Expression levels of candidate genes for intramuscular fat deposition in two Banna mini-pig inbred lines divergently selected for fatness traits

Zhao, Sumei; Li, Weizhen; Pan, Hongbin; Huang, Ying; Yang, Moon-Sik; Wei, Hong‐Jiang; Shi-zheng, Gao

doi:10.1590/s1415-47572012005000079

Cited by 16 publications

(8 citation statements)

References 48 publications

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“…For the genome wide association study (GWAS), the PCs, the Norberg angle averaged over each dog, and the angle of inclination averaged over each dog were analyzed in a linear mixed model framework using the program GEMMA v.0.94 [33]. Only SNPs with minor allele frequency (MAF) > 0.05 were included in the analysis and a significance threshold of P < 3.5×10 −7 (the Bonferroni-adjusted genome wide P -value < 0.05) was used.…”

Section: Methodsmentioning

confidence: 99%

Genetic mapping of principal components of canine pelvic morphology

Fealey

Todhunter

et al. 2017

Canine Genet Epidemiol

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BackgroundConcentrated breeding effort to produce various body structures and behaviors of dogs to suit human demand has inadvertently produced unwanted traits and diseases that accompany the morphological and behavioral phenotypes. We explored the relationship between pelvic conformation and canine hip dysplasia (HD) because purebred dogs which are predisposed, or not, to HD share common morphologic features, respectively. Thirteen unique bilateral anatomical features of the pelvis were measured on 392 dogs of 51 breeds and 95 mixed breed dogs. Principal components (PCs) were derived to describe pelvic morphology. Dogs were genotyped at ~183,000 single nucleotide polymorphisms and their hip conformation was measured by the Norberg angle and angle of inclination between the femoral neck and diaphysis.ResultsNo associations reached genome wide significance for the Norberg angle when averaged over both hips. PC1 was negatively correlated with the Norberg angle (r = -0.31; P < 0.05) but not the angle of inclination (r = -0.08; P > 0.05). PC1, 2, 4, and 5 differed significantly between male and female dogs confirming pelvic sexual dimorphism. With sex as a covariate, the eigenvector contribution to PC1 reflected the overall size of the pelvis and was significantly associated with the IGF-1 locus, a known contributor to canine body size. PC3, which represented a tradeoff between ilial length and ischial length in which a longer ischium is associated with a shorter ilium, was significantly associated with a marker on canine chromosome 16:5181388 bp. The closest candidate gene is TPK1, a thiamine-dependent enzyme and part of the PKA complex. Associations with the remaining PCs did not reach genome wide significance.Conclusion IGF-1 was associated with the overall size of the pelvis and sex is related to pelvic size. Ilial/ischial proportion is genetically controlled and the closest candidate gene is thiamine-dependent and affects birth weight and development of the nervous system. Dogs with larger pelves tend to have smaller NAs consistent with increased tendency toward HD in large breed dogs. Based on the current study, pelvic shape alone was not strongly associated with canine hip dysplasia.Electronic supplementary materialThe online version of this article (doi:10.1186/s40575-017-0043-7) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Genetic mapping of principal components of canine pelvic morphology

Fealey

Todhunter

et al. 2017

Canine Genet Epidemiol

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“…For example, increased expression of FABP3 in the muscle of fat Banna mini‐pig inbred line than that in the lean line was reported by Zhao, Li, et al. () and Zhao, Tian, et al. ().…”

Section: Discussionmentioning

confidence: 75%

“…Higher expression levels of these two genes were related to higher IMF content or tender meat in different species. For example, increased expression of FABP3 in the muscle of fat Banna mini-pig inbred line than that in the lean line was reported by Zhao, Li, et al (2012) and Zhao, Tian, et al (2012). Similarly, significant difference in the expression of FABP3 were observed in the breast muscle of two lines of Beijing-You chickens selected for IMF content: UL (selected up) and DL (selected down; Li et al, 2013).…”

