Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA

Labate, Mônica Teresa Veneziano; Bertolo, Ana L.; Nascimento, Daniela Defavari do; Gutmanis, Gunta; Andrade, Alexander de; Calderan-Rodrigues, Maria Juliana; Camargo, Eduardo Leal Oliveira; Boaretto, Luis Felipe; Moon, David Henry; Bragatto, Juliano; Labate, Carlos Alberto

doi:10.1590/s1415-47572010005000078

Cited by 9 publications

(9 citation statements)

References 32 publications

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“…Analysis on endogenous expression of a UGDH in Eucalyptus grandis (GenBank accession number: EF179384) showed that it was mainly expressed in roots, stem and bark of 6-month-old saplings, with lower expression level in leaves [55]. In C .…”

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confidence: 99%

Transcriptome Sequencing of Codonopsis pilosula and Identification of Candidate Genes Involved in Polysaccharide Biosynthesis

et al. 2015

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Background Codonopsis pilosula (Franch.) Nannf. is one of the most widely used medicinal plants. Although chemical and pharmacological studies have shown that codonopsis polysaccharides (CPPs) are bioactive compounds and that their composition is variable, their biosynthetic pathways remain largely unknown. Next-generation sequencing is an efficient and high-throughput technique that allows the identification of candidate genes involved in secondary metabolism.Principal FindingsTo identify the components involved in CPP biosynthesis, a transcriptome library, prepared using root and other tissues, was assembled with the help of Illumina sequencing. A total of 9.2 Gb of clean nucleotides was obtained comprising 91,175,044 clean reads, 102,125 contigs, and 45,511 unigenes. After aligning the sequences to the public protein databases, 76.1% of the unigenes were annotated. Among these annotated unigenes, 26,189 were assigned to Gene Ontology categories, 11,415 to Clusters of Orthologous Groups, and 18,848 to Kyoto Encyclopedia of Genes and Genomes pathways. Analysis of abundance of transcripts in the library showed that genes, including those encoding metallothionein, aquaporin, and cysteine protease that are related to stress responses, were in the top list. Among genes involved in the biosynthesis of CPP, those responsible for the synthesis of UDP-L-arabinose and UDP-xylose were highly expressed.SignificanceTo our knowledge, this is the first study to provide a public transcriptome dataset prepared from C. pilosula and an outline of the biosynthetic pathway of polysaccharides in a medicinal plant. Identified candidate genes involved in CPP biosynthesis provide understanding of the biosynthesis and regulation of CPP at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing of Codonopsis pilosula and Identification of Candidate Genes Involved in Polysaccharide Biosynthesis

et al. 2015

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“…In woody tissues, the role of UGPase and UGP is poorly understood. Two UGDH genes mainly expressed in roots, stem and bark of 6-month-old E. grandis were cloned [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Integrated analysis of gene expression from carbon metabolism, proteome and metabolome, reveals altered primary metabolism in Eucalyptus grandis bark, in response to seasonal variation

et al. 2016

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BackgroundSeasonal variation is presumed to play an important role in the regulation of tree growth, especially for Eucalyptus grandis, a fast-growing tree. This variation may induce changes in the whole tree at transcriptional, protein and metabolite levels. Bark represents an important group of tissues that protect trees from desiccation and pathogen attack, and it has been identified as potential feedstock for lignocellulosic derived biofuels. Despite the growing interest, little is known about the molecular mechanisms that regulates bark metabolism, particularly in tropical countries.ResultsIn this study we report the changes observed in the primary metabolism of E. grandis bark during two contrasting seasons in Brazil, summer (wet) and winter (dry), through the combination of transcripts (RT-qPCR), proteome (2-DE gels) and metabolome (GC-MS) analysis, in an integrated manner. Twenty-four genes, involved in carbon metabolism, were analyzed in the two seasons. Eleven were up-regulated in summer, three were up-regulated in winter and ten did not show statistical differences in the expression pattern. The proteomic analysis using 2-DE gels showed 77 proteins expressing differences in abundance, with 38 spots up-regulated in summer and 37 in winter. Different metabolites significantly accumulated during winter.ConclusionsThis study revealed a metabolic reconfiguration in the primary metabolism of E. grandis bark, triggered by seasonal variation. Transcripts and protein data suggests that during winter carbohydrate formation seems to be favored by tree metabolism. Glucose, fructose and sucrose accumulated at significant levels during the winter.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0839-8) contains supplementary material, which is available to authorized users.

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“…Adenylyl cyclase associated protein, envolvida no processo de elongação das fibras (KAWAI et al, 1998); Alpha-1,4-glucan-protein synthase [UDP-forming], uma proteína se-wap41 associada ao complexo de Golgi, que está relacionada à formação dos plasmodesmas (SAGI et al, 2005), UDP-glucose dehydrogenase (UGDH), envolvida na biossíntese de hemiceluloses (LABATE et al, 2008) e a Caffeoyl-CoA Omethyltransferase (CCoAOMT), envolvida na biossíntese de lignina (COSTA et al, 1999…”

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“…A utilização da técnica de eletroforese bidimensional associada à espectrometria (LABATE et al, 2008).…”

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“…No presente trabalho, quatro spots apresentaram similaridade com a UGDH indicando uma possível existência de 4 isoformas da proteína(Figura 17). Dessa forma, o resultado encontrado sugere que a enzima UGDH exerce um papel fundamental na formação e qualidade da madeira de eucalipto(LABATE et al, 2008). Imagem ampliada do gel bidimensional da região cambial de árvores de eucalipto com 22 anos.…”

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Análise proteômica da região cambial de árvores adultas de Eucalyptus grandis

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Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA

Cited by 9 publications

References 32 publications

Transcriptome Sequencing of Codonopsis pilosula and Identification of Candidate Genes Involved in Polysaccharide Biosynthesis

Transcriptome Sequencing of Codonopsis pilosula and Identification of Candidate Genes Involved in Polysaccharide Biosynthesis

Integrated analysis of gene expression from carbon metabolism, proteome and metabolome, reveals altered primary metabolism in Eucalyptus grandis bark, in response to seasonal variation

Análise proteômica da região cambial de árvores adultas de Eucalyptus grandis

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