Antimicrobial activity of different root canal filling pastes used in deciduous teeth

Abstract

The purpose of this study was to evaluate in vitro the antimicrobial activity of materials used as root canal filling in deciduous teeth by direct exposure test. The materials tested were: Guedes-Pinto, CTZ, zinc oxide eugenol, Calen  , L&C and MTA pastes. A microbial mixture was prepared with 1 mL each of pure suspension of the following microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans. For the direct exposure test, sterilized paper points were contaminated with the microbial mixture suspension and placed in contact with the respective materials for 24, 48 and 72 hours. At these intervals, paper points were removed and immersed in 7 mL of Letheen broth, followed by incubation at 37 °C/48 hours. 100 µL of inoculum obtained from Letheen broth was transferred to 7 mL of BHI under identical incubation conditions. Microbial growth was evaluated by macroscopic examination of BHI broth turbidity. In conclusion, only the CTZ and MTA pastes showed antimicrobial activity

<p>Investigating The Antimicrobial Activity Of Different Root Canal Filling Pastes In Deciduous Teeth</p>

“…Unlike our research, in a study carried out by Fabiane et al22, it was concluded that MTA had high levels of antimicrobial activity at different times, and no antibacterial activity was observed for ZOE and two calcium hydroxide-based products including Caien and L&C direct exposure test was used. Ramar et al23 in a study depicted that Endofloss compared with Metapex and ZOE was a better material in the treatment of deciduous teeth.…”

Root canal filling materials in primary teeth - review

“…9,15,16 Considering the limitations of the root canal instrumentation and the obstacles to the intracanal irrigants, the use of root canal filling material with antimicrobial activity is of great importance for the success of the endodontic treatment in primary dentition. 10,13 Numerous researchers investigated the antimicrobial spectrum of action of the materials used and the results obtained demonstrated significant divergence. [17][18][19][20] The agar diffusion test has been widely used for this purpose.…”

“…It is the first recommended obturating material for primary teeth and is still the most commonly and traditionally used material in the USA for treatment of endoinfections in deciduous teeth and the second choice in Brazilia. 10,21 The disadvantages of this material include a slower rate of resorption than the physiological one of the root of the primary teeth, absence of biocompatibility, transitory to severe irritation of the periapical tissues, necrosis of the alveolar bone and cementum, but it does not show cytotoxicity. 10,23,24 Antoniazzi et al showed a risk of causing a deviation of eruption trajectory of the permanent successor tooth if the paste was forced through the radicular apex.…”

“…10,21 The disadvantages of this material include a slower rate of resorption than the physiological one of the root of the primary teeth, absence of biocompatibility, transitory to severe irritation of the periapical tissues, necrosis of the alveolar bone and cementum, but it does not show cytotoxicity. 10,23,24 Antoniazzi et al showed a risk of causing a deviation of eruption trajectory of the permanent successor tooth if the paste was forced through the radicular apex. 3 The same authors also found more rapid absorption of ZOE when mixed with blood, fluids, and debris.…”

“…8 The complex anatomy of the root canal system (sharp curvatures and lateral canals) and the physiological resorption (no apical limit, occurs irregularly, not seen radiographically oftentimes) make the access and the instrumentation hard. 3,9,10 In addition, Faria et al found that several resistant microorganisms could persist after biomechanical preparation and use of intracanal irrigants. 11 Observing the importance of this aspect of the endodontic infections in primary dentition, a significant number of researchers emphasized the antimicrobial activity of the root canal filling material.…”