Phylogeny and molecular dating of the cerato-platanin-encoding genes

Yu, Huanyun; Li, Lin

doi:10.1590/s1415-47572014005000003

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…The MP‐based phylogenetic analysis, beside grouping the endophytes within expected taxonomic affinities, also revealed an interesting phylogenetic relationship between the members of Eurotiales (Ascomycetes) and Agaricales and Polyporales (Basidiomycetes). The phylogenetic grouping of species within Eurotiales with species of Agaricales, though surprising, has also been reported by Yu and Li [49] based on cerato‐platanin encoding genes.…”

Section: Discussionsupporting

confidence: 65%

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

Hajong

Kapoor

2020

J Basic Microbiol

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Endophytic fungi are known to play an important role in driving the evolution of plants by conferring adaptational advantages to their host through the production of secondary metabolites and phytohormones. In this study, we evaluated the diversity and phylogenetic relationship of endophytic fungal communities from four Dendrobium species viz., Dendrobium chrysanthum, Dendrobium heterocarpum, Dendrobium hookerianum, and Dendrobium longicornu of Meghalaya, India. A total of 51 culturable endophytic fungi were isolated from the four selected orchid species. The isolates were identified based on nuclear large subunit sequences into 33 species. Approximately 91% of the isolates showed affinity to Ascomycetes, while 9% of the isolates showed BLAST search similarity to Basidiomycetes. The most common genera were Trichoderma and Xylaria. The most prevalent genera were Fusarium, which was detected in all the four Dendrobium species followed by Diaporthe, which was present in three Dendrobium species viz., D. chrysanthum, D. hookerianum, and D. heterocarpum. The Shannon index value of endophytic fungal communities was the highest in D. chrysanthum (2.66), while D. longicornu (1) had the highest Evenness index. The present study revealed that endophytic fungi in these orchids are an amalgam of pathogenic and beneficial fungi, which have, at the least, switched their lifestyle to asymptomatic endophyte in their host. To our knowledge, this is the first such report on the diversity of endophytic fungi in the four selected Dendrobium species from Meghalaya, India.

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Section: Discussionsupporting

confidence: 65%

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

Hajong

Kapoor

2020

J Basic Microbiol

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“…Some CPs were found to act as allergens while others are elicitors and phytotoxins [ 56 ]. Baccelli [ 57 ] proposed that the fungal specific CP family proteins exhibit expansin-like activity during fungus-plant interactions and may play multiple biological roles associated to the fungal growth and development, parasitism, cell wall morphogenesis, immune response, and chemotaxis [ 58 ]. Phylogenetic analysis of close homologs of CP family members along with previously reported CPs shows that they can be grouped in two major clades corresponding to their origins from basidiomycetes or ascomycetes ( Fig 7 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis Revealed Highly Expressed Genes Encoding Secondary Metabolite Pathways and Small Cysteine-Rich Proteins in the Sclerotium of Lignosus rhinocerotis

et al. 2015

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Lignosus rhinocerotis (Cooke) Ryvarden (tiger milk mushroom) has long been known for its nutritional and medicinal benefits among the local communities in Southeast Asia. However, the molecular and genetic basis of its medicinal and nutraceutical properties at transcriptional level have not been investigated. In this study, the transcriptome of L. rhinocerotis sclerotium, the part with medicinal value, was analyzed using high-throughput Illumina HiSeqTM platform with good sequencing quality and alignment results. A total of 3,673, 117, and 59,649 events of alternative splicing, novel transcripts, and SNP variation were found to enrich its current genome database. A large number of transcripts were expressed and involved in the processing of gene information and carbohydrate metabolism. A few highly expressed genes encoding the cysteine-rich cerato-platanin, hydrophobins, and sugar-binding lectins were identified and their possible roles in L. rhinocerotis were discussed. Genes encoding enzymes involved in the biosynthesis of glucans, six gene clusters encoding four terpene synthases and one each of non-ribosomal peptide synthetase and polyketide synthase, and 109 transcribed cytochrome P450 sequences were also identified in the transcriptome. The data from this study forms a valuable foundation for future research in the exploitation of this mushroom in pharmacological and industrial applications.

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“…Cerato-platanins (CPs) are small fungal proteins widely found in basidiomycetes and ascomycetes (Yu and Li 2014). Their name derives from the plant pathogenic fungus Ceratocystis platani (Pazzagli et al 1999).…”

Section: Swollenins Cerato-platanins and Other Expansin-like Proteinsmentioning

confidence: 99%

Bacterial expansins and related proteins from the world of microbes

Georgelis

Nikolaidis

Cosgrove

2015

Appl Microbiol Biotechnol

106

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The discovery of microbial expansins emerged from studies of the mechanism of plant cell growth and the molecular basis of plant cell wall extensibility. Expansins are wall-loosening proteins that are universal in the plant kingdom and are also found in a small set of phylogenetically diverse bacteria, fungi, and other organisms, most of which colonize plant surfaces. They loosen plant cell walls without detectable lytic activity. Bacterial expansins have attracted considerable attention recently for their potential use in cellulosic biomass conversion for biofuel production, as a means to disaggregate cellulosic structures by non-lytic means (‘amorphogenesis’). Evolutionary analysis indicates that microbial expansins originated by multiple horizontal gene transfers from plants. Crystallographic analysis of BsEXLX1, the expansin from Bacillus subtilis, shows that microbial expansins consist of two tightly-packed domains: the N-terminal domain D1 has a double-ψ β-barrel fold similar to glycosyl hydrolase family-45 enzymes, but lacks catalytic residues usually required for hydrolysis; the C-terminal domain D2 has a unique β-sandwich fold with three co-linear aromatic residues that bind β-1,4-glucans by hydrophobic interactions. Genetic deletion of expansin in Bacillus and Clavibacter cripples their ability to colonize plant tissues. We assess reports that expansin addition enhances cellulose breakdown by cellulase and compare expansins with distantly related proteins named swollenin, cerato-platanin and loosenin. We end in a speculative vein about the biological roles of microbial expansins and their potential applications. Advances in this field will be aided by a deeper understanding of how these proteins modify cellulosic structures.

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Phylogeny and molecular dating of the cerato-platanin-encoding genes

Cited by 10 publications

References 15 publications

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

Transcriptome Analysis Revealed Highly Expressed Genes Encoding Secondary Metabolite Pathways and Small Cysteine-Rich Proteins in the Sclerotium of Lignosus rhinocerotis

Bacterial expansins and related proteins from the world of microbes

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