Teste in vitro de citotoxicidade: estudo comparativo entre duas metodologias

Rogero, Sizue Ota; Lugão, Ademar B.; Ikeda, Tamiko Ichikawa; Cruz, Ana M.

doi:10.1590/s1516-14392003000300003

Cited by 140 publications

(136 citation statements)

References 4 publications

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“…In order to verify the lysosomal viability after the period of 24 hours, a technique known as cytotoxicity assay for the neutral red retention was performed, according to Borenfreund and Puerner (1985) and Rogero et al (2003). The cell culture medium with water sample was then discarded and DMEM (without serum) containing 50 µg of neutral red/mL was added.…”

Section: Neutral Red (Nr) Testmentioning

confidence: 99%

“…Among alternative methodologies of environmental monitoring is the use of cell lines, which may be applied in several genotoxicity and cytotoxicity assays due to their easy manipulation and sensitivity when exposed to chemical and physical agents, in addition to presenting good reproducibility (Rogero et al, 2003;Cardozo et al, 2006;Morales, 2008;Žegura et al, 2009). Metabolically competent cell lines are among the best lines used to detect and evaluate the risks that chemical compounds can pose to human health, since they are able to reflect the metabolism of xenobiotic compounds better than other in vitro models, which demand exogenous metabolic activation (Gábelová et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

et al. 2015

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Some water bodies in the Sinos River Basin (SRB) have been suffering the effects of pollution by residential, industrial and agroindustrial wastewater. The presence of cytotoxic and genotoxic compounds could compromise the water quality and the balance of these ecosystems. In this context, the research aimed to evaluate the genotoxicity and cytotoxicity of the water at four sites along the SRB (in the cities of Santo Antônio da Patrulha, Parobé, Campo Bom and Esteio), using bioassays in fish and cell culture. Samples of surface water were collected and evaluated in vitro using the Astyanax jacuhiensis fish species (micronucleus test and comet assay) and the Vero lineage of cells (comet assay and cytotoxicity tests, neutral red -NR and tetrazolium MTT). The micronucleus test in fish showed no significant differences between the sampling sites, and neither did the comet assay and the MTT and NR tests in Vero cells. The comet assay showed an increase in genetic damage in the fish exposed to water samples collected in the middle and lower sections of the basin (Parobé, Campo Bom and Esteio) when compared to the upper section of the basin (Santo Antônio da Patrulha). The results indicate contamination by genotoxic substances starting in the middle section of the SRB.Keywords: water quality, micronucleus test, comet assay, fish bioassays, cell culture. Avaliação da genotoxicidade e da citotoxicidade de amostras de água da Bacia do Rio dos Sinos, sul do Brasil ResumoAlguns corpos d'água da Bacia Hidrográfica do Rio dos Sinos (BHRS) vêm sofrendo os efeitos da poluição por efluentes domésticos, industriais e agroindustriais. A presença de compostos citotóxicos e genotóxicos pode comprometer a qualidade da água e o equilíbrio desses ecossistemas. Neste contexto, o objetivo do trabalho foi avaliar a genotoxicidade e a citotoxicidade da água em quatro pontos ao longo da BHRS (Santo Antônio da Patrulha, Parobé, Campo Bom e Esteio), utilizando bioensaios em peixes e em cultura celular. As amostras de água de superfície foram coletadas e avaliadas in vitro utilizando a espécie de peixe Astyanax jacuhiensis (teste de micronúcleo e ensaio cometa) e a linhagem celular tipo Vero (ensaio cometa e os testes de citotoxicidade vermelho neutro -VN e tetrazólio MTT). O teste de micronúcleos em peixes não apresentou diferenças significativas entre os pontos de coleta, assim como o ensaio cometa e os testes VN e MTT nas células Vero. O ensaio cometa demonstrou aumento nos danos genéticos em peixes expostos às amostras de água coletadas nos trechos médio e inferior da bacia (Parobé, Campo Bom e Esteio) em relação ao trecho superior da bacia (Santo Antônio da Patrulha). Os resultados indicam contaminação por substâncias genotóxicas a partir do trecho médio da BHRS.Palavras-chave: qualidade da água, teste de micronúcleo, ensaio cometa, bioensaios com peixes, cultura celular.

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Section: Neutral Red (Nr) Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

et al. 2015

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“…Latex fragments were used as positive control and confirmedly non-toxic filter paper discs as negative control, respecting the dimension of the superficial area. The plates were analyzed macroscopically and microscopically using the halo presence and the cell integrity around the sample as parameters, respectively 15 .…”

Section: Cytotoxicity Evaluation Using the Agar Diffusion Methodsmentioning

confidence: 99%

“…This technique uses cell culture and the toxicity can be measured quantitatively by cell lysis (cell death), inhibition of cell growth and other effects caused by the devices, materials and extracts. The cells used to make cultures are acquired from previously established lines and supplied by resellers of biological products or cell and tissue banks, such as the American Tissue Culture Collection (ATCC) due to the high reproducibility, efficiency and availability [14][15] . The objective of this study was to obtain nanostructured membranes of Chitosan/Peo using the electrospinning technique.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity tests for nanostructured chitosan/PEO membranes using the agar diffusion method

et al. 2012

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Electrospinning is used to produce fibers in the nanometer range by stretching a polymeric jet using electric fields of high magnitude. Chitosan is an abundant natural polymer that can be used to obtain biocompatible nanostructured membranes. The objectives of this work were to obtain nanostructured membranes based on blends of chitosan and polyoxyethylene (PEO), and evaluate their thermal and morphological properties, as well as their in vitro biocompatibility by agar diffusion cytotoxicity tests for three different cell lines. A nanostructured fibrous membrane with fiber diameters in the order of 200 nm was obtained, which presented a rough surface and thickness ranging from one to two millimeters. The results of the cytotoxicity tests evidenced that the chitosan/PEO membranes are non-toxic to the cells studied in this work. Further, the electrospinning technique was effective in obtaining nanostructured chitosan/PEO membranes, which showed biocompatibility according to in vitro preliminary tests using the cell lines.

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“…É fundamental ressaltar que a promoção de permeação de fár-macos pela pele através da onda sônica pode levar a maior eficácia da liberação de ativos; aumento do fluxo ou retenção do mesmo, aumento da liberação localizada; tópica ou dos tecidos alvo através da pele; ou, ainda, a combinação das hipóteses citadas. No entanto, esta liberação poderá promover efeitos tóxicos tanto intracelulares como extracelulares, caso não haja utilização adequada da quantidade do composto associado, 18 ou, ainda, devido à ocorrência de alteração da estrutura química do composto. 5 Poderão ocorrer efeitos adversos e não desejáveis ao tratamento, assim como a utilização adequada poderá promover redução de possíveis agentes tóxicos ao organismo.…”

Section: Introductionunclassified

Avaliação dos efeitos do ultrassom terapêutico sobre a cafeína e verificação da liberação em sistema de difusão vertical

Tassinary¹,

Bianchetti²,

Rempel³

et al. 2011

Quím. Nova

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Teste in vitro de citotoxicidade: estudo comparativo entre duas metodologias

Cited by 140 publications

References 4 publications

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

Cytotoxicity tests for nanostructured chitosan/PEO membranes using the agar diffusion method

Avaliação dos efeitos do ultrassom terapêutico sobre a cafeína e verificação da liberação em sistema de difusão vertical

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