Identification of RAPD and SCAR markers associated with yield traits in the Indian tropical tasar silkworm Antheraea mylitta drury

Abstract

The tropical tasar silkworm, Antheraea mylitta, is a semi-domesticated vanya silk-producing insect of high economic importance. To date, no molecular marker associated with cocoon and shell weights has been identified in this species. In this report, we identified a randomly amplified polymorphic DNA (RAPD) marker and examined its inheritance, and also developed a stable diagnostic sequence-characterized amplified region (SCAR) marker. Silkworms were divided into groups with high (HCSW) and low (LCSW) cocoon and shell weights, and the F2 progeny of a cross between these two groups were obtained. DNA from these silkworms was screened by PCR using 34 random primers and the resulting RAPD fragments were used for cluster analysis and discriminant function analysis (DFA). The clustering pattern in a UPGMA-based dendogram and DFA clearly distinguished the HCSW and LCSW groups. Multiple regression analysis identified five markers associated with cocoon and shell weights. The marker OPW16905 bp showed the most significant association with cocoon and shell weights, and its inheritance was confirmed in F2 progeny. Cloning and sequencing of this 905 bp fragment showed 88% identity between its 134 nucleotides and the Bmc-1/Yamato-like retroposon of A. mylitta. This marker was further converted into a diagnostic SCAR marker (SCOPW 16826 bp). The SCAR marker developed here may be useful in identifying the right parental stock of tasar silk-worms for high cocoon and shell weights in breeding programs designed to enhance the productivity of tasar silk.

Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst

“…In a previous study, Su et al (2007) developed a 'strain-specific' G. lucidum ISSR-based SCAR marker, which could identify certain strains of G. lucidum, but it was not a species-specific marker. RAPD-SCAR markers have been successfully developed for the identification of some other organisms, including plants, animals, and microbes (Jiang et al, 2009;Dutta et al, 2012;Lee et al, 2013;Yang et al, 2013, Fu et al, 2015Zhang et al, 2015;Mei et al, 2015).…”

Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning

“…According to the best of our knowledge, this is the first RAPD-SCAR study on L. japonica identification. However, some studies have reported the RAPD-SCAR technique for the identification and authentication of other organisms like pepper (Jiang, Jianhua, & Li, 2009), longan fruits (Yang et al, 2013), shrimp (Dutta et al, 2014), silkworm (Dutta et al, 2012), microbes (Lee, Lee, Lee & Lee, 2013) among others.…”

Population Diversity of Odontotermes formosanus (Shiraki) (Termitidae, Macrotermitinae) from Different Geographic Locations in Anhui Province, China

“…In addition, the features of long sequence and high annealing temperature ensure that ISSR-PCR would exhibit high stability and reproducibility (Nagaoka & Ogihara, 1997;Tsumura et al, 1996;Qian et al, 2001;Naik et al, 2017). Although ISSR is widely used in studies on various plants (Qian et al, 2001;Cao et al, 2006;Tanya et al, 2011), fungi (Menzies et al, 2003) and some insects Dutta et al, 2012;Barbosa et al, 2014), but it was less used on the study of termites (Long et al, 2009).…”