Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats

Deng, Changjian; Ma, Rongnuan; Yue, Xiumei; Lan, Xianyong; Chen, Hong; Lei, Chuzhao

doi:10.1590/s1415-47572010005000034

Cited by 26 publications

(13 citation statements)

References 19 publications

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“…However, contrary to the present findings, [ 9 ] in Boer goat bucks reported significant effect of genotype on chest girth at birth and weaning; [ 15 ] in Nanjiang Huang goat found significant effect of genotype on chest girth at 2 months of age and [ 16 ] in Guanzhong goat with IGF-I gene (a gene similar to GH gene in its effect) polymorphism reported significant (p<0.05) effect of genotype on chest circumference. The least squares analysis of variance ( Table-1 ) conducted to analyze association of polymorphic variants of GH gene with paunch girth at birth to 180 days of age evinced highly significant effect (p<0.01) of breed on paunch girth at each stage.…”

Section: Resultscontrasting

confidence: 99%

Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat

Singh¹,

Tomar²,

Thakur³

et al. 2015

Vet World

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Aim:The aim was to study the polymorphism of exon 2 and exon 3 of growth hormone (GH) gene, to test the polymorphic variants for Hardy–Weinberg equilibrium and to investigate association of these polymorphisms with chest girth and paunch girth in Sirohi and Barbari breeds of goat.Materials and Methods:A total of 80 kids involving forty each of Sirohi and Barbari breeds of goat were included in the study. A good quality genomic DNA isolated from the whole blood using standard protocol were used for polymerase chain reaction (PCR) amplification and products obtained on restriction digestion of amplicon with enzyme HaeIII were separated on 2% agarose gel, and documented in a gel doc system. The chest girth and paunch girth of kids at birth and weekly intervals up to 4 weeks of age and subsequently at 2 months, 3 months and 6 months of age were recorded. Allele frequency and genotype distribution of polymorphism were tested for Hardy–Weinberg equilibrium by program me Genepop package. Association between different genetic variants on chest girth and paunch girth were analyzed by least squares analysis employing suitable statistical model.Results:The PCR product of genomic DNA isolated from kids of Sirohi and Barbari breeds of goat on digestion with the restriction enzyme HaeIII revealed two genotypic variants viz., AB and BB. None of the two breeds was in Hardy–Weinberg equilibrium for these variants. The least squares analysis of variance revealed non-significant effect of GH genotype and breed × genotype interaction on chest girth and paunch girth from birth to 180 days of age. The effect of breed was highly significant (p<0.01) at all ages.Conclusion:The present study showed that both the breeds were polymorphic at the exon 2 and exon 3 loci of GH gene under study with respect to HaeIII restriction endonuclease. None of the breeds was in Hardy–Weinberg equilibrium for this region of GH gene. In the present study, no significant association between GH genotype and chest girth and paunch girth could be established but comparatively higher chest girth and paunch girth were observed for AB genotype across the breeds.

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Section: Resultscontrasting

confidence: 99%

Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat

Singh¹,

Tomar²,

Thakur³

et al. 2015

Vet World

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show abstract

“…In our study, the frequency of mutant allele (B) was low (0.35) as compared to 0.65 frequency of wild allele (A), therefore, the BB genotype was observed in a small proportion of samples. Likewise, same mutation was observed in Guanzhong and Xinong-Saanen breeds by (Deng et al, 2010). Additionally, the frequency of mutant allele was low in both of goat breeds but in contrast to our findings, this polymorphism was not associated with any of growth traits.…”

Section: Growth Hormone Gene (Gh)supporting

confidence: 51%

Polymorphisms in Growth Hormone (Gh) and Insulin-Like Growth Factor-1 (Igf-1) Gene and Their Association With Growth Traits in Beetal Goat

Shareef¹

2018

PAKJAS

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Beetal is one of the most famous goat breed of Pakistan which is also present in parts of Indian Punjab. It is popular for its larger body size, higher body weight, and good ability to produce milk. In current study, polymorphism in Growth Hormone (GH) and Insulin-like Growth Factor-1 (IGF-1) genes and their association with growth-related traits were studied in Beetal goat. On the basis of previously reported mutations, a set of 7 primer pairs were designed to screen PCR-RFLP based polymorphisms in 60 random selected Beetal goats varying in body weight. The IGF-1 gene was found polymorphic and following three genotypes (AA, AB, and BB) were detected in tested animals with the frequency of mutant (B) and wild-type (A) alleles as 0.35 and 0.65 respectively. Homozygous carrier genotype (BB) was significantly associated (P<0.05) with higher body weight (47.13±0.78kg) followed by heterozygous carrier (BB) and wild-type (AA) genotype associated with 40.45±0.31kg and 38.18±0.37kg body weight respectively. Additionally, maximum wither height and body length were observed in BB (32.25 ± 0.33, 31.93±0.27 inches) genotype followed by AB (31.93±0.27, 31.16±0.35) and AA (31.62±0.68, 30.87±0.86) genotype. Moreover, maximum heart girth was also observed in BB (33.65±0.11) genotype. All known point mutations of GH gene were found monomorphic in the tested animals. Upon sequencing of GH gene, one non-synonymous SNP was identified in exon 2 at 825bp position that caused a change of amino acid Threonine into Alanine. And likewise one insertion variant in intron 4 was observed at position 1546bp in this gene. The results showed that homozygous carrier animals, having BB genotype in IGF-1 gene, were having the highest values for growth traits suggesting the additive effects of mutant (B) allele on growth traits in this breed. On the basis of our results it is suggested that the identified markers can be used in marker-assisted-selection (MAS) in Beetal goat.

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“…These genes involved in the biological process GO terms are not master gene such as IGF1 , BMP2 or GH1 that directly affect body size as previously reported in mouse [ 27 ], dog [ 28 ], goat [ 29 ], pig [ 30 ] and human [ 31 ]. However, the genes identified in the study as having signatures of selection detected by XP-EHH method are related to the growth and body size.…”

Section: Discussionmentioning

confidence: 81%

Exploring the Genetic Signature of Body Size in Yucatan Miniature Pig

et al. 2015

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Since being domesticated about 10,000–12,000 years ago, domestic pigs (Sus scrofa domesticus) have been selected for traits of economic importance, in particular large body size. However, Yucatan miniature pigs have been selected for small body size to withstand high temperature environment and for laboratory use. This renders the Yucatan miniature pig a valuable model for understanding the evolution of body size. We investigate the genetic signature for selection of body size in the Yucatan miniature pig. Phylogenetic distance of Yucatan miniature pig was compared to other large swine breeds (Yorkshire, Landrace, Duroc and wild boar). By estimating the XP-EHH statistic using re-sequencing data derived from 70 pigs, we were able to unravel the signatures of selection of body size. We found that both selections at the level of organism, and at the cellular level have occurred. Selection at the higher levels include feed intake, regulation of body weight and increase in mass while selection at the molecular level includes cell cycle and cell proliferation. Positively selected genes probed by XP-EHH may provide insight into the docile character and innate immunity as well as body size of Yucatan miniature pig.

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Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats

Cited by 26 publications

References 19 publications

Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat

Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat

Polymorphisms in Growth Hormone (Gh) and Insulin-Like Growth Factor-1 (Igf-1) Gene and Their Association With Growth Traits in Beetal Goat

Exploring the Genetic Signature of Body Size in Yucatan Miniature Pig

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