Avaliação do perfil cromatográfico obtidos por CLAE-DAD e da atividade antioxidante das folhas de espécies Campomanesia sessiliflora (O. Berg) Mattos e Campomanesia xanthocarpa O. Berg

Abstract

Postal 351, RESUMO: O objetivo desta pesquisa visou a avaliação do perfil cromatográfico, a atividade antioxidante, e os teores de fenóis e flavonóides nos extratos orgânicos e infusos de folhas das espécies C. xanthocarpa e C. sessiliflora coletadas durante os períodos vegetativo e reprodutivo. As análises dos extratos e infusos foram realizadas empregando-se cromatografia líquida de alta eficiência com detector de arranjo de diodos (CLAE-DAD). Segundo a comparação dos perfis obtidos foi baixa a similaridade entre a composição química das duas espécies. O teor de fenóis (64,69-334,18 mg de ácido gálico mL -1 ) foi superior ao teor de flavonóides (4,02-35,60 mg de quercetina mL -1 ) em todas as análises. Os extratos orgânicos e infusos das folhas coletadas no período reprodutivo apresentaram teores de fenóis e de flavonóides superiores em ambas as espécies. Não houve uma correlação linear entre o teor de fenóis e flavonóides com a atividade antioxidante em todas as amostras. Palavras-chave:Campomanesia sessiliflora, Campomanesia xanthocarpa, CLAE-DAD, guavira, guabiroba ABSTRACT: Evaluation of the chromatographic profile and the antioxidant activity of the species Campomanesia sessiliflora (O. Berg) Mattos and Campomanesia xanthocarpa O. Berg. The aim of this study was to evaluate the chromatographic profile, the antioxidant activity, and the levels of phenols and flavonoids in organic extracts and infusions from leaves of the species C. xanthocarpa and C. sessiliflora collected during the vegetative and reproductive periods. Analyses of the extracts and infusions were performed by adopting high-performance liquid chromatography with diode array detector (HPLC-DAD). Based on the comparison of the obtained profiles, similarity between the chemical composition of the two species was low. Phenol levels (64.69-334.18 mg gallic acid mL -1 ) were superior to the level of flavonoids (4.02-35.60 mg quercetin mL -1 ) in all analyses. Organic extracts and infusions from leaves collected in the reproductive period had superior phenol and flavonoid levels in both species. There was not a linear correlation of phenol and flavonoid levels with antioxidant activity in all samples.

Photoprotective action, antioxidant activity, and toxicity of aqueous extracts of Campomanesia sessiliflora O. Berg

“…The ethanol extracts of the Amburana cearensis AC Smith bark and leaves of Croton sonderianus Muell and Sida galheirensis Ulbr contain IC50 of 54.88 µg•mL -1 , 51.40 µg•mL -1 and 57.41 µg•mL -1 respectively, with values similar to those analysed extracts [4]. The infusion of C. sessiliflora leaves presented antioxidant activity at different times of the year [40]. The extracts obtained by decoction of C. xanthocarpa leaves showed IC50 against the DPPH 38.47 µg•mL -1 [41].…”

Preparations from Campomanesia reitziana reduce the gastrointestinal motility and castor oil‐induced diarrhea in a non‐opioid and non‐dopaminergic pathway in mice and display antimicrobial activity in vitro

“…One of the consequences of diarrhea is the softening of stools or an increase in the number of watery stools together with abdominal cramps 1‐40 . Castor oil has been a useful experimental tool in the search for antidiarrheal preparations 41 given that it is hydrolyzed in the duodenum by pancreatic enzymes, leading to the release of ricinoleic acid that promotes alteration of electrolytes permeability on the intestinal mucosa increasing peristaltic activity 41,42 .…”

“…The aqueous extract by decoction (AECR) and dry residue from this aqueous extract (DR‐AECR), as well as the hydroalcoholic extract (HECR) from the leaves of C. reitziana and its ethyl acetate (EAF) and the dichloromethane (DCMF), were employed in this study aiming to identify a phytochemical extraction to concentrate compounds with antidiarrheal activity from this plant and mimicking popular use through decoction. First, given the presence of phenols and especially flavonoids in leaf preparations of this genus 29 the levels of these compounds were measured in C. reitziana preparations and the quantification of total phenols in DR‐EACR and HECR and fractions confirmed high levels of these compounds.…”

Antimicrobial Potential of Essential Oils from Cerrado Plants against Multidrug−Resistant Foodborne Microorganisms

“…The decoction of leaves of Chromolaena squalida , popularly known as cambará−roxa, mata−pasto, casadinha or erva−de−são−miguel [ 41 ], is used in folk medicine to treat cough and as an antipyretic [ 42 ]. The species Campomanesia sessiliflora , popularly known as guabiroba−verde, is frequently found in the Brazilian Cerrado [ 43 ] and produces edible fruits, consumed in natura by the local population. Matayba guianensis , known as brazeiro, camboatá, camboatã, camboatá−branco, olho−de−cotia, mataíba, batabaíba, cuvantã, jatuá−uba, jatuá−iba, pau−da−digestão, atou−aou, tou−aou or canela−de−negro [ 44 ], is considered a medicinal plant, and infusions and decocts of its root are used in the treatment of respiratory diseases and to alleviate back and leg pain [ 45 ].…”