Cloning and characterization of endo-β-1,4-glucanase genes in the common wheat line three pistils

Yang, Zaijun; Peng, Zhengsong; Wei, Shuhong; Yu, Yan

doi:10.1590/s1415-47572013000300015

Cited by 6 publications

(3 citation statements)

References 33 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The coexpression cluster contained 150 genes, which were involved in different processes (alcohol metabolism, cellulose catabolism, cell division, fungal growth, lipolysis, proteolysis, and transporters) (Figure 2A). We observed that cellobiohydrolases (50), cellulose-binding domains (51), glucanases (52), glycoside hydrolases (53), xylanases (54), and sugar transporters are the main genes present in the cluster, supporting the function of this cluster in cellobiose degradation and uptake of sugars. The cluster page also contains information about the enriched GO terms, whether similar clusters can be found in other fungi, presence of InterPro domains, enriched GO terms, and presence of gene families.…”

Section: Example 2: Gene Ontology Search Reveals Clusters Important Fsupporting

confidence: 54%

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

Lim

Koh

Moo

et al. 2020

Preprint

View full text Add to dashboard Cite

ABSTRACTThe fungi kingdom is composed of eukaryotic heterotrophs, which are responsible for balancing the ecosystem and play a major role as decomposers. They also produce a vast diversity of secondary metabolites, which have antibiotic or pharmacological properties. However, our lack of knowledge of gene function in fungi precludes us from tailoring them to our needs and tapping into their metabolic diversity. To remedy this, we gathered genomic and gene expression data of 19 most widely-researched fungi to build a database, fungi.guru, which contains tools for cross-species identification of conserved pathways, functional gene modules, and gene families. We exemplify how our database can elucidate the molecular function, biological process and cellular component of genes involved in various biological processes, by identifying a secondary metabolite pathway producing gliotoxin in Aspergillus fumigatus, the catabolic pathway of cellulose in Coprinopsis cinerea and the conserved DNA replication pathway in Fusarium graminearum and Pyricularia oryzae. The database is available at www.fungi.guru.

show abstract

Section: Example 2: Gene Ontology Search Reveals Clusters Important Fsupporting

confidence: 54%

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

Lim

Koh

Moo

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Compared with studies on the functions of single or few genes during flower development [ 13 , 14 ], the underlying genetic determinants that control flower development have received relatively little attention in wheat. Moreover, the genes and their corresponding expression patterns related to pistil and stamen development have yet to be reported.…”

Section: Introductionmentioning

confidence: 99%

Pistillody mutant reveals key insights into stamen and pistil development in wheat (Triticum aestivum L.)

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundThe pistillody mutant wheat (Triticum aestivum L.) plant HTS-1 exhibits homeotic transformation of stamens into pistils or pistil-like structures. Unlike common wheat varieties, HTS-1 produces three to six pistils per floret, potentially increasing the yield. Thus, HTS-1 is highly valuable in the study of floral development in wheat. In this study, we conducted RNA sequencing of the transcriptomes of the pistillody stamen (PS) and the pistil (P) from HTS-1 plants, and the stamen (S) from the non-pistillody control variety Chinese Spring TP to gain insights into pistil and stamen development in wheat.ResultsApproximately 40 Gb of processed reads were obtained from PS, P, and S. De novo assembly yielded 121,210 putative unigenes, with a mean length of 695 bp. Among these high-quality unigenes, 59,199 (48.84%) had at least one significant match with an existing gene model. A total of 23, 263, and 553 differentially expressed genes were identified in PS vs. P, PS vs. S, and P vs. S, respectively, with differences in expression greater than five-fold. Among the differentially expressed genes, 206 were highly correlated with stamen and pistil development. These genes include WM27B, DL, YAB1, YABBY4, WM 5, CER 1, and WBLH1, which have been implicated in flower development. The expression patterns of 25 differentially expressed genes were confirmed through quantitative real-time reverse transcription PCR.ConclusionsAnalysis of this transcriptome resource enabled us to characterize gene expression profiles, examine differential gene expression, and produce a candidate gene list related to wheat stamen and pistil development. This work is significant for the development of genomic resources for wheat, and provides important insights into the molecular mechanisms of wheat stamen and pistil development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1453-0) contains supplementary material, which is available to authorized users.