Section: Variability Of Expression Levels Of Genes In Relation To Imfmentioning

confidence: 88%

“…It encodes FA synthase, an important enzyme that catalyses the biosynthesis FAs. Differential expressions of FASN in muscle were reported within the same breed divergent for IMF content (in pig; Lim et al., ) and meat tenderness (in cattle; Zhao, Tian, et al., ; in pig; Zhao, Li, et al., ). Breed‐specific expression of FASN was also observed in pigs (Duran‐Montge, Theil, Lauridsen, & Esteve‐Garcia, ), cattle (Ji et al., ), and chicken (Cui et al., ).…”

Section: Discussionmentioning

confidence: 99%

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Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

Chen³

et al. 2018

Animal Physiology Nutrition

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In this study, meat quality traits were compared between squabs from two pigeon breeds: one Chinese indigenous breed, the Shiqi (SQ) meat-type pigeon, and an imported breed, the white king (WK) meat-type pigeon. Breed differences were detected in the content of intramuscular fat (IMF) in the breast muscle. SQ squabs had significantly higher IMF content than the WK birds. The shear force value (an objective measure of meat tenderness) of SQ birds was also relatively lower than that of the WK squabs. Further analysis of fatty acids profile revealed that SQ squabs exhibited significant advantage in the synthesis of polyunsaturated fatty acids, while WK squabs were significantly higher in the sum of monounsaturated fatty acids. Breast muscle in the SQ squabs was also significantly higher in the ratio of polyunsaturated fatty acids to saturated fatty acids, as well as the sum of omega 6 fatty acids. Variability of expression levels of functional genes in relation to fat accumulation and meat tenderness was analysed by qRT-PCR. Gene expression analyses showed that the hepatic expression of LPL (lipoprotein lipase), FABP4 (fatty acid-binding protein 4), and CAPN2 (calpain-2) were significantly higher in the SQ squabs. In the breast muscle tissue, the FABP3 (fatty acid-binding protein 3) and CAPN2mRNA abundance was significantly higher in SQ squabs. Our results suggested that these differentially expressed genes might be candidate genes used in the programmes of targeted selection for squabs with higher IMF content, tender meat, and more favourable fatty acids composition.

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“…Intramuscular fat (IMF) content is an important determinant of meat quality in the pig (Gerbens et al, 2000;Sanchez et al, 2007;Lee et al, 2010b), and is positively correlated with quality characteristics such as juiciness, flavor, and tenderness (Fernandez et al, 1999;Gerbens et al, 1999Gerbens et al, , 2000Zhao et al, 2012). Fatty acid (FA) composition serves as an indicator of lipid quality (Sanchez et al, 2007;Lee et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

Effect of UBE3C polymorphisms on intramuscular fat content and fatty acid composition in Duroc pigs

Supakankul

Mekchay

2016

Genet. Mol. Res.

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ABSTRACT. Ubiquitin protein ligase E3C (UBE3C) is involved in the ubiquitin-proteasome pathway, and several ubiquitin protein ligases are important for fat deposition and lipid metabolism. The objective of this study was to analyze the association between a single nucleotide polymorphism (SNP) of the UBE3C gene with intramuscular fat (IMF) content and fatty acid (FA) composition in Duroc pigs. Four SNP markers (g.1586399A>G, g.1591358G>A, g.1600132G>C, and g.1600166G>A) of porcine UBE3C were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method, and their associations with IMF content and FA composition were investigated in a commercial Duroc pig population. Two SNP markers (g.1586399A>G and g.1591358G>A) were segregated among the pigs. No UBE3C polymorphisms at g.1600132G>C or g.1600166G>A were observed. The UBE3C g.1586399A>G SNP was significantly associated with IMF content, while the UBE3C g.1591358G>A SNP was associated with palmitic, stearic, eicosenoic, and eicosadienoic acid levels, and saturated FA levels. These results suggest that polymorphisms in porcine UBE3C are correlated with IMF content and FA composition, and confirm the importance of porcine UBE3C as a candidate gene for fat deposition in pigs.

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Expression levels of candidate genes for intramuscular fat deposition in two Banna mini-pig inbred lines divergently selected for fatness traits

Cited by 16 publications

References 48 publications

Genetic mapping of principal components of canine pelvic morphology

Genetic mapping of principal components of canine pelvic morphology

Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

Effect of UBE3C polymorphisms on intramuscular fat content and fatty acid composition in Duroc pigs

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