In vitro study of cytotoxicity of orthodontic elastomeric ligatures

Santos, Rogério Lacerda dos; Pithon, Matheus Melo; Freire, Paulyana Pryscilla de Melo; Romanos, Maria Teresa Villela

doi:10.1590/s1516-14392012005000068

Cited by 3 publications

(2 citation statements)

References 31 publications

(44 reference statements)

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“…Numerous studies have been performed to evaluate the in vitro cytotoxicity of orthodontic elastic materials, including bands, separators, and ligatures [9][10][11][12][13]. Those reports have shown that latex elastomeric materials show cytotoxicity in vitro, typically using a cell viability test.…”

Section: Introductionmentioning

confidence: 99%

“…According to ISO 10993-12, the extraction conditions should attempt to simulate the clinical use conditions to determine the potential toxicological hazard without causing significant changes. However, to date, the in vitro cytotoxicity tests on latex rubber bands reported in the orthodontic literature have been performed without a serial dilution of the extracts and without any stretching or compressing of the materials [9,11,13,18], even though the latex rubber bands are used in contact with oral mucosa and saliva in a stretched state of up to three times their length ("3L") [17]. Moreover, mouse fibroblast (L929) cells have typically been used to evaluate the cytotoxicity of latex elastomeric materials, which are less reflective of the response of human oral cells against the harmful extracts from the latex rubber bands.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity Comparison of the Nanoparticles Deposited on Latex Rubber Bands between the Original and Stretched State

Lee

Kwon

et al. 2014

Journal of Nanomaterials

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Understanding the biocompatibility of nanoparticles in dental materials is essential for their safe usage in the oral cavity. In this study, we investigated whether nanoparticles deposited on orthodontic latex rubber bands are involved in the induction of cytotoxicity. A method of stretching to three times (“3L”) the length of the latex rubber bands was employed to detach the particles using the original length (“L”) for comparison. The cytotoxicity tests were performed on extracts with mouse fibroblasts (L929) and human gingival fibroblasts (HGFs). Fourier transform infrared spectroscopy, ion chromatography, elemental analysis, and inductively coupled plasma mass spectrometry (ICP-MS) were performed to detect the harmful components in the extracts from rubber bands. There was a significant decrease in the cell viability in the “L” samples compared with the “3L” samples (P<0.05) in the L929 and HGF cells. This was due to the Ni single crystal nanoparticles (~50nm) from the inner surface of “L” samples that were detached in the “3L” samples as well as the Zn ion (~9 ppm) detected in the extract. This study revealed that the Ni nanoparticles, as well as Zn ions, were involved in the induction of cytotoxicity from the latex rubber bands.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Comparison of the Nanoparticles Deposited on Latex Rubber Bands between the Original and Stretched State

Lee

Kwon

et al. 2014

Journal of Nanomaterials

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Corrosion of metal orthodontic brackets and archwires caused by fluoride-containing products: Cytotoxicity, metal ion release and surface roughness

2018

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Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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The aim of this study is to evaluate cytotoxicity between polyurethane and non-latex orthodontic elastomeric ligatures. Seven elastomeric ligatures of black-coloured from different manufactures (3 non-latex, 3 polyurethane and 1 latex) were divided into 7 groups of 10 elastics each: Group UK (nonlatex, 3M Unitek), Group TP (nonlatex, TP Orthodontics), Group AO (nonlatex, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident) and Group TC (latexControl, TP Orthodontics). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with 1-way analysis followed by the Tukey post hoc test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05). There was a statistically differences (p<.05) between the viability cell of Groups UK, TP and AO with the Groups O, M, TD and TC at 1, 2, 3 and 7 days. There was no statistically differences (p>.05) between the groups O, M and TD at in all experimental times. It can be concluded that the nonlatex elastomeric ligatures of Unitek, TP Orthodontics, and American Orthodontics showed higher cell viability compared to others ligatures.

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In vitro study of cytotoxicity of orthodontic elastomeric ligatures

Cited by 3 publications

References 31 publications

Cytotoxicity Comparison of the Nanoparticles Deposited on Latex Rubber Bands between the Original and Stretched State

Cytotoxicity Comparison of the Nanoparticles Deposited on Latex Rubber Bands between the Original and Stretched State

Corrosion of metal orthodontic brackets and archwires caused by fluoride-containing products: Cytotoxicity, metal ion release and surface roughness

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

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