Phylogenetic position of an uncharacterized Brazilian strain of bovine papillomavirus in the genus Xipapillomavirus based on sequencing of the L1 open reading frame

Abstract

The use of PCR assays with degenerate primers has suggested the existence of numerous as yet uncharacterized bovine papillomaviruses (BPV). Despite the endemic nature of BPV infections, the identification of BPV types in Brazilian cattle is still only sporadic. However, in a recent analysis of a partial segment of the L1 gene, we observed notable diversity among the BPV types detected. The aim of this study was to determine the phylogenetic position of the previously identified wild strain BPV/BR-UEL2 detected in the state of Paraná in Brazil. Since previous analysis of the partial L1 sequence had shown that this strain was most closely related to BPV type 4, genus-specific primers were designed. Phylogenetic analysis using complete L1 ORF sequences revealed that BPV/BR-UEL2 was related to BPV types classified in the genus Xipapillomavirus and shared the highest L1 nucleotide sequence similarity with BPV type 4 (78%). This finding suggests that BPV/BR-UEL2 should be classified as a potential new type of BPV in the genus Xipapillomavirus.

Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle

“…Reports available from different regions of the country indicate a significant diversity of viral types in Brazil, implying significant economic losses due to the associated diseases Lindsey et al, 2009;Lunardi et al, 2010). Of note, the putative BPV type BR-UEL-4 under accession No.…”

Bovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method

“…BPVs have been previously detected in Brazil [21], but the extent of the impact of BPVs associated diseases, both in dairy and cattle herds, needs further studies. Available reports in different regions of the country indicate a significant diversity of viral types among the Brazilian herd, implying an evident disease burden [18, 22–25]. Thus, the improvement of knowledge concerning the diagnosis and related clinical aspects of different BPV types among the Brazilian herd should be considered in the development of new sanitary measures, aiming to the prevention of BPV infection and its consequences.…”

Teat papillomatosis associated with bovine papillomavirus types 6, 7, 9, and 10 in dairy cattle from Brazil

“…Despite the high number of cattle herds affected by cutaneous papillomatosis in several geographical regions of Brazil, studies investigating BPV diversity associated with different clinical outcomes are sporadic ( Stocco dos Santos et al , 1998 ; Freitas et al , 2003 ; Wosiacki et al , 2005 ; Wosiacki et al , 2006 ; Claus et al , 2008 ; Claus et al , 2009b ; Claus et al , 2009c ; Lunardi et al , 2010 ).…”