Evaluation of blood compatibility of plasma deposited heparin-like films and SF6 plasma treated surfaces

Abstract

In devices used in open-heart surgery and dialysis, blood must be continuously processed using extracorporeal circuits composed of peristaltic pumps and active components such as specific filters and oxygenators. Several procedures have been employed to avoid blood coagulation induced by contact with the artificial surfaces of such devices. Often heparin, a bioactive protein able to prevent clot formation, is employed. In this work, we have used heparin-containing gas plasmas to evaluate the possibility of depositing adherent anticoagulant films onto PVC and glass surfaces. The films were produced by radiofrequency plasma enhanced chemical vapor deposition from heparin/isopropanol and heparin/hexamethyldisiloxane solutions. In addition, the effects of exposure to SF 6 plasmas on the compatibility of such surfaces have also been investigated. The blood compatibility was evaluated through the determination of the density of platelets and fibrinogen and activated partial thromboplastin (APTT) and prothrombin times (PT) of human blood freshly collected and after contact for 2.5 hours with different surfaces. The deposited films were also characterized by infrared spectroscopy, contact angle and surface energy measurements. The coagulation time of blood, placed in contact with glass substrates coated by PECVD films of heparin/isopropanol mixtures, and in contact with SF 6 plasma-treated PVC, increased by about 60 and 20%, respectively, compared to the values measured with untreated samples.

Grafting of zwitterion from polysulfone membrane via surface-initiated ATRP with enhanced antifouling property and biocompatibility

“…Furthermore, to improve antifouling property and biocompatibility of membranes, several polymers were used for surface modification over the past two decades, such as heparin [15][16][17][18][19], heparin-like polymers [20][21][22][23][24], PEG [6,8,9,25,26], phospholipid polymers [3][4][5][27][28][29] and zwitterionic polymers [7,[30][31][32][33][34][35][36][37][38][39][40], and so on. It was reported that the polymers with zwitterionic structures (sulfobetaine and carboxybetaine) could maintain normal conformation of biomacromolecules and improve biocompatibility [30,34].…”

Catechol Chemistry Inspired Approach to Construct Self-Cross-Linked Polymer Nanolayers as Versatile Biointerfaces

“…The ultimate goal for the surface design or modification of biointerfaces is to endow them with specific or favourable properties, such as antifouling performance, anti-thrombogenic ability, promotion of cell attachment, and superior antibacterial ability, thereby making them competent in biomedical fields. [7][8][9] To achieve specific designed functional biointerfaces, multiple methods have been developed, such as physical blending, 10 chemical grafting, 11 surface plasma treatment, 12 layer-by-layer (LbL) assembly, 13 and etc.. Among these methods, blend of functional polymers or nano/micromaterials usually decreases the mechanical property of the bulk matrix and lacks generality for various applications.…”

Heparin-like surface modification of polyethersulfone membrane and its biocompatibility

“…However, the immobilized heparin density was very small in the earlier studies. In order to solve this problem, Perrenoud et al [8] prepared heparin-deposited PVC film by plasma treatment, and it was illustrated that the plasma technique could enhance the density of the immobilizing sites for heparin and other biological molecules. Nevertheless, there are still some inconveniences for these heparin-immobilization methods, which restrict the application of heparin-modified materials.…”