Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)

Almeida, Pedro Marcos de; Araújo, Silvany de Sousa; Marin-Morales, Maria Aparecida; Benko‐Iseppon, Ana Maria; Brasileiro-Vidal, Ana Christina

doi:10.1590/s1415-475738120140162

Cited by 15 publications

(8 citation statements)

References 45 publications

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“…In the in vitro cytotoxicity assay against HEK-293 cells, at concentrations of 62.5; 125; 250 and 500 μg/mL, from the crude extract of the leaves of J. gossypiifolia obtained by decoction, no cytotoxic effect, was evidenced, therefore, being considered 100% cell viability. 18 This data contradict those obtained in this research, since the cytotoxicity test of the crude extracts of the stem to the macrophages presented slightly cytotoxic results, the leaves were moderately cytotoxic; and the branches presented cell viability of less than 50%.…”

Section:  In Vitro Healing Potential Using Scratch Assaycontrasting

confidence: 90%

Cytotoxic, antimicrobial and healing activity of the Jatropha gossypiifolia L extract.

Silva¹,

Lopes²,

Silva³

et al. 2018

Rev enferm UFPE on line.

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RESUMOObjetivo: investigar o potencial citotóxico, antimicrobiano e cicatrizante de extratos das folhas, galhos e caule da J. gossypiifolia L. Método: estudo quantitativo, experimental. Os extratos foram obtidos por maceração em etanol, concentrados em evaporador rotatório e secos em dessecador a vácuo. Na análise, realizaram-se testes de prospecção fitoquímica; citotoxidade; microdiluição em caldo e Scratch assay. Entre os metabólitos detectados, estiveram: taninos, esteroides, flavonoides, flavonas, xantonas. Resultados: o extrato do caule apresentou viabilidade celular acima de 80%. As folhas foram moderadamente citotóxicas e os galhos apresentaram ausência de viabilidade celular. Os extratos inibiram o crescimento de S. aureus, S. epidermidis e P. aeruginosa em diferentes concentrações. O Scratch assay evidenciou que a fração metanólica das folhas propiciou a migração celular em 45% a mais do que o controle. Conclusão: estudos com esta espécie vegetal devem ser continuados para isolamento do princípio ativo, visando à produção de um fitoterápico cicatrizante de feridas. Descritores: Cicatrização; Enfermagem; Citoxidade; Plantas Medicinais.ABSTRACTObjective: to investigate the cytotoxic, antimicrobial and cicatrizant potential of extracts of leaves, branches and stem of J. gossypiifolia L. Method: quantitative, experimental study. The extracts were obtained by maceration in ethanol, concentrated in a rotary evaporator and dried in a vacuum desiccator. In the analysis, phytochemical prospecting; cytotoxicity; microdilution in broth and Scratch assay tests were performed. Among the detected metabolites, were: tannins, steroids, flavonoids, flavones, xanthones. Results: the stem extract presented cell viability above 80%. The leaves were moderately cytotoxic and the branches showed no cell viability. The extracts inhibited the growth of S. aureus, S. epidermidis and P. aeruginosa at different concentrations. Scratch assay showed that the methanolic fraction of the leaves allowed the cellular migration in 45% more than the control. Conclusion: studies with this plant species should be continued for isolation of the active principle, aiming at the production of a wound healing phytotherapic. Descriptors: Healing; Nursing; Citotoxicity; Medicinal Plants.RESUMENObjetivo: investigar el potencial citotóxico, antimicrobiano y cicatrizante de los extractos de las hojas, ramas y el tallo de J. gossypiifolia L. Método: estudio cuantitativo, experimental. Los extractos fueron obtenidos por maceración en etanol, concentrados en evaporador rotatorio y seco en desecador al vacuo. En el análisis, se realizaron pruebas de prospección fitoquímica; citotoxicidad; microdilución en caldo y Scratch asay. Entre los metabolitos detectados, estuvieron: taninos, esteroides, flavonoides, flavonas, xantonas. Resultados: el extracto del tallo presentó viabilidad celular por encima del 80%. Las hojas fueron moderadamente citotóxicas y las ramas presentaron ausencia de viabilidad celular. Los extractos inhibieron el crecimiento de S. aureus, S. epidermidis y P. aeruginosa en diferentes concentraciones. El Scratch assay evidenció que la fracción metanólica de las hojas propició la migración celular en un 45% más que el control. Conclusión: estudios con esta especie vegetal deben ser continuados para aislamiento del principio activo, visando la producción de un fitoterápico cicatrizante de heridas. Descriptores: La Curación; Enfermería; Citotoxicidad; Plantas Medicinales.

