxicity and antimicrobial activity of E. lucida leaves.Materials and Methods:A 95% ethanol crude extract was dried and fractionated by solid-liquid separation in four phases (hexane, dichloromethane, ethyl acetate, and butanol). Antimicrobial activity (3 bacteria, 6 yeasts, and 2 fungi) was evaluated by the dilution method with resazurin (2048, 512, 128, 32, and 8 μg/mL). The cytotoxicity assays were evaluated in two cell lines: MRC-5 and RAW 264.7; calculating the selectivity index. Assays were performed for the total extract, the isolated compound with the highest yield, and the ethyl acetate and butanol phases. Isolated compounds were characterized by nuclear magnetic resonance and mass spectrometry techniques.Results:Fractionation process led to the isolation of ellagic acid (784.29 mg), 3,3',4'-tri-O-methyl ellagic 4-O-β-D-glucopyranoside acid (6.1 mg), and corilagin (6.91 mg). The most active were ethyl acetate phase and ellagic acid with IC50= 128 μg/mL against seven and five different species of microorganisms, respectively. The total extract (IC50=512 μg/mL) and the ethyl acetate phase (IC50=128 μg/mL) were cytotoxic in both cell lines, while butanol phase and ellagic acid both with IC50>2048 μg/mL seemed to be safer.Conclusions:The results obtained indicate that the Excoecaria leaves can be conventionally used as antimicrobial, but it should be present that some cytotoxicity could appear. In addition, the three identified compounds were reported for the first time in the species.SUMMARYExcoecaria lucida leaves (Euphorbiaceae) are used by the Cuban population due to their antimicrobial activity. This ethnopharmacological knowledge is confirmed by the integrated antibacterial and antifungal in vitro screening developed, using the bioassay-guided fractionation method.Abbreviations Used: MRC-5-SV2: Diploid human lung fibroblasts cells, RAW 264.7: Murine macrophages cells, IC50: Inhibitory Concentration 50%, ATCC: American Type Culture Collection, CCEBI: Culture Collection of Industrial Biotechnology Center, CECT: Spanish Culture Collection Type, CFU: Colony forming units, CC50: 50% cytotoxic concentration, CO2: Carbon dioxide, SI: Selectivity index, IR: Infrared spectroscopy, 1H NMR: Nuclear Magnetic Resonance of hydrogen, 13C NMR: Nuclear Magnetic Resonance of carbon, HMQC: Heteronuclear Multiple-Quantum Correlation, HMBC: Heteronuclear Multiple Bond Correlation, COSY: Correlation Spectroscopy, NOESY: Nuclear Overhauser Effect Spectroscopy, KBr: Potassium bromide, DMSO-D6: Deuterated dimethyl sulfoxide, LC.MS: Liquid Chromatography-Mass Spectrometry, [α]D: Optical rotation, EL1: ellagic acid, EL2: 3,3’,4’-tri-O-methyl ellagic 4-O-β-D-glucopyranoside acid, EL3: corilagin, Active (+), inactive (-).

show abstract

Section: Methodsmentioning

confidence: 99%

Bioassay-guided In vitro study of the antimicrobial and cytotoxic properties of the leaves from Excoecaria lucida Sw

Ochoa-Pacheco¹,

Arranz²,

Beaven³

et al. 2017

Phcog Res

View full text Add to dashboard Cite

show abstract

“…Here, attention was given to the classes of secondary metabolism substances, for which material conditions were available (existence of reagents) for their identification, in this case, Flavonoids, Tannins, Alkaloids, Saponins, Steroids, Triterpenes, Anthraquinones, and Phenols. The procedures used are those recommended by [9,10,11,3,12,13].…”

Section: Identification Of Compounds Present In Grewia Flavescens Roo...mentioning

confidence: 99%

“…The presence of these groups in the Grewia flavescens root extract was checked according to the criterion used by [9], according to which the observation of the expected characteristic reaction indicates the presence of the result.…”

Section: Identification Of Compounds Present In Grewia Flavescens Roo...mentioning

confidence: 99%

Bioactive potentialities of Grewia flavescens root extract in the treatment of alcohol dependence: Preliminary phytochemical study

Sousa Ferrão,

Germano Janeque

2024

PDD

View full text Add to dashboard Cite

Although the use of plants for medicinal purposes is an ancient practice, there is a perception that in the field of chemical dependency, its role has been and is little studied. With regard to alcohol dependence, the third leading cause of hospitalization in psychiatric hospitals in Mozambique, an ethnobotanical work was carried out in 2018 in the Municipality of Massinga, province of Inhambane, with the aim of identifying with practitioners of traditional medicine, the plants they use in their treatment, having resulted, among other plants, in the identification of Grewia flavescens, from which the need arose, based on phytochemical prospecting and literary framework support, to carry out a study of a preliminary nature on the bioactive potential of the root extract (part indicated in the survey) of Grewia flavescens in the treatment of alcohol dependence. The results showed the presence of Flavonoids, Tannins, Alkaloids, Triterpenoids and Saponins. About this group of bioactives, there is bibliographical evidence that points to the anxiolytic and anti-depressant action of Flavonoids, Saponins and Alkaloids, allowing to infer that these can act as mood stabilizers in patients in the phase of alcohol withdrawal syndrome and craving during treatment, not despite deepening from preclinical studies.

show abstract

Results of local excision in rectal cancer: is there good evidence?

Cited by 2 publications

References 1 publication

Bioassay-guided In vitro study of the antimicrobial and cytotoxic properties of the leaves from Excoecaria lucida Sw

Bioassay-guided In vitro study of the antimicrobial and cytotoxic properties of the leaves from Excoecaria lucida Sw

Bioactive potentialities of Grewia flavescens root extract in the treatment of alcohol dependence: Preliminary phytochemical study

Contact Info

Product

Resources

About