Produção de mudas de carobinha (Jacaranda decurrens Cham.) em sistema de imersão temporária com biorreatores do tipo R.I.T.A.

Abstract

Produção de mudas de carobinha (Jacaranda decurrensCham Palavras-chave: multiplicação in vitro, otimização de protocolo, Jacaranda decurrens, planta medicinalABSTRACT: "Carobinha" seedling production in a temporary immersion system with R.I.T.A. bioreactors. The aim of this study was to identify the best explant density and the best cultivation system with the goal of developing a micropropagation protocol of low cost for "carobinha" (Jacaranda decurrens CHAM.). Experiments of in vitro multiplication were carried out using four flask types: R.I.T.A. (50 explants/flask), Erlenmeyer (50 explants/flask), mayonnaise pots (6 explants/flask) and cuvettes (1 explant/flask). The co-cultivation of explants, in both solid and liquid medium (R.I.T.A.), led explants to show higher sprouting and survival rates. The temporary immersion system provided better rates of development, sprouting, survival and height of explants. We concluded that bioreactors may be efficiently used for the micropropagation of "carobinha".