bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells

Song, Xinghui; Li, Yanwei; Xiao, Chen; Yin, Guoli; Huang, Qiong; Chen, Yingying; Xu, Guangyu; Wang, Linlin

doi:10.1590/s1415-47572014000100019

Cited by 14 publications

(5 citation statements)

References 30 publications

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“…EGF and bFGF have been shown to improve the adipocyte differentiation capacity of MSCs [43, 45, 46], and bFGF addition during the expansion of hMSCs decreases the expression of the cell marker CD90 [35, 44]. We previously showed that MSCs maintained in serum-free and bFGF/EGF-supplemented medium have low CD90 expression levels [31].…”

Section: Discussionmentioning

confidence: 99%

The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

et al. 2019

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AbstractsBackgroundSpinal cord injury (SCI) is a complex and severe neurological condition. Mesenchymal stem cells (MSCs) and their secreted factors show promising potential for regenerative medicine. Many studies have investigated MSC expansion efficacy of all kinds of culture medium formulations, such as growth factor-supplemented or xeno-free medium. However, very few studies have focused on the potential of human MSC (hMSC) culture medium formulations for injured spinal cord repair. In this study, we investigated the effect of hMSC-conditioned medium supplemented with bFGF, EGF, and patient plasma, namely, neural regeneration laboratory medium (NRLM), on SCI in vitro and in vivo.MethodsCommercial and patient bone marrow hMSCs were obtained for cultivation in standard medium and NRLM separately. Several characteristics, including CD marker expression, differentiation, and growth curves, were compared between MSCs cultured in standard medium and NRLM. Additionally, we investigated the effect of the conditioned medium (referred to as NRLM-CM) on neural repair, including inflammation inhibition, neurite regeneration, and spinal cord injury (SCI), and used a coculture system to detect the neural repair function of NRLM-MSCs.ResultsCompared to standard culture medium, NRLM-CM had superior in inflammation reduction and neurite regeneration effects in vitro and improved functional restoration in SCI rats in vivo. In comparison with standard culture medium MSCs, NRLM-MSCs proliferated faster regardless of the age of the donor. NRLM-MSCs also showed increased adipose differentiative potential and reduced CD90 expression. Both types of hMSC CM effectively enhanced injured neurite outgrowth and protected against H2O2 toxicity in spinal cord neuron cultures. Cytokine arrays performed in hMSC-CM further revealed the presence of at least 120 proteins. Among these proteins, 6 demonstrated significantly increased expression in NRLM-CM: adiponectin (Acrp30), angiogenin (ANG), HGF, NAP-2, uPAR, and IGFBP2.ConclusionsThe NRLM culture system provides rapid expansion effects and functional hMSCs. The superiority of the derived conditioned medium on neural repair shows potential for future clinical applications.

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Section: Discussionmentioning

confidence: 99%

The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

et al. 2019

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“…Further, carrageenan inhibits insulin signalling (Bhattacharyya et al, 2015;Bhattacharyya et al, 2012), which is a potent adipogenic hormone (Klemm et al, 2001). Also, carrageenan may have interacted with FGF-2, which stimulates adipogenic differentiation of mesenchymal stem cells (Kakudo et al, 2007;Kim et al, 2015;Song et al, 2014); after all, a chitosan/ fucoidan hydrogel has been shown to enhance the half-life of FGF-2 (Nakamura et al, 2008) and numerous studies have reported the interaction of www.ecmjournal.org When BMSCs were trypsinised at the end of the pre-condition phase and seeded in new wells for the osteogenic differentiation phase, at day 12, (a) gross visual inspection and (b) DNA quantification revealed that the number of cells that were grown in the presence of MMC in the pre-condition phase (+/− and +/+) was significantly lower then the number of cells that were grown in the absence of MMC in the pre-condition phase (−/− and −/+). (c) The highest calcium content was detected with cells that were grown in the presence of MMC in both pre-condition and differentiation phases (+/+).…”

Section: Discussionmentioning

confidence: 99%

Carrageenan enhances chondrogenesis and osteogenesis in human bone marrow stem cell culture

Graceffa¹,

Zeugolis

2019

eCM

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The extracellular matrix is a dynamic and active component of the mesenchymal stem cell niche, which controls their differentiation and self-renewal. Traditional in vitro culture systems are not able to mimic matrix-cell interactions due to the small amount of extracellular matrix present. Macromolecular crowding, a biophysical phenomenon based on the excluded-volume effect, dramatically accelerates and increases tissue-specific extracellular matrix deposition during in vitro culture. Herein, the influence of macromolecular crowding in precondition and tri-lineage differentiation of human bone marrow mesenchymal stem cells was investigated. Carrageenan, a sulphated polysaccharide, enhanced chondrogenesis, as evidenced by increased collagen type II and chondroitin sulphate deposition and unaffected Sox-9 expression. Osteogenesis was also enhanced when carrageenan was used only in the differentiation phase, as evidenced by increased mineralisation, collagen type I deposition and osteopontin expression. Adipogenesis was not enhanced in the presence of carrageenan, suggesting that the chemistry of the crowder may affect stem-cell-lineage commitment. In conclusion, carrageenan, a sulphated polysaccharide, enhanced extracellular matrix deposition and promoted chondrogenesis and osteogenesis but not adipogenesis in human bone marrow mesenchymal stem cell cultures.

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“…At the pre-implantation stage, murine embryos, human germ cells, and teratocarcinoma stem cells express certain molecular receptors known as Stage Specific Embryonic Antigens (SSEA) on their membrane surface [ 36 ]. SSEA-1 and Nanog occur in embryonic stem cells; both have an important role in maintaining the capacity for self-renewal [ 37 ]. Thus these markers have been used to characterize mouse and human embryonic stem cells [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of adipose-derived stem cells of anatomical region from mice

et al. 2014

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BackgroundStem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential. Several studies have provided evidence that adipose tissue represents an alternative source of stem cells, with the main benefit of adipose-derived stem cells being that they can be easily harvested from patients by a simple minimally invasive method and can be easily cultured. The aim of this study was to establish a culture protocol for obtaining and characterizing adipose-derived stem cells (ADSCs) from C57BL/6 J mice.ResultsThe results showed that the yield, viability, and cell morphology obtained differ according to the age of isolated anatomic regions of the adipose tissue from ovarian and epididymis. The results of determination of cyclin D1 showed uniformity in the expression between different populations of ADSCs. A significant increase in the expression of caspase-3 active, was also observed in large cell populations from mice after 120 days. ADSCs were positive for mesenchymal markers CD90 and CD105, Nanog, SSEA-1, CD106, and VEGFR-1, and negative for hematopoietic markers CD34 and CD45. A large number of cells in S + G2/M phases was also observed for both sexes, demonstrating high proliferative capacity of ADSCs.ConclusionsWe observed that the adipose tissue of C57BL/6 J mice, isolated from the studied anatomic regions, is a promising source for obtaining pluripotent mesenchymal stem cells with high viability and proliferative response.

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bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells

Cited by 14 publications

References 30 publications

The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

Carrageenan enhances chondrogenesis and osteogenesis in human bone marrow stem cell culture

Characterization of adipose-derived stem cells of anatomical region from mice

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