Site heteroplasmy in the mitochondrial cytochrome b gene of the sterlet sturgeon Acipenser ruthenus

Dudu, Andreea; Georgescu, Sergiu Emil; Berrebi, Patrick; Costache, Marieta

doi:10.1590/s1415-47572012005000058

Cited by 9 publications

(6 citation statements)

References 35 publications

(41 reference statements)

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“…Considering the maternal inheritance of the mitochondrial genome, these specimen identifications are as expected, because each sturgeon hybrid was found in the clades of their corresponding maternal genitor species. In sturgeons the phenomenon of site heteroplasmy in mtDNA has been previously encountered (Dudu et al, 2012) due to the possible presence of paternal mtDNA along with the maternal mtDNA known as paternal leakage (Guo et al, 2006). All the COI sequences obtained here did not show this phenomenon.…”

Section: Discussionmentioning

confidence: 45%

Use of DNA barcoding in the assignment of commercially valuable fish species from Romania

Popa

Dudu

Bănăduc

et al. 2017

Aquat. Living Resour.

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DNA barcoding is a molecular technique frequently used either for biodiversity monitoring and fish conservation or in the identification of the species of origin for raw and processed food from restaurants or fish markets. The most important aspect of this technique is that it works for all stages of life and can be used to distinguish between closely related taxa. Also, the technique has been used to unmask attempts of frauds in fish markets where more desirable and expensive fish are sometimes substituted with lower valued species. Our study aims to test the utility of the COI barcoding gene in the correct identification of several economically and ecologically valuable fish species, and interspecies hybrids, from Romanian wild fauna, aquaculture and fish markets. By combining the 122 COI sequences generated here with additional barcodes downloaded from GenBank and BOLD we screened several members of Acipenseriformes and Salmoniformes. While COI was able to correctly classify the majority of Salmoniformes, the identification process for Acipenseriformes proved more difficult, especially in the case of hybrids. Although the results show that DNA barcodes present a low degree of interspecies variation making classification possible only at the genus level, this technique is still relatively cheap, fast, and useful in the identification of incorrectly labelled market products.

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Section: Discussionmentioning

confidence: 45%

Use of DNA barcoding in the assignment of commercially valuable fish species from Romania

Popa

Dudu

Bănăduc

et al. 2017

Aquat. Living Resour.

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“…when mitochondrial genomes are easily distinguished, whereas cases of conspecific crosses might be easily overlooked due to greater similarity of subspecific mtDNA lineages (Mastrantonio et al 2019). In natural populations, heteroplasmic individuals have been documented for example in Drosophila flies (Nunes et al 2013;Wolff et al 2013; Dokianakis and Ladoukakis 2014) and in sterlet sturgeons, Acipenser ruthenus (Dudu et al 2012). Furthermore, paternal leakage has been documented from a tit hybrid zone (Kvist et al 2003) and from a water frog species of hybrid origin on the Balkan Peninsula (Radoji ci c et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial heteroplasmy in an avian hybrid form (Passer italiae: Aves, Passeriformes)

Päckert

Giacalone²,

Valvo

et al. 2019

Mitochondrial DNA Part B

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Mitochondrial heteroplasmy is the result from biparental transmission of mitochondrial DNA (mtDNA) to the offspring. In such rare cases, maternal and paternal mtDNA is present in the same individual. Though recent studies suggested that mtDNA heteroplasmy might be more common than previously anticipated, that phenomenon is still poorly documented and was mostly detected in case studies on hybrid populations. The Italian sparrow, Passer italiae is a homoploid hybrid form that occurs all across the Italian Peninsula mostly under strict absence of either of its parent species, the house sparrow (P. domesticus) and the Spanish sparrow (P. hispaniolensis). In this study, we document a new case of mitochondrial heteroplasmy from two island populations of P. italiae (Ustica and Lipari). Our analysis was based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) that allows for a clear distinction between mitochondrial lineages of the two parental species. We amplified and sequenced the mitochondrial ND2 gene with specifically designed primer combinations for each of the two parental species. In two of our study populations, a single individual carried two different ND2 haplotypes from each of the two parental lineages. These findings contribute to current knowledge on the still poorly documented phenomenon of paternal leakage in vertebrates.

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“…The PCR reactions were conducted with the GeneAmp 9700 PCR System (Applied Biosystems), in 25 μL final volume with 50 ng DNA template, 1X AmpliTaq Gold Reaction Buffer, 1.5 mM MgCl 2 , 200 μM of each dNTPs, 0.4 μM of each primer and 1 U of AmpliTaq Gold Polymerase (Applied Biosystems). For details about amplification see Dudu et al (2012). PCR products' sequencing was conducted with the ABI Prism 3130 Genetic Analyser (Applied Biosystems), using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and the BigDye n -number of individuals…”

Section: Mtdna Analysesmentioning

confidence: 99%

Genetic profile analysis of aquaculture Russian sturgeon (Acipenser gueldenstaedtii) – implications for restocking measures

Burcea¹,

Popa²,

Florescu³

et al. 2017

Acta Zool Acad Sci H

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The aim of this study is to help the conservation effort of the Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833 population from Lower Danube, ensuring its sustainable development in regards to aquaculture and wild populations. We evaluated the genetic diversity of five aquaculture strains using molecular markers, determining the suitability of employing the individuals in ex situ conservation and in restocking programs. The mitochondrial cytochrome b gene analysis highlighted that some sequences from aquaculture and wild populations are grouped together representing a single haplotype. Results showed that the five aquaculture strains are grouped in 3 haplotypes with a haplotype diversity of 0.55686. The microsatellite analysis highlights that the majority of loci have a tetrasomic profile that makes the inferring of genotypes and the statistical analyses difficult, only two (AciG198 and Spl106) had a disomic profile that could prove useful in future studies. We can conclude that the molecular analysis is important for the genetic characterization of aquaculture strains, shedding light on the genetics of A. gueldenstaedtii.

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Site heteroplasmy in the mitochondrial cytochrome b gene of the sterlet sturgeon Acipenser ruthenus

Cited by 9 publications

References 35 publications

Use of DNA barcoding in the assignment of commercially valuable fish species from Romania

Use of DNA barcoding in the assignment of commercially valuable fish species from Romania

Mitochondrial heteroplasmy in an avian hybrid form (Passer italiae: Aves, Passeriformes)

Genetic profile analysis of aquaculture Russian sturgeon (Acipenser gueldenstaedtii) – implications for restocking measures

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