Direct immunofluorescence in Lupus Erythematosus (LE)

Isfer, Roseli Svartman; Sanches, José Antônio; Neto, Cyro Festa; Cernea, Selma Schuartz; Oliveira, Zilda Najjar Prado de; Vilela, Maria Apparecida Constantino; Silva, Maria Luíza; Miyachi, Lucy Massaco; Fukumori, Lígia M. I.; Silva, Soraia Regina da

doi:10.1590/s1516-31801996000200007

Cited by 9 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous research showed that the detection rate of immunoreactants in lesional skin varied from 30% to 50% and that IgM was the most frequent immunoreactant [12], which is consistent with other published data [3], [13]–[15]. We enrolled 64 patients diagnosed with SLE and examined DIF conducted on lesional skin to assess whether the type and number of cutaneous immunoreactants present in the lesional skin correlated with serological disorders and disease severity as measured by the SLEDAI.…”

Section: Introductionsupporting

confidence: 85%

Correlation of Cutaneous Immunoreactants in Lesional Skin with the Serological Disorders and Disease Activity of Systemic Lupus Erythematosus

Luo

Tan

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Detection of immunoreactants including IgG, IgM, IgA, and C3 by direct immunofluorescence (DIF) from skin is useful for distinguishing lupus lesions from other skin disorders. Despite their diagnostic value, the type and number of cutaneous immunoreactants as they relate to serological disorders and disease severity has been poorly studied. We examined 36 patients with systemic lupus erythematosis (SLE) with positive DIF (DIF+) and 28 patients with negative DIF (DIF−) tests performed on lesional skin. Among DIF+ patients, the most frequent patterns of immunoreactants were IgM alone (36%) and the coexistence of IgM with C3 (28%). IgM was the highest detected individual immunoreactant (86%). As classified by number, 17 of 36 DIF+ patients had one immunoreactant ( = 1), while the remaining patients had two to four immunoreactants (>1). Compared with DIF− patients, DIF+ patients were more likely to have severe disease as indicated by lower serum C3 levels and a higher SLE disease activity index (SLEDAI). The coexistence of IgM with any other immunoreactants indicated a more severe disease than that present in the DIF− group, whereas the IgM-alone group was comparable with the DIF− group in both serum C3 levels and SLEDAI. These findings were also applicable in the comparison of patients with more than one (>1) immunoreactant and patients with no (DIF−) and one ( = 1) immunoreactant. Collectively, the presence of multiple immunoreactants in lesional skin implies a more severe disease activity of SLE, while a single immunoreactant may be equal to the absence of immunoreactants (DIF−) in terms of predicting disease activity.

show abstract

Section: Introductionsupporting

confidence: 85%

Correlation of Cutaneous Immunoreactants in Lesional Skin with the Serological Disorders and Disease Activity of Systemic Lupus Erythematosus

Luo

Tan

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The diagnosis of KFD was performed by lymph node biopsy, but some discrepancies with this diagnosis were seen from serologic parameters such as positive antinuclear antibody, dsDNA, extractable nuclear antigen, SSA/Ro, and anti‐straight muscle antibodies.. The DIF showed the association of IgM+ C3 microgranular deposits at the epidermal–dermal junction (in accordance with the literature), typical DIF of lupus erythematosus and cutaneous lupus erythematosus 10 …”

Section: Discussionsupporting

confidence: 68%

“…Our patient reported an episode of malar rash and erythema at the age of 20 years old, then three years ago she developed a lupus-like panniculitis and itchy (a) (b) Figure 1 Erythematous scleroatrophic skin lesions first appeared on the superior, external part of the arms, and on the buttock the association of IgM+ C3 microgranular deposits at the epidermal-dermal junction (in accordance with the literature), typical DIF of lupus erythematosus and cutaneous lupus erythematosus. 10 As the diagnosis of KFD is mainly based on histopathological studies of the affected lymph nodes (to better define the patient's skin lesions), we performed an immunohistochemical and EM study on lesional skin.…”

Section: Discussionmentioning

confidence: 99%

Why is Kikuchi–Fujimoto disease misleading?

et al. 2011

View full text Add to dashboard Cite

show abstract

“…[11] The second most common deposit at the DEJ was IgM, which tended to exhibit a strong intensity in association with LE. [15][16][17] Considering all the results, we suggest that any immunoreactant deposit at CB plus fibrinogen deposition, whether alone or combined with other immunoreactants at DEJ, favors LP. Meanwhile, the deposition of any immunoreactant at CB plus IgG or intense IgM at DEJ, favors LE.…”

Section: Discussionmentioning

confidence: 75%

Diagnostic significance of colloid body deposition in direct immunofluorescence

Chularojanamontri¹,

Tuchinda²,

Triwongwaranat³

et al. 2010

Indian J Dermatol Venereol Leprol

View full text Add to dashboard Cite

Immunoreactant deposits at CB alone can be found in various diseases but a strong intensity and high quantity favor the diagnosis of interface dermatitis. CB plus dermoepidermal junction (DEJ) deposits are more common in interface dermatitis than any other disease. Between lichen planus (LP) and discoid lupus erythematosus (DLE), CB alone is more common in LP; whereas, CB plus DEJ and superficial blood vessel (SBV) is more common in DLE. The most common pattern in both diseases is CB plus DEJ. The quantity and intensity of CB in LP is higher than in DLE.

show abstract

Direct immunofluorescence in Lupus Erythematosus (LE)

Cited by 9 publications

References 14 publications

Correlation of Cutaneous Immunoreactants in Lesional Skin with the Serological Disorders and Disease Activity of Systemic Lupus Erythematosus

Correlation of Cutaneous Immunoreactants in Lesional Skin with the Serological Disorders and Disease Activity of Systemic Lupus Erythematosus

Why is Kikuchi–Fujimoto disease misleading?

Diagnostic significance of colloid body deposition in direct immunofluorescence

Contact Info

Product

Resources

About