Variação de caracteres morfológicos e fisiológicos de populações naturais de Pfaffia glomerata (Spreng.) Pedersen e correlação com a produção de β-ecdisona

Kamada, Takeshi; Picoli, Edgard Augusto de Toledo; Vieira, R. F.; Barbosa, Luiz C. A.; Cruz, Cosme Damião; Otoni, Wagner Campos

doi:10.1590/s1516-05722009000300004

Cited by 12 publications

(2 citation statements)

References 14 publications

(1 reference statement)

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“…Moreover, according to the Zn responses, GD accession is the best candidate for field trials to test the value of herbaceous species to restore contaminated areas. Kamada et al (2009b) observed higher divergence among individuals of P. glomerata from different populations and lower divergence among those from the same population in relation to β-ecdysone tissue-specific content. Soil abiotic factors have not been studied in relation to root morphological parameters in P. glomerata.…”

Section: Resultsmentioning

confidence: 76%

Changes in root morphology and dry matter production in Pfaffia glomerata (Spreng.) Pedersen accessions in response to excessive zinc

Bernardy

Farias

Dorneles

et al. 2016

Rev. bras. plantas med.

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Zinc (Zn) participates of numerous metabolic processes in plants. However, it can become toxic to plants in excessive concentrations in the soil. Pfaffia glomerata is a Brazilian medicinal species that has stood out because of its numerous chemical and functional properties, mainly by the triterpene saponins and ecdysteroids accumulated in its roots. This study aimed to evaluate the effects of zinc excess on many root morphological parameters of Pfaffia glomerata. A 4 x 3 factorial design was employed in a completely randomized scheme with 3 replicates. The treatments consisted of four concentrations of Zn (2, 100, 200, and 300 µM) and three accessions of P. glomerata (BRA, GD, and JB) grown in a hydroponic system for 7 and 14 days. Differences in root morphology and dry mass production were observed among the three accessions in response to excessive Zn. Some growth parameters of GD accession increased with the addition of Zn, ranging from 36 to 79 µM. However, the GD and JB accessions presented reduction in dry mass production, root area, length, and volume with increasing Zn levels. The BRA accession, which had the lowest growth among accessions, presented chlorotic leaves. The shoot/root dry mass ratio and root diameter increased linearly for BRA and GD accessions at 7 days. Based on the evaluated parameters, we observed the following order of Zn excess tolerance in P. glomerata accessions: GD> JB> BRA.Keywords: growth, heavy metal, Brazilian ginseng, root length, root volume.RESUMO: Alterações na morfologia radicular e produção de matéria seca em acessos de Pfaffia glomerata (Spreng.) Pedersen em resposta ao excesso de zinco. O zinco (Zn) participa de numerosos processos metabólicos nas plantas. No entanto, em concentrações excessivas no solo pode tornar-se tóxico para os vegetais. Pfaffia glomerata é uma espécie medicinal brasileira que tem se destacado devido as suas inúmeras propriedades químicas e funcionais, devido principalmente às saponinas triterpênicas e ecdisteróides acumuladas em suas raízes. O objetivo do presente trabalho foi avaliar os efeitos do excesso de Zn sobre vários parâmetros morfológicos radiculares de Pfaffia glomerata. O delineamento experimental utilizado foi o inteiramente casualizado com três repetições, dispostos em um arranjo fatorial (4 x 3). Os tratamentos consistiram em quatro níveis de Zn (2, 100, 200 e 300µM) e três acessos (BRA, GD e JB) de P. glomerata cultivados em sistema hidropônico em casa de vegetação por 7 e 14 dias. Diferenças na morfologia radicular e na produção de material seca foram observadas entre os três acessos em resposta ao excesso de Zn. Alguns parâmetros de crescimento do acesso GD aumentaram sob a adição de Zn variando entre 36 e 79 µM. No entanto, os acessos GD e JB apresentaram redução na matéria seca, bem como na área de superfície, comprimento e volume radicular com o aumento dos níveis de Zn. O acesso BRA, que teve o menor crescimento entre os acessos, apresentou folhas cloróticas. A razão entre matéria seca da parte aérea e raízes e o ...

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Section: Resultsmentioning

confidence: 76%

Changes in root morphology and dry matter production in Pfaffia glomerata (Spreng.) Pedersen accessions in response to excessive zinc

Bernardy

Farias

Dorneles

et al. 2016

Rev. bras. plantas med.

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“…A 20 µl sample of each extract was injected and absorbance was monitored at 245 nm. The HPLC conditions utilized for β-ecdysone detection were optimized after preliminary assays using samples of P. glomerata (Magalhães, 2000;Figueiredo et al 2004;Flores et al 2009b;Kamada et al 2009). …”

Section: Methodsmentioning

confidence: 99%

Micropropagation and β-ecdysone content of the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa

Flores

Brondani

Cezarotto

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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The aim of this study was to investigate both a mass in vitro propagation system and the β-ecdysone content in roots and aerial parts of Pfaffia glomerata and Pfaffia tuberosa. Nodal segments of two genotypes (BRA and JB-UFSM) of P. glomerata, originated from aseptically grown plants, were cultivated on hormone-free Murashige and Skoog medium. For the proliferation of P. tuberosa shoots, nodal segments, originated from aseptically grown plants, were either cultivated on hormone-free Murashige and Skoog (MS) medium or were supplemented with 1.0 µM thidiazuron (TDZ); the elongation and rooting of these plants were carried out on MS medium without TDZ. Plantlets of both species were acclimatized and transferred to field conditions. The β-ecdysone content in the plants was determined by high performance liquid chromatography. The BRA genotype showed a greater in vitro proliferation rate and β-ecdysone content than that of the JB-UFSM genotype. The culture of nodal segments of P. tuberosa on medium with 1.0 µM TDZ with subsequent subcultivation of shoots on hormone-free medium was shown to be a suitable method for micropropagation due to the high multiplication rate and good plant development. Both species showed good adaptation to ex vitro conditions. The β-ecdysone content in micropropagated P. tuberosa was similar to that found in field-grown plants. For both species, the aerial parts accumulated higher β-ecdysone content than roots. These results reveal that micropropagation is a successful, alternative method for rapid plant multiplication of both species of Brazilian ginseng. Furthermore, this study demonstrates that these two species have a potential for cultivation that is associated with high β-ecdysone production.

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De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production

Batista

Koehler

Romanel³

et al. 2018

Protoplasma

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Variação de caracteres morfológicos e fisiológicos de populações naturais de Pfaffia glomerata (Spreng.) Pedersen e correlação com a produção de β-ecdisona

Cited by 12 publications

References 14 publications

Changes in root morphology and dry matter production in Pfaffia glomerata (Spreng.) Pedersen accessions in response to excessive zinc

Changes in root morphology and dry matter production in Pfaffia glomerata (Spreng.) Pedersen accessions in response to excessive zinc

Micropropagation and β-ecdysone content of the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa

De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production

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