Synthesis of polyHEMA hydrogels for using as biomaterials. Bulk and solution radical-initiated polymerization techniques

Seidel, Juliana Matos; Malmonge, Sônia Maria

doi:10.1590/s1516-14392000000300006

Cited by 47 publications

(30 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These MCSs can be prepared in regular nonadhesive culture dishes, siliconcoated dishes [88], containers coated with nonadhesive enhanced polymers (such as PDMS [89]) or poly-HEMA [90], agarose gels [91], alginate beads [92], spinner flasks [78], or hanging drop cultures [93]. After reaggregation of dissociated cells, each MCS has the capacity to fuse with other MCSs.…”

Section: Preparation Of Multicellular Spheroidsmentioning

confidence: 99%

In Vitro Biofabrication of Tissues and Organs

Nakayama

2013

Biofabrication

View full text Add to dashboard Cite

Section: Preparation Of Multicellular Spheroidsmentioning

confidence: 99%

In Vitro Biofabrication of Tissues and Organs

Nakayama

2013

Biofabrication

View full text Add to dashboard Cite

“…PolyHEMA is a hydrogel that has biomedical potential due to its ability to form a three-dimensional, hydrophilic polymeric network, with a soft and rubbery consistency, low interfacial tension, which closely resembles the ECM environment (Kudela, 1976; Lombello et al, 2002; Seidel and Malmonge, 2000). However, the high degree of hydophilicity and non-ionic nature of PolyHEMA are factors that contribute to a poor substrate for binding secreted ECM proteins that are positively charged; thereby, decreasing the ability of cells to adhere to PolyHEMA coated surfaces.…”

Section: Introductionmentioning

confidence: 99%

LG4–5 domains of laminin‐211 binds α‐Dystroglycan to allow myotube attachment and prevent anoikis

Muñoz

Zhou

Jarrett

2009

Journal Cellular Physiology

View full text Add to dashboard Cite

Poly(2-hydroxyethyl methacrylate) (PolyHEMA) prevents cell attachment was used here to study anoikis, the process where cells die when unattached or attached to an inappropriate matrix, in mouse C 2 C 12 myotubes. A method was developed to efficiently embed proteins into PolyHEMA and the effect on cultured myotubes was determined. Myotubes grown on PolyHEMA-coated plates fail to attach to the surface and remain as rounded, suspended cells, undergo dramatic increases in apoptosis and necrosis, and the number of viable cells decreases,. Incorporation of merosin (laminin-211) or the short laminin globular (LG4-5) modules of the laminin α2 chain C-terminus (called 2E3) that binds α-dystroglycan diminishes both apoptosis and necrosis and increases viability while bovine serum albumin had a much lesser effect, showing the specificity of this effect for these matrix proteins. One sarcolemma receptor for laminin-binding is α-dystroglycan. An antibody which binds α-dystroglycan but which does not block laminin-binding (VIA4) had little effect on apoptosis or viability on merosin or 2E3 embedded plates while another antibody (IIH6) which specifically blocks binding dramatically decreased viability and increased apoptosis. When merosin or 2E3 are added to culture media rather than embedded on plates these can also increase viability and decrease apoptosis even though the cells remain in suspension, though the effect is not as great as found for the embedded proteins where the cells attach. Thus, we conclude that the binding of a small LG4-5 modules of laminin-211 to α-dystroglycan is important in preventing anoikis and that attachment plus binding is necessary for maximal cell survival.

show abstract

“…However, the situation is complicated by the fact that the T g s of the glassy hydrophilic blocks are likely to drop upon exposure to water vapor. 26 Irrespective, the lower mobility of the glassy blocks may lead to an inability of the PHEMA-and PAA-containing copolymers to reorganize which will prevent delamination.…”

Section: Resultsmentioning

confidence: 99%

Surface-Induced Selective Delamination of Amphiphilic ABA Block Copolymer Thin Films

et al. 2004

View full text Add to dashboard Cite

We demonstrate the unforeseen property of selective adhesion/delamination of amphiphilic block copolymer films in coating hydrophobic and hydrophilic substrates. When spin-coated from THF solutions onto hydrophilic substrates (e.g., glass and O 3-treated silicon wafer), amphiphilic poly-(oligoethylene glycol methyl ether methacrylate) (POEGMA)-based ABA block copolymers 1 and 2 formed thin films with hydrophobic surfaces. Upon exposure to water, these films undergo a fast rearrangement to a hydrophilic surface before they delaminate from the substrate. In contrast, when deposited on a hydrophobic substrate (e.g., Au, Si, Ag), the same copolymer films do not undergo any surface rearrangement and remain as coherent thin films on the substrate. From contact angle measurements it becomes clear that the delamination is accompanied by a rapid surface rearrangement from a hydrophobic to hydrophilic nature. This rearrangement is not observed for the copolymer over hydrophobic surfaces despite the identical constitutions of the copolymers and identical microphase-separated surface morphologies. It is shown that within the range of polymers investigated this behavior was only observed for POEGMA-containing triblock copolymers. Moreover, it is also shown that in order to show this delamination behavior the polar A-block must be large compared to the apolar B-block. On the basis of XPS and AFM data, we propose that this selective adhesion/delamination is a direct consequence of the self-organization of the block copolymers in the polymer thin films.

show abstract

Synthesis of polyHEMA hydrogels for using as biomaterials. Bulk and solution radical-initiated polymerization techniques

Cited by 47 publications

References 9 publications

In Vitro Biofabrication of Tissues and Organs

In Vitro Biofabrication of Tissues and Organs

LG4–5 domains of laminin‐211 binds α‐Dystroglycan to allow myotube attachment and prevent anoikis

Surface-Induced Selective Delamination of Amphiphilic ABA Block Copolymer Thin Films

Contact Info

Product

Resources

About