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Section:  In Vitro Healing Potential Using Scratch Assaycontrasting

confidence: 90%

Cytotoxic, antimicrobial and healing activity of the Jatropha gossypiifolia L extract.

Silva¹,

Lopes²,

Silva³

et al. 2018

Rev enferm UFPE on line.

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“…According to this broad analysis, it is likely that alkaloids were the phytochemical compounds responsible for wound healing activity because they were not detected in the chloroform fraction. Although the composition of latex is similar among Jatropha species, some phytochemicals are present at lower concentrations or completely absent depending on the specific Jatropha species [ 10 , 15 , 24 , 25 ]. The differences in latex phytochemical composition detected among different published papers may suggest that composition is dependent on the species of Jatropha species and the sensitivity of the analytical techniques used.…”

Section: Discussionmentioning

confidence: 99%

The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model

et al. 2020

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Background and Aim: Jatropha gaumeri Greenm. is commonly used to treat mouth blisters and skin rashes, its latex has analgesic and anti-inflammatory activity on buccal ulcer. This study aimed to demonstrate the wound healing activity of a cream formulation of Jatropha gaumeri Greenm. latex in a murine model, provide a histological assessment of its scarring effects, and identify the family of phytochemicals involved in these effects. Materials and Methods: Latex was obtained from the cut stalk leaves and young stems of J. gaumeri and stored in sterile tubes with protection from light. Chloroform, ethyl acetate, and aqueous fractions of the latex were obtained. Fifty male Balb/c mice aged 10-12 weeks were divided into 10 groups of five mice: Group 1 corresponded to healthy mice with wounds; Group 2 corresponded to mice with wounds and treated with A-Derma®; and from Group 3 to group 10 corresponded to mice treated with a different latex fraction. A circular skin wound of about 1 cm was made on the paravertebral region of each mouse under anesthetized and aseptic conditions. The wounds were topically treated every 24 h with the respective extracts for 22 days, after which skin tissue specimens were obtained and stained with hematoxylin-eosin and Masson's trichrome. The efficiency of healing was measured by quantifying the tensile strength of the scars. The phytochemicals in the latex were elucidated using thin chromatography. Results: The aqueous latex fraction produced the best wound healing activity and was superior to the positive control. Reepithelialization at the histological level resulted in tissue that resembled healthy skin in terms of the appearance of collagen, the regeneration of hair follicles, and cellularity of the dermis, which showed organized epithelialization. A wound healing efficacy of 97% was observed, and it seems that alkaloids were the phytochemicals mostly likely responsible for these effects. Conclusion: J. gaumeri latex exhibited wound healing activity, possibly mediated by phytochemicals such as alkaloids in the aqueous fraction.

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“…Similarly to what was observed to J. curcas latex, J. gossypiifolia latex showed a significant decrease in root growth and in the mitotic index for the tested concentrations (0.1%, 0.2%, 0.5%, 1% and 2%). The 0.1%, 0.2%, 0.5% concentrations induced significant chromosome adherences, C-metaphases and/or chromosome bridges, suggesting genotoxic effects (ALMEIDA et al, 2015).…”

Section: Discussionmentioning

confidence: 94%

Toxicity of Jatropha curcas L. latex in Allium cepa test

Ciappina¹,

Ferreira²,

Pereira³

et al. 2017

Biosci. J.

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ABSTRACT:The latex obtained from Jatropha curcas (physic nut) is used in traditional medicine to treat a variety of disturbs, including burns, hemorrhoids, ringworm and ulcers. Phytochemical analyses have shown that J. curcas latex contains natural compounds with therapeutic potential. In this study, the toxicity, cytotoxicity and genotoxicity effects of J. curcas latex on the root cells of Allium cepa were examined. Onion seeds and bulbs were exposed to seven different concentrations of latex and then the roots were submitted to macro and microscopic analyses. Water and sodium azide were used as negative and positive controls, respectively. The analysis of root growth showed that J. curcas crude latex or 50% diluted is highly toxic. Cytogenetic results showed that the mitotic index of the onion roots submitted to latex treatment decreased significantly compared to the negative control, which suggests that the latex is cytotoxic. High incidence of chromosome aberrations in the cells treated with J. curcas latex was observed too, indicating that the latex also presents genotoxic effect. The analyses presented in this report suggest the toxic, cytotoxic and genotoxic effects of J. curcas latex. Then, the indiscriminate use of J. curcas latex in folk medicine could bring risk to human health.

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Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)

Cited by 15 publications

References 45 publications

Cytotoxic, antimicrobial and healing activity of the Jatropha gossypiifolia L extract.

Cytotoxic, antimicrobial and healing activity of the Jatropha gossypiifolia L extract.

The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model

Toxicity of Jatropha curcas L. latex in Allium cepa test

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