show abstract

“…Other chemicals that contribute to SAR have been identified (Gao et al, 2014;Kachroo & Robin, 2013;Shah & Zeier, 2013), including the methylated SA derivative MeSA, the dicarboxylic acid azelaic acid (AzA), and the phosphorylated sugar glycerol-3-phosphate (G3P). In addition, SAR is also dependent on the lipid-transfer-like proteins (LTPs;Champigny et al, 2011;Chanda et al, 2011;Yu et al, 2013).…”

Section: Systemic Acquired Resistancementioning

confidence: 99%

Induced resistance in wheat

Bellameche¹

View full text Add to dashboard Cite

During evolution, plants have developed a variety of chemical and physical defences to protect themselves from stressors. In addition to constitutive defences, plants possess inducible mechanisms that are activated in the presence of the pathogen. Also, plants are capable of enhancing their defensive level once they are properly stimulated with non-pathogenic organisms or chemical stimuli. This phenomenon is called induced resistance (IR) and it was widely reported in studies with dicotyledonous plants. However, mechanisms governing IR in monocots are still poorly investigated. Hence, the aim of this thesis was to study the efficacy of IR to control wheat diseases such leaf rust and Septoria tritici blotch. In this thesis histological and transcriptomic analysis were conducted in order to better understand mechanisms related to IR in monocots and more specifically in wheat plants. Successful use of beneficial rhizobacteria requires their presence and activity at the appropriate level without any harmful effect to host plant. In a first step, the interaction between Pseudomonas protegens CHA0 (CHA0) and wheat was assessed. Our results demonstrated that CHA0 did not affect wheat seed germination and was able to colonize and persist on wheat roots with a beneficial effect on plant growth. Once we showed the absence of side effects of CHA0 on wheat plants, in the second step, we evaluate efficacy of CHA0 or β-aminobutyric acid (BABA) to induce resistance against leaf rust caused by the biotroph Puccinia triticina in wheat. Our results confirmed the capacity of CHA0 to control leaf rust at the seedling stage. BABA showed dose-dependent reduction of leaf rust infection accompanied with plant growth repression at 20 mM. Balancing between protection and growth repression, a concentration of 15 mM was chosen as suitable dose for leaf rust control. Defence reactions such as callose deposition and H2O2 regeneration involved in the observed resistance were investigated. Both treatments reduced fungal penetration and haustoria formation of P. triticina with differences in timing and amplitude, leading to different levels of resistance to leaf rust. IR in wheat was accompanied with high deposition of callose and the accumulation of H2O2 during fungal infection, showing their importance in mechanisms involved in this resistance. To deeply clarify differences and similarities between CHA0- and BABA-IR at transcriptomic level, in the third step, the expression level of defence-related genes was analysed by RT-qPCR during IR induced by CHA0 and BABA against leaf rust. A correlation between induction of genes and P. triticina infection events was observed. A clear difference between the two induced responses is that BABA target more defence-related genes compared to CHA0 treatment. The last step was to evaluate the two mentioned elicitors (CHA0 and BABA), Pseudomonas chlororaphis PCL1391 and Benzothiadiazole (BTH) for their ability to induce resistance in wheat against the hemibiotrophic fungus Zymoseptoria tritici. Only BABA efficiently enhanced plant resistance to Z. tritici. In conclusion, exploiting IR might be a prominent strategy to control wheat disease. However, its effectiveness depends on the combination inducer/pathogen. Arguably, CHA0 bacteria and BABA induce similar defence reactions leading both to enhanced levels of resistance. However, only BABA enhanced defences against the hemibiotroph Z. tritici, suggesting that resistance of the plants react to the lifestyle of the pathogen and IR enhances does not involve necessarily all of them. More understanding is needed on both on capacity of the inducer to induce and the plant to become induced.

show abstract

Cloning and characterization of endo-β-1,4-glucanase genes in the common wheat line three pistils

Cited by 6 publications

References 33 publications

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

Pistillody mutant reveals key insights into stamen and pistil development in wheat (Triticum aestivum L.)

Induced resistance in wheat

Contact Info

Product

Resources

About