An effective homologous cloning method for isolating novel miR172s from Phalaenopsis hybrida

Han, Ying; Yan, Qin Hua; Ming, Feng

doi:10.1590/s1415-47572014005000004

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…It is known that the expression of miRNAs in other plants depends on the type of tissue analyzed. For instance, in rice, miR172c is expressed in seedlings but not in grains under development, and in Phalaenopsis hybrida, the PhmiR172-1 and PhmiR172-2 variants were detected in stems, pedicels, roots, and meristems, but not in leaves, petals, and sepals (Sunkar et al 2008;Zhu et al 2008;Han et al 2014). …”

Section: Expression Analysis Of Mir172 and Mir156mentioning

confidence: 99%

Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L.

et al. 2017

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Jatropha curcas is a plant that accumulates high-quality oil in its seeds, which is capable of being transformed into liquid biofuel. However, several aspects of its fl owering are still unknown, which is a key step in the production of fruits, seeds, and oil. Flowering is a complex process that is regulated by various factors at the molecular level, including microRNAs (miRNAs). Th ere is evidence suggesting that the miRNAs of the miR156 and miR172 families play a key role in the fl owering transition of plants. For this reason, the dynamics of miR156 and miR172 were studied during a production cycle of J. curcas. Our results reveal that J. curcas has a mechanism for the expression of these miRNAs that diff ers from that reported for the vast majority of angiosperms, since the expression of both families of miRNAs was positively correlated with the phenological state of J. curcas. We discuss the implications of these fi ndings and how the regulatory mechanisms of miR156 and miR172 diff er from what has been reported thus far.

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Section: Expression Analysis Of Mir172 and Mir156mentioning

confidence: 99%

Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L.

et al. 2017

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“…In this study, a CYC-B gene orthologue was successfully identified from the loquat using a homology-based cloning method, a simple, fast, and efficient method that uses highly conserved sequences to design degenerate primers to clone target genes. This has been used for carrots [34], Phalaenopsis Hybrida [35], and tobacco [36], etc.…”

Section: Discussionmentioning

confidence: 99%

Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

et al. 2019

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Carotenoids are the principal pigments in the loquat. Although the metabolic pathway of plant carotenoids has been extensively investigated, few studies have been explored the regulatory mechanisms of loquat carotenoids because knowledge of the loquat genome is incomplete. The chromoplast-specific lycopene β-cyclase gene (CYC-B) could catalyze cyclization of lycopene to β-carotene. In this study, the differential accumulation patterns of loquat with different colors were analyzed and virus-induced gene silencing (VIGS) was utilized in order to verify CYC-B gene function. Using a cloning strategy of homologous genes, a CYC-B gene orthologue was successfully identified from the loquat. At a later stage of maturation, CYC-B gene expression and carotenoids concentrations in the 'Dawuxing' variety were higher than in 'Chuannong 1-5-9 , possibly leading to the difference in pulp coloration of loquat. Interference of CYC-B gene expression in the loquat demonstrated clear visual changes. The green color in negative control fruits became yellow, while TRV2-CYC-B silenced fruits remained green. CYC-B gene expression and total carotenoid content in the pulp decreased by 32.5% and 44.1%, respectively. Furthermore, multiple key genes in the carotenoid metabolic pathway synergistically responded to downregulation of CYC-B gene expression. In summary, we provide direct evidences that CYC-B gene is involved in carotenoid accumulation and coloration in the loquat.They participate in the assembly of photosystems and perform a significant role in light capture, whereby they absorb light in a range of the blue spectrum broader than that achieved in chlorophyll. The excited carotenoids undergo excitation energy transfer to chlorophyll [8,9]. Carotenoids protect the photosynthetic apparatus from photooxidative damage caused by excess light energy through the quenching of both singlet and triplet state chlorophylls [9,10]. Carotenoids are conducive to produce scents and flavors that attract insects and animals for pollination and seed dispersal [11]. They are also significant precursors for the biosynthesis of metabolites, such as vitamin A, strigolactone, and abscisic acid [12][13][14][15][16]. Furthermore, carotenoids are thought to protect the human body from oxidative stress by removing free radicals, which can prevent cancer, cardiovascular diseases, and other chronic diseases [17][18][19].Sites for the biosynthesis and accumulation of plant carotenoids are plastids, containing chloroplasts and chromoplasts [20]. The plant carotenoid biosynthesis and cleavage pathway is shown in Supplementary Figure S1. Geranylgeranyl diphosphate (GGPP), a precursor of carotenoid metabolism, is catalyzed to produce phytoene by phytoene synthase (PSY). Phytoene is the first carotenoid molecule and undergoes desaturation and isomerization to form lycopene. This process is catalyzed by desaturases (phytoene desaturase, PDS; ζ-carotene desaturase, ZDS) and isomerases (carotene isomerase, CRTISO; 15-cis-ζ-carotene isomerase, ZISO). Cyclization of lycop...

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An effective homologous cloning method for isolating novel miR172s from Phalaenopsis hybrida

Cited by 2 publications

References 30 publications

Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L.

Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L.

Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

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