Desnutrição neonatal e produção de IFN-γ IL-12 e IL-10 por macrófagos/linfócitos: estudo da infecção celular, in vitro, por Staphylococcus aureus meticilina sensível e meticilina resistente

Costa, Thacianna Barreto da; Morais, Natália Gomes de; Almeida, Thays Miranda de; Severo, Maiara S.; Castro, Célia Maria Machado Barbosa de

doi:10.1590/s1415-52732012000500006

Cited by 6 publications

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“…Numerous clinical studies on morbidity and mortality rates have indicated that MRSA is more virulent than MSSA. However, laboratory studies that assessed the presence and magnitude of pathogenic mechanisms and virulence factors in MSSA and MRSA strains obtained conflicting results 5,10 .…”

Section: Introductionmentioning

confidence: 99%

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Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin

et al. 2014

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OBJECTIVE: To assess microbicide function and macrophage viability after in vitro cellular infection by methicillin-sensitive/resistant Staphylococcus aureus in nourished rats and rats subjected to neonatal malnutrition. METHODS: Male Wistar rats (n=40) were divided in two groups: Nourished (rats suckled by dams consuming a 17% casein diet) and Malnourished (rats suckled by dams consuming an 8% casein diet). Macrophages were recovered after tracheotomy, by bronchoalveolar lavage. After mononuclear cell isolation, four systems were established: negative control composed exclusively of phagocytes; positive control composed of macrophages plus lipopolysaccharide; and two testing systems, macrophages plus methicillin-sensitive Staphylococcus aureus and macrophages plus methicillin-resistant Staphylococcus aureus. The plates were incubated in a humid atmosphere at 37 degrees Celsius containing 5% CO2 for 24 hours. After this period tests the microbicidal response, cytokine production, and cell viability were analyzed. The statistical analysis consisted of analysis of variance (p<0.05). RESULTS: Malnutrition reduced weight gain, rate of phagocytosis, production of superoxide anion and nitric oxide, and macrophage viability. Production of nitrite and interleukin 18, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus were lower. CONCLUSION: The neonatal malnutrition model compromised phagocyte function and reduced microbicidal response and cell viability. Interaction between malnutrition and the methicillin-resistant strain decreased the production of inflammatory mediators by effector cells of the immune response, which may compromise the immune system's defense ability.

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Section: Introductionmentioning

confidence: 99%

“…This result is similar to that found by Melo et al 4 , but they used a regional basic diet low in all constituents. Costa et al 5 used an 8% casein diet to induce malnutrition and also found that weight gain decreased after the fourth day of life.…”

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confidence: 99%