Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene

Abstract

Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.

Membrane Associated Progesterone Receptors: Promiscuous Proteins with Pleiotropic Functions – Focus on Interactions with Cytochromes P450

“…Despite its structural similarities to PGRMC1, PGRMC2 has been less well studied (Wendler and Wehling, 2013; Hasegawa et al, 2016). Expression appears to be ubiquitous with similar intracellular localization (Chen et al, 2010; Intlekofer and Petersen, 2011). In SKOV-3 ovarian cancer cells no differences between PGRMC1 and 2 in regard to cell viability or response to cisplatin and progesterone were found, but only PGRMC2 inhibited cell migration (Albrecht et al, 2012).…”

Neudesin as a unique secreted protein with multi-functional roles in neural functions, energy metabolism, and tumorigenesis

“…However, PGRMC1 binds heme, not progesterone (Cahill, 2007 ). PGRMC1 and PGRMC2 are mainly located in the endoplasmic reticulum (Gerdes et al, 1998 ; Chen et al, 2010 ). The expression of PGRMC1 is up-regulated in cancer cells.…”

A novel progesterone receptor membrane component (PGRMC) in the human and swine parasite Taenia solium: implications to the host-parasite relationship

“…These analyses were performed with bio-computer hardware like EAnnot, SNAP and FgenesH, which in turn are based on algorithms able to find preserved regions including exon-intron junction sites, and polyadenylated sequences. Notably, transcription of both exon-intron junction sites and polyadenylated sequences are partially regulated by the predicted form of the PGRMC [ 41 ]. As mentioned in the result section, this sequence 5′ and 3′ seems to have lost the non-coding regions (UTR’s) at the end as well polyadenylation sites [ 41 ].…”

“…Notably, transcription of both exon-intron junction sites and polyadenylated sequences are partially regulated by the predicted form of the PGRMC [ 41 ]. As mentioned in the result section, this sequence 5′ and 3′ seems to have lost the non-coding regions (UTR’s) at the end as well polyadenylation sites [ 41 ].…”