Antimicrobial activity of the essential oil of Cymbopogon citratus (DC) Stapf. on Staphylococcus spp., Streptococcus mutans and Candida spp.

Almeida, R. T.; Akisue, Gokithi; Cardoso, Lml; Junqueira, J.C.; Jorge, AO C

doi:10.1590/s1516-05722013000400002

Cited by 19 publications

(16 citation statements)

References 35 publications

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“…14 A recent study 15 showed the antimicrobial activity of Cymbopogon citratus (Poaceae) extract on standard strains and clinical streptococci isolates. Additionally, previous studies 16,17,18 tested the activity of C. citratus essential oil on Streptococcus mutans monospecies biofilm. These studies pointed to the promising potential of this natural substance for application in anti-caries products.…”

Section: Introductionmentioning

confidence: 99%

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Oliveira¹,

Borges²,

Brighenti

et al. 2017

Braz. oral res.

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The objective of this study was to evaluate the effects of Cymbopogon citratus essential oil and its main compound (citral) against primary dental colonizers and caries-related species. Chemical characterization of the essential oil was performed by gas chromatography/mass spectroscopy (GC/MS), and the main compound was determined. Antimicrobial activity was tested against Actinomyces naeslundii, Lactobacillus acidophilus, S. gordonii, S. mitis, S. mutans, S. sanguinis and S. sobrinus. Minimum inhibitory and bactericide concentrations were determined by broth microdilution assay for streptococci and lactobacilli reference, and for clinical strains. The effect of the essential oil on bacterial adhesion and biofilm formation/disruption was investigated. Negative (without treatment) and positive controls (chlorhexidine) were used. The effect of citral on preformed biofilm was also tested using the same methodology. Monospecies and microcosm biofilms were tested. ANOVA or Kruskal-Wallis tests were used (α=0.05). Cytotoxicity of the essential oil to human keratinocytes was performed by MTT assay. GC/MS demonstrated one major component (citral). The essential oil showed an inhibitory effect on all tested bacterial species, including S. mutans and L. acidophilus. Essential oil of C. citratus (10X MIC) reduced the number of viable cells of lactobacilli and streptococci biofilms (p < 0.05). The essential oil inhibited adhesion of caries-related polymicrobial biofilm to dental enamel (p < 0.01). Citral significantly reduced the number of viable cells of streptococci biofilm (p < 0.001). The essential oil showed low cytotoxicity to human keratinocytes. Based on these findings, this study can contribute to the development of new formulations for products like mouthwash, against dental biofilms.

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Section: Introductionmentioning

confidence: 99%

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Oliveira¹,

Borges²,

Brighenti

et al. 2017

Braz. oral res.

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“…Diversas publicações relataram atividade antimicrobiana das plantas aqui avaliadas 2,10,18,20 , entretanto, esse efeito é observado quando do uso na forma de óleos essenciais. Isso pode se dever ao fato de os principais fitoconstituintes serem voláteis, não sendo mantidas as concentrações quando as plantas são utilizadas em outras formulações 7 .…”

Section: Discussionunclassified

Atividade Antiaderente de Produtos Fitoterápicos sobre Streptococcus mutans

Silva

Valença²,

Costa³

et al. 2015

Rev. bras. ciênc. Saúde

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Introduction: Dental caries remains one of the most prevalent oral diseases, which encourages the development of new therapeutic alternatives aiming at its control. Objective: To evaluate the anti-adherent effects of phytotherapic agents against Streptococcus mutans (ATCC 25175). Material and Methods: The tinctures of Plectrantus amboinicus, Conyza bonariensis and Cymbopogon citratus, obtained in compounding pharmacy, were used in this study for determination of their Minimum Inhibitory Concentration of Adherence (MICA). Chlorhexidine (0.12%) and grain alcohol were used as the positive and negative controls, respectively. In order to determine the MICA, S. mutans was inoculated in Mueller Hinton broth supplemented with 10% sucrose in the presence of different concentrations of the products. Then the test tubes were placed in an incubator with a 30° tilt angle under microaerophilic atmosphere for 48 h. This procedure allowed for the development of an adhesive pellicle onto the glass. Subsequently, the tubes were washed with phosphate buffer solution and dyed with plaque disclosing solution (Replac). All tests were performed in duplicate and the data were analyzed using descriptive statistics. Results: The MICA of the tinctures that prevented S. mutans adherence to the glass tubes was 200 µg/mL. Conclusion: The tinctures proved to be effective in preventing S. mutans adherence to glass tubes.

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“…The essential oil extracted from the leaves presented microbicidal activity, microbicide on strains of Staphylococcus spp., Streptococcus mutans and Candida spp. (Almeida et al 2013); anxiolytic and anticonvulsive effects in mice (Blanco et al 2009;; anti-inflammatory action in rats (in vivo); antifungal activity in Candida albicans strains (Silva et al 2008), C. tropicalis and Aspergillus niger (in vitro) (Boukhatem et al 2014), and antimalarial activity in mice (in vivo) (Tchoumbougnang et al 2005). Polyphenols from C. citratus reduced the production of lipopolysaccharide-induced cytokines (LPS) in human and rat macrophages (in vitro) (Francisco et al 2013).…”

Section: Cymbopogon Citratus (Dc) Stapfmentioning

confidence: 99%

Medicinal plants for the "nerves": a review of ethnobotanical studies carried out in South Brazil

et al. 2019

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Among the medicinal purposes for which plants have been used is the treatment of "nerves". Th e objective of this study was to search for species of plants used in the state of Rio Grande do Sul (South Brazil) for the relief of symptoms related to central nervous system (CNS) disorders. Twenty-seven ethnobotanical studies were compiled, in which a total of 94 species were cited. Th e fi ve most cited species were Cymbopogon citratus (81.5 %), Melissa offi cinalis (77.7 %), Aloysia citriodora (66.6 %), Matricaria chamomilla (62.9 %) and Passifl ora edulis (51.8 %). Scientifi c studies have corroborated the popular use of these plants as sedatives, but most studies are preclinical and very few have been clinical (M. chamomilla and M. offi cinalis), and these were mainly exploratory or were performed against placebo. In addition to effi cacy data, there are also indications of toxicity for M. chamomilla and P. edulis. In conclusion, there is a great diversity of plant species used in the treatment of symptoms related to CNS disorders, and they are most frequently used as a sedative. Data indicate that M. offi cinalis possesses clinical effi cacy in the treatment of symptoms associated with anxiety without signs of toxicity.

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Antimicrobial activity of the essential oil of Cymbopogon citratus (DC) Stapf. on Staphylococcus spp., Streptococcus mutans and Candida spp.

Cited by 19 publications

References 35 publications

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Atividade Antiaderente de Produtos Fitoterápicos sobre Streptococcus mutans

Medicinal plants for the "nerves": a review of ethnobotanical studies carried out in South Brazil

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