Efeito do composto "mais vida" na ativação de macrófagos de ratos diabéticos

Abstract

RESUMO:O objetivo deste trabalho foi avaliar a atividade funcional de macrófagos de ratos diabéticos, através da liberação do ânion superóxido, na presença do composto "mais vida". Os animais foram divididos em dois grupos, controle (N=20) e diabético (N=20). Avaliou-se a glicemia, massa corpórea e a liberação de superóxido pelos macrófagos de baço de ratos. O composto "mais vida" foi obtido através da mistura de extratos de sete plantas, sendo Orbignia martiana Rodr., Tabebuia avellanedae L.G., Arctium lappa L., Rosa centifolia L., Maytenus ilicifolia Mart., Vernonia condensata Baker e Thuja occidentalis L. Observou-se que glicemia foi maior no grupo diabético. A liberação espontânea do ânion superóxido pelos macrófagos foi menor no grupo diabético. O composto "mais vida", independente dos níveis glicêmicos, aumentou a liberação de superóxido dos macrófagos. Quando as células foram estimuladas pelos extratos vegetais isolados, também houve aumento na liberação do ânion superóxido pelos macrófagos em ambos os grupos. As maiores liberações de superóxido ocorreram quando os macrófagos foram estimulados pela Thuja occidentalis L., Rosa centifolia L., Tabebuia avellanedae L.G. e Maytenus ilicifolia Mart. Estes dados sugerem que a ativação de macrófagos pelo composto "mais vida" pode representar um mecanismo alternativo de defesa para infecções em indivíduos diabéticos.Palavras-chave: Fitoterápico, fagócitos, diabetes, aloxana, ânion superóxido ABSTRACT: Effects of "mais vida", a commercial natural mix, on the activation of macrophages from diabetic rats. This study investigated the effects of "mais vida", a commercial natural mix, on macrophages functional activity as evaluated by the superoxide release in diabetic rats. The animals were divided into two groups, control (N = 20) and diabetic (N = 20). This was achieved by determining blood glucose weight and the superoxide released by spleen macrophages. The "mais vida" mix was obtained by the combination of extracts from seven medicinal species, which were: Orbignia martiana Rodr., Tabebuia avellanedae L.G., Arctium lappa L., Rosa centifolia L., Maytenus ilicifolia Mart., Vernonia condensata Baker and Thuja occidentalis L. Blood glucose levels were significantly higher (p<0.05) in the diabetic group, as compared to blood glucose levels in the control group. Superoxide levels in macrophages isolated from normoglycemic rats were higher than those obtained from diabetic animals. The "mais vida" mix, independently of glycemic status, increased significantly the superoxide release in the macrophages. Each extract by itself also increased the superoxide release by phagocytes in the macrophages in both groups. The largest superoxide release occurred when the phagocytes were stimulated by Thuja occidentalis L., Rosa centifolia L., Tabebuia avellanedae L.G. and Maytenus ilicifolia Mart. In addition, the activation of macrophages by the "mais vida" mix may represent an additional protection mechanism for diabetic individuals against infections.

Superoxide Anion Release by Human Blood Phagocytes Can Increase the Microbicidal Activity Induced by a New Microemulsioned System Containing Cotton Oil

“…Oils in plants such as babassu and buriti have also been reported as important phagocyte modulators capable of increasing microbicidal activity [5] [6]. In addition, these natural products have shown functionalities in the immune system capable of treating several infections [55].…”

Desenvolvimento de nanocarreadores formados por micelas de pluronic F127 ou nanoemulsões para veiculação de interferon gamma e avaliação do potencial imunomodulador

“…The free radical release is an important mechanism of defense during infectious processes, mainly in intestinal infections [49,50,77]. It is known that cells are able to produce great amounts of superoxide radicals during an oxidative stress, but this process can be further stimulated by endogenous or exogenous compounds [85,86], such as melatonin [87,88] and active plants [82,89], and cytokines, such as IFN- [46,90] respectively. Here, our findings show that NE1IFN-formulation is able to stimulate the functional activity of human blood MN phagocytes by increasing superoxide release, and this increase reflects in phagocytosis and Ca 2+ intracellular release.